Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,268,367	C→T	S68F (TCT→TTT)	kdsA →	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,268,367	0	C	T	100.0%	48.4 / NA	15	S68F (TCT→TTT)	kdsA	3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/4);  total (11/4)

CAAGATTAAGGCGATCCTATGAAACAAAAAGTGGTTAGCATTGGCGACATCAACGTAGCAAATGACCTGCCGTTCGTACTGTTTGGCGGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTCTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGCCATGGCGAAAACCGGTGCGGTAATTAACGTCAAGAAACCACAGTTTGTCAGCCCGGGACAGATGG  >  NC_000913/1268147‑1268609                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   cAAGATTAAGGCGATCCTATGAAACAAAAAGTGGTTAGCATTGGCGACATCAACGTAGCAAATGACCTGCCGTTCGTACTGTTTGGCGGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGAt                                                                                                                                                                                                                      <  1:91079/251‑1 (MQ=255)       tAAGGCGATCCTATGAAACAAAAAGTGGTTCGCCTTGGCGACATCAACGTAGCAAATGACCTGCCGTTCGTACTGTTTGGCGGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAACGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAAt                                                                                                                                                                                                                >  1:288089/1‑251 (MQ=255)              atcctccgaacccgaaaacggtaaGCATTGGCACCATGAACGTAGCAAATGACCCGACGTTCCTACTGTTTGCCAGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTCCGTAACTATGCCCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCATCTTTTGACAAAGCCAACCCCTCCTCCATCCCCTTTTATCGTGGACCGGGCCTGGAAGACGGGATGAAAATCTTcc                                                                                                                                                                                                           >  2:290508/24‑249 (MQ=255)                                     aGCATTGGCGACATCAACGTAGCAAATGACCTGCCGTTCGTACTGTTTGGCGGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGt                                                                                                                                                                                  >  2:21539/1‑251 (MQ=255)                                      gCATTGGCGACATCAACGTAGCAAATGACCTGCCGTTCGTACTGTTTGGCGGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTg                                                                                                                                                                                 <  2:116236/251‑1 (MQ=255)                                                               tGACCTGCCGTTCGTACTGTTTGGCGGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAAc                                                                                                                                                        >  1:4527/1‑251 (MQ=255)                                                                                                      ggAATCTCGCGCTCTGGCGATGCACATTTGCGAGCACTACGTAACTGTGACACAGACACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGCGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCACGTCAGGCACAGCCCGTTGCTGATGTCGTGGAtgtg                                                                                                                  >  2:147438/1‑250 (MQ=255)                                                                                                                gATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAACATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGcc                                                                                                       >  1:49548/1‑251 (MQ=255)                                                                                                                                  tGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGcc                                                                                      >  1:118468/1‑250 (MQ=255)                                                                                                                                                     tgACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGcc                                                                  >  1:72171/1‑251 (MQ=255)                                                                                                                                                                 tGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGTGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGCCATGGCGAAAAcc                                                      >  2:209541/1‑251 (MQ=255)                                                                                                                                                                               gtgtTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACCTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGCCATGGCGAAAACCGGTGCGGTAATTa                                         >  1:113939/1‑250 (MQ=255)                                                                                                                                                                                                     aaGCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGCCATGGCGAAAACCGGTGCGGTAATTAACGTCAAGAAACCACAGTTTGTc                  <  1:297433/251‑1 (MQ=255)                                                                                                                                                                                                       gCCAACCGCTCCTCCATCCACTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGCCATGGCGAAAACCGGTGCGGTAATTAACGTCAAGAAACCACAGTTTGTc                  >  1:110446/1‑249 (MQ=255)                                                                                                                                                                                                                     catccaCTTTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGCCATGGCGAAAACCGGTGCGGTAATTAACGTCAAGAAACCACAGTTTGTCAGCCCGGGACAGATgg  <  2:124189/251‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   CAAGATTAAGGCGATCCTATGAAACAAAAAGTGGTTAGCATTGGCGACATCAACGTAGCAAATGACCTGCCGTTCGTACTGTTTGGCGGTATGAACGTGTTGGAATCTCGCGATCTGGCGATGCGCATTTGCGAGCACTACGTAACTGTGACCCAGAAACTGGGTATCCCTTACGTGTTCAAAGCCTCTTTTGACAAAGCCAACCGCTCCTCCATCCACTCTTATCGTGGACCGGGCCTGGAAGAAGGGATGAAAATCTTCCAGGAGTTGAAGCAGACTTTTGGCGTGAAAATTATCACCGACGTTCACGAACCAAGTCAGGCACAGCCCGTTGCTGATGTCGTGGATGTGATTCAGTTGCCGGCGTTTCTTGCTCGCCAGACTGACCTGGTTGAAGCCATGGCGAAAACCGGTGCGGTAATTAACGTCAAGAAACCACAGTTTGTCAGCCCGGGACAGATGG  >  NC_000913/1268147‑1268609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑