Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,250,264	C→T	G192D (GGT→GAT)	dhaK ←	dihydroxyacetone kinase subunit K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,250,264	0	C	T	100.0%	73.6 / NA	23	G192D (GGT→GAT)	dhaK	dihydroxyacetone kinase subunit K
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/10);  total (13/10)

GGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTT  >  NC_000913/1250027‑1250505                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  ggTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGTTACAGGCATCGAGAGCGATAcc                                                                                                                                                                                                                                      <  2:137548/251‑1 (MQ=255)          gtttgTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTg                                                                                                                                                                                                                             >  1:55198/1‑251 (MQ=255)                 gttCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTg                                                                                                                                                                                                                       >  2:37172/1‑250 (MQ=255)                        cTGCCAACCGCCTTGTCGATAATCCCAGAAACGAACAGTGCGATGGCCTGAGCAATTTACCAGCAGGGTGTCGCACTTTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGACTGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAACGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCCTCGAGAGCGATACCTATTGCGTGGCCTTGattatta                                                                                                                                                                                                                >  2:305102/1‑249 (MQ=255)                                                                         gAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTccgc                                                                                                                                                              >  2:294451/1‑251 (MQ=255)                                                                                      gcagGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACg                                                                                                                                                 <  1:176919/251‑1 (MQ=255)                                                                                                  aCATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAAtt                                                                                                                                     <  1:162174/251‑1 (MQ=255)                                                                                                                           ggAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGGCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGCTGGCAACGCCGCGTc                                                                                                            <  2:299192/251‑1 (MQ=255)                                                                                                                               gagaAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCCCAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGccc                                                                                                        >  2:277415/1‑251 (MQ=255)                                                                                                                               gagaAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGccc                                                                                                        >  1:165431/1‑251 (MQ=255)                                                                                                                                    ggggCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGGAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACTCCGCGTCGCCCGGCAg                                                                                                   <  1:230419/251‑1 (MQ=255)                                                                                                                                     gggCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGt                                                                                                  <  1:51980/251‑1 (MQ=255)                                                                                                                                         ggcggTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGtata                                                                                               >  1:156679/1‑250 (MQ=255)                                                                                                                                            ggTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATaa                                                                                              >  1:286779/1‑248 (MQ=255)                                                                                                                                                     ccGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCtttt                                                                                  >  2:54402/1‑251 (MQ=255)                                                                                                                                                              cATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTCCCCGCGGCAGGAACGGTACAGGCATCGGGAGCGATGCCTATTGAGGGGCCTTGATGATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCGCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGTGTCGCCCGGCTGTATAAAGACTGTCTCTTACCGCAAcg                                                                         <  2:278926/251‑1 (MQ=255)                                                                                                                                                                 gAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAAcgtcg                                                                      >  2:38753/1‑251 (MQ=255)                                                                                                                                                                           gCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATg                                                             >  1:12031/1‑250 (MQ=255)                                                                                                                                                                                                  ccAGGGTAAAAGAAGGTTTGCCAGCGGCAGGAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTAcgccg                                     >  1:295693/1‑251 (MQ=255)                                                                                                                                                                                                                                gAACGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCa       <  2:18960/251‑1 (MQ=255)                                                                                                                                                                                                                                gAACGGAACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCCCTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTc        >  2:224627/1‑250 (MQ=255)                                                                                                                                                                                                                                  aCGGTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCaaa     <  1:54402/251‑1 (MQ=255)                                                                                                                                                                                                                                     gTACAGGCATCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGtt  <  1:37172/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  GGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAGAGAAGGGGCGGCGGTCAATACCCGGCTCACCATGAATGCCGACGCCAAACTCCATCTCATTATCCGCCAGGGTAAAAGAAGGTTTGCCCGCGGCAGGAACGGTACAGGCACCGAGAGCGATACCTATTGAGTGGCCTTGATTATTCAGCTTACGCCCCAGTTCCGCACAGGCGTCCAGTGAGTCGCCACGCTCCGCCGCTGCGCCTACGAGTTTTTCAATTAATACGGTGTTGGCAACGCCGCGTCGCCCGGCAGTATAAAGACTGTCTTTTACCGCAACGTCGTCATCAATGACCACAGTGGTCACTTTTACGCCGCTATCGTGCAGTAACTCGGTCGCTGTTTCAAAGTT  >  NC_000913/1250027‑1250505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑