Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,235,755 C→T S496N (AGC→AAC)  ycgB ← PF04293 family protein YcgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,235,7550CT94.4% 51.5 / NA 18S496N (AGC→AAC) ycgBPF04293 family protein YcgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/12);  minor base G (1/0);  total (6/12)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

ACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCATTATGAA  >  NC_000913/1235515‑1235995
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
aCCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCCCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCtt                                                                                                                                                                                                                                         <  1:180065/250‑1 (MQ=255)
                       aGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGcatcacat                                                                                                                                                                                                                 <  1:13490/251‑1 (MQ=255)
                             ggCGCGCACGAGCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATc                                                                                                                                                                                                           <  2:4486/251‑1 (MQ=255)
                                    aCGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCTCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTGCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAg                                                                                                                                                                                                    <  2:255769/251‑1 (MQ=255)
                                              gtACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACCGGCGATGcac                                                                                                                                                                                           >  1:219427/1‑250 (MQ=255)
                                                                    gggCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTgcgc                                                                                                                                                                     <  1:113301/250‑1 (MQ=255)
                                                                      gCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAAAGTTTCAGCAACTCGATTGTGTCGTCTTCGTTTTGCTGTTCGAGCATCACCACAAATCCCCACAGGCGAAGCAAACGCTTCAGGACTTCTTTgcgcacc                                                                                                                                                                  >  2:262111/1‑248 (MQ=255)
                                                                        aTGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCg                                                                                                                                                                <  1:85455/251‑1 (MQ=255)
                                                                                    agaTTTGGCACCGGATGCAGAAACATCTGCCGGGTGATTTAGCCATTCAGGGGCGCTAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTgc                                                                                                                                                    >  1:267301/1‑251 (MQ=255)
                                                                                                                gCCGGGTGATTTAGCCATTCAGGGGTGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTCCAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCTCAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTc                                                                                                                        <  1:167519/251‑1 (MQ=255)
                                                                                                                                                       gTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCTAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGa                                                                                 <  1:88074/251‑1 (MQ=255)
                                                                                                                                                                             aCGAGGGGTTTTTTGTTTTTGTTACGGATTTCCCATTCTGTGCGTGCAACGGTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATTCTTCAGGATTTCTTTGCGTCCCCGATGCAGCGGTTCGCGATTATGTGTAATATAACGCATCGTCAGCTAACGGTCGCCGCGCAAATCCACGTTCCCGATGTGAATATTCTGCTCCATATTACTTa                                                           <  2:133419/251‑1 (MQ=255)
                                                                                                                                                                              cGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTa                                                           >  1:9602/1‑250 (MQ=255)
                                                                                                                                                                               gAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGAAACTCGATGTTGCCGTCTTCATTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTa                                                           >  2:152791/1‑249 (MQ=255)
                                                                                                                                                                                   ggTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTtat                                                     <  1:71416/251‑1 (MQ=255)
                                                                                                                                                                                                            cccATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGAt                            >  1:199194/1‑251 (MQ=255)
                                                                                                                                                                                                             ccATTCTTGGCGGGCAACGTTCCAGCAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGAtc                           <  2:75917/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cAACTCGATGTTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCATTATGaa  <  2:8566/251‑1 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
ACCACAAACTGTCGGCGTTGTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCCCCCGATCCAGCGGTGCGCGATTATGTGGAATATAACGCAGCGTCAGCGAACGGTCGCCGCGCAAATCCACGTTCCAGATCTGAATATTCGGCTCCAGATTACTTAAGTTATATTGCGACGATAACCGGTTACGGATCTCCCGATAACCTTCTTCATTATGAA  >  NC_000913/1235515‑1235995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑