Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,683	C→T	N289N (AAC→AAT)	carB →	carbamoyl‑phosphate synthetase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,683	0	C	T	100.0%	83.7 / NA	25	N289N (AAC→AAT)	carB	carbamoyl‑phosphate synthetase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

ATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGA‑ACGACATCACTGGCGGACG‑TACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTC  >  NC_000913/31436‑31902                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               atgaGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCg                                                                                                                                                                                                                             <  2:81478/251‑1 (MQ=255)                 cTGGATAGAGTCCGAGCTGGCAGTGCTGAGTGATAAAAACGCCAACTGCATCATCGTCTGCTCTATCGACAACTTAGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACAGACAAAGAATATCAAATCATGAGTAACGCCTCGATGGCGGTGCTGCGTGCAATCGGCGTTGAAACCGGTGGTTCCAAAGTTACGTTTGCGGTGAATCCGAAAAACGGTCGTCTg                                                                                                                                                                                                              >  2:21079/1‑250 (MQ=255)                   ggAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCAGCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGAt                                                                                                                                                                                                            <  2:112195/250‑1 (MQ=255)                                   ggAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAcc                                                                                                                                                                                           <  1:252830/251‑1 (MQ=255)                                   ggAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAcc                                                                                                                                                                                           >  2:40877/1‑251 (MQ=255)                                         ggtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAcgcg                                                                                                                                                                                     <  2:81133/251‑1 (MQ=255)                                              gTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGTGCCTGCCCAAACGCTGACCGACAAAGAATATCATATCATGCGTAACGCCTCGATGGCTGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGGGTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTcc                                                                                                                                                                                <  2:258340/251‑1 (MQ=255)                                                gATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCg                                                                                                                                                                              >  2:299982/1‑251 (MQ=255)                                                    aaaaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttct                                                                                                                                                                          <  2:218809/251‑1 (MQ=255)                                                     aaaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGCAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttctt                                                                                                                                                                         >  1:43634/1‑251 (MQ=255)                                                      aaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                                                        <  2:43044/251‑1 (MQ=255)                                                             aCTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTg                                                                                                                                                                 <  1:17220/251‑1 (MQ=255)                                                             aCTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTg                                                                                                                                                                 >  2:46072/1‑251 (MQ=255)                                                                       cGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAGCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                                                                        <  1:283178/250‑1 (MQ=255)                                                                        gTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGc                                                                                                                                                      >  1:294053/1‑251 (MQ=255)                                                                         tCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCACGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                                                                     >  1:270097/1‑251 (MQ=255)                                                                            gCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                                                                    <  1:106649/249‑1 (MQ=255)                                                                                       aaCTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGAt                                                                                                                                       >  1:54578/1‑251 (MQ=255)                                                                                                                          cATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTTCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAATGGCgg‑gggggtga                                                                                                    >  2:160502/1‑246 (MQ=255)                                                                                                                                   cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTcgac                                                                                           <  1:46072/250‑1 (MQ=255)                                                                                                                                                aagCTGACCGACACAGAATATCAAATCATGCGTAACGCCTCGATGGCGGCGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGGTTCCCGATTGCTAAAGTGGCGGCGCAACTGGCGGGTGGGT‑ACAcccctcacggaacggagca                                                                                >  2:202471/3‑232 (MQ=255)                                                                                                                                                                     cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGA‑ACGACATCACTGGCGGACG‑TACTcc                                                      <  1:211725/251‑1 (MQ=255)                                                                                                                                                                                                gTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGA‑ACGACATCACTGGCGGACG‑TACTCCGGCCTCCTTCGAGCCGTCCATCGACTa                           <  2:143049/251‑1 (MQ=255)                                                                                                                                                                                                                     gAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGA‑ACGACATCACTGGCGGACG‑TACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTc       <  2:54578/250‑1 (MQ=255)                                                                                                                                                                                                                          cGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGCACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTACAGTGGCGGCGAAACTGGCGG‑TGGGGTACAACCTCGGCGAACTGAAGACAGGCCA‑CACTGGCGGACGGAACTCGGGCC‑CCTTCGGGCCGGCCACCGACTATGGGGTTCATATAATTCCTccgttt  >  1:173581/1‑245 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               ATGAGTCGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGG‑TGGGTTACACCCTCGACGAACTGATGA‑ACGACATCACTGGCGGACG‑TACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTC  >  NC_000913/31436‑31902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑