Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,191,644	C→T	E115K (GAA→AAA)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,191,644	0	C	T	96.4%	82.9 / NA	28	E115K (GAA→AAA)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/12);  minor base G (0/1);  total (15/13)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTCAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGGAA  >  NC_000913/1191407‑1191884                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 gtcggtCCATACGGTAGGCCGCGTTTGCCAACTATTTAGCGATGGTTGACTGCGTGGCTGTCTGACCGTGGGTACGAGACAGCAACTGGATAGCGCGATACTGAACTGCGAGATCTTTAATGCCATCAATTAGTGGACGCCAGTATGGCAGGATCAC‑TTCATCACGCTCTGTTTTCAACATTAATACGTTGGATAAGTTATTAATATCTTCCGAAGTACAGGCAAAGGGGATGAATTTAGATACCGCGttc                                                                                                                                                                                                                                     <  2:19098/246‑3 (MQ=255) ggCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGt                                                                                                                                                                                                                                       <  1:177012/249‑1 (MQ=255)   cgcTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGCTACTGCACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCCTCCCGCGAGGTTTTCAGCCTTACTGCGTGGGCGAGGTTCTTGATATCTTCCGAAGTACCGGCAAAGTGGATGAATTTAGAAACcgcgcgc                                                                                                                                                                                                                                     >  2:122272/1‑246 (MQ=255)    gcTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGGGGATGAATTTAGAAACCGCCTGccgt                                                                                                                                                                                                                                  >  2:99625/1‑248 (MQ=255)                                        cGATGGTGGACGGTGTGGCTGGTTGACCGTTGGTACGGGACAGTAGCGGGAGATCGCGATACGGAACGGCGAGAGCTTGAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCGTCCTAATTACAGGCAAAGTGGATGAATGTAGAAACAGCGGCCAGTTCGAGGATCTCCGCCATTTTTTCTGTCAGGAAATAc                                                                                                                                                                                              <  2:13149/251‑1 (MQ=255)                                                                gACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGAT‑ACATTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGttt                                                                                                                                                                      >  1:129459/1‑250 (MQ=255)                                                                   cGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGATATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGGGGATGAATTTAGAAACCGCGTGCAGTGCCGGGATCGCCGCCACTTTTTCTTTCAGGAAATACGCAACCGCGTTAGCGTCGTGGTTAGTg                                                                                                                                                                   <  2:129459/251‑1 (MQ=255)                                                                   cGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTg                                                                                                                                                                   >  1:248113/1‑251 (MQ=255)                                                                                      cGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑GTCATCACGCGCGGTTTTCAGCATTACTGCGTGGGAGAGGTTATTGATATCTTCAGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGCCGTGGTTAGTGGTACGCCCGATAGTTTTg                                                                                                                                                 >  2:132587/1‑250 (MQ=255)                                                                                               tctaTACTGAACGGCGATATCTTTCCTTCCCTCCCTCAGTTGACGCCAGTATGGCAGGATCAC‑CCCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGcgccg                                                                                                                                       >  1:253185/4‑251 (MQ=255)                                                                                               gcgATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGCTAGTTTTGATGCGcgccg                                                                                                                                       >  2:20124/1‑251 (MQ=255)                                                                                                 gATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGcgccgc                                                                                                                                      >  2:91531/1‑250 (MQ=255)                                                                                                    aCTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCAtct                                                                                                                                  <  2:257647/251‑1 (MQ=255)                                                                                                              gagaTCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTg                                                                                                                         >  2:258322/1‑250 (MQ=255)                                                                                                                              tcaatcaGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGc                                                                                                        >  2:81021/1‑251 (MQ=255)                                                                                                                                          cgccagattGGCAGGAGCAC‑TTAATCACGCGCGGTTTTCAGGATTAAGGCGGGGGAGAGGTTATTGAGATCTTCCGAAGTACAGGCAAAGGGGAGGATTTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAAGACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGtgatc                                                                                            <  2:65585/243‑5 (MQ=255)                                                                                                                                                    gCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCg                                                                                  <  1:227967/251‑1 (MQ=255)                                                                                                                                                                          cgGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATGTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCATCCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCTAAAGCAGGATCTTcc                                                             <  1:239628/251‑1 (MQ=255)                                                                                                                                                                                    aaTTAAGGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCtg                                                   <  2:135237/250‑1 (MQ=255)                                                                                                                                                                                          tGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCtgcgtgcg                                             >  2:116535/1‑251 (MQ=255)                                                                                                                                                                                            cGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGcgcg                                           >  2:19403/1‑251 (MQ=255)                                                                                                                                                                                                ggAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGcc                                        >  2:4949/1‑250 (MQ=255)                                                                                                                                                                                                       ttATTGCTATCTTCCGAAGTACAGGCAAAGTGGATGAATTTAGAACCCGCGTGTAGTTCCGGGATCTCCGCCGCTGTTCCTTTCAAGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTTATCGCTGCGTGCGCGGCCAGttttt                                 <  1:268509/250‑1 (MQ=255)                                                                                                                                                                                                                  ttCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAAc                                                                 >  1:186218/1‑207 (MQ=255)                                                                                                                                                                                                                  ttCCGAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAAc                                                                 <  2:186218/207‑1 (MQ=255)                                                                                                                                                                                                                      gAAGTACAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTGTTTGCAGCCAACGTACTTc                 <  1:91531/251‑1 (MQ=255)                                                                                                                                                                                                                            cAGGCAAAGTGGATGAATTTAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTAAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTg           >  2:158906/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggATGAATTGAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGTTAGGGGCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGGCAGTTTTTGCAGCCAACGTACTTCAACTGGTACACGGaa  <  2:253185/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 GGCGCTCCATACGGTAGGCGACGTTTGCCATCTCTTTACCGATGGTTGACGGCGTGGCTGGCTGACCGTGGGTACGAGACAGCAGCGGGATATCGCGATACTGAACGGCGAGATCTTTAATGCCATCAATCAGTTGACGCCAGTATGGCAGGATCAC‑TTCATCACGCGCGGTTTTCAGCATTAATGCGTGGGAGAGGTTATTGATATCTTCCGAAGTACAGGCAAAGTGGATGAATTCAGAAACCGCGTGCAGTTCCGGGATCTCCGCCACTTTTTCTTTCAGGAAATACTCAACCGCTTTAACGTCGTGGTTAGTGGTACGCTCGATAGTTTTGATGCGCGCCGCATCTTCTTCGCTGAAACTGGCGACGATTGCATCAAGGTAACCGATTGCGTCGGCAGCAAAAGCAGGAACTTCCTTGATCGCTGCGTGCGCGGCCAGTTTTTGCAGCCAACGTACTTCAACTTGTACACGGAA  >  NC_000913/1191407‑1191884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑