Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,553,671	C→T	F257F (TTC→TTT)	uxuB →	D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,553,671	0	C	T	100.0%	171.5 / NA	51	F257F (TTC→TTT)	uxuB	D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/20);  total (31/20)

GGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGCCTTTGCCCTGATGATGCAGGAACAAGCGCCA  >  NC_000913/4553426‑4553911                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 gggTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCa                                                                                                                                                                                                                                             <  1:197666/251‑1 (MQ=255)  ggTTGAAAGCGTTTACGGGGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTc                                                                                                                                                                                                                                              >  1:270890/1‑249 (MQ=255)            gTTTACGGTGATGTCCTGCGATAACGTGCGTGCAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGCTTg                                                                                                                                                                                                                                  >  2:252874/1‑251 (MQ=255)            gTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTg                                                                                                                                                                                                                                  >  1:26203/1‑251 (MQ=255)              ttACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGa                                                                                                                                                                                                                                 >  1:64387/1‑250 (MQ=255)                aCGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGa                                                                                                                                                                                                                                 >  1:215941/1‑248 (MQ=255)                  ggTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGAt                                                                                                                                                                                                                             >  2:84772/1‑250 (MQ=255)                    tGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAAc                                                                                                                                                                                                                          >  2:75396/1‑251 (MQ=255)                              cGATAACGTGCGTGAAAACGGTCATGTGGCGCAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCTTTGCCTGCGAACCGTTTCGTCAGTGGGTGGTTGAAGATAACTTCGTTactg                                                                                                                                                                                                                >  2:223667/1‑248 (MQ=255)                                   ccgtgcgtgAAAACGGTGATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCAGGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTctc                                                                                                                                                                                                           <  2:7876/250‑3 (MQ=255)                                           gAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTg                                                                                                                                                                                                   >  1:26791/1‑251 (MQ=255)                                             aaaCGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTggg                                                                                                                                                                                                 <  1:47008/251‑1 (MQ=255)                                                         ggCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCCCCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGgc                                                                                                                                                                                      <  2:170681/250‑1 (MQ=255)                                                           cGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGTGCGca                                                                                                                                                                                   <  1:252874/251‑1 (MQ=255)                                                              aGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGcac                                                                                                                                                                                  >  2:17422/1‑249 (MQ=255)                                                                           ggggCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTGCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGa                                                                                                                                                                     <  1:20586/249‑1 (MQ=255)                                                                            gggCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGCTTGGGATAAAGTGGGCGCCCAGTTCGTTGCAGACGt                                                                                                                                                                  >  2:216516/1‑251 (MQ=255)                                                                              gctggctCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTtg                                                                                                                                                                >  1:277839/1‑251 (MQ=255)                                                                                                cccGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAatgatg                                                                                                                                               >  2:230386/1‑250 (MQ=255)                                                                                                     aGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTg                                                                                                                                         <  2:32684/251‑1 (MQ=255)                                                                                                             gcATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGGGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCt                                                                                                                                 <  1:41401/251‑1 (MQ=255)                                                                                                              cATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTg                                                                                                                                <  2:270890/251‑1 (MQ=255)                                                                                                                      gaagaaAATGTCACCGTCCCGGGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAg                                                                                                                        <  2:191332/251‑1 (MQ=255)                                                                                                                       aagaaAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGc                                                                                                                       >  2:296813/1‑251 (MQ=255)                                                                                                                               gTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCttt                                                                                                               >  2:51692/1‑251 (MQ=255)                                                                                                                                   ccTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTTCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTg                                                                                                           >  2:248655/1‑251 (MQ=255)                                                                                                                                     ttaccGTGCACCACGGTTGACCGCATAGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGATTCGTCCGGGGGGGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATTAAGTGGTCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGCAGCTGCGTATGCTGAACGGCAGCCCCTCTTTTCTggg                                                                                                         >  2:47156/4‑250 (MQ=255)                                                                                                                                             cACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCg                                                                                                 >  1:144364/1‑251 (MQ=255)                                                                                                                                             cACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGAGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCg                                                                                                 >  1:303200/1‑251 (MQ=255)                                                                                                                                              aCCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCgg                                                                                                >  1:26510/1‑251 (MQ=255)                                                                                                                                              aCCATGGTTGACCGAATCGTTCCGGCGGCGCCGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTCCGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAACTGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCgg                                                                                                >  2:204203/1‑251 (MQ=255)                                                                                                                                                 aTGGTTGACAGCATCGTCCCGGCGGCGCCGCCAGACACCTTACAGGAAACAGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCCGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGtt                                                                                              >  2:45558/1‑250 (MQ=255)                                                                                                                                                      tGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTc                                                                                         <  1:51692/250‑1 (MQ=255)                                                                                                                                                         ccGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGCGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAACGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTcggcg                                                                                     >  2:24739/1‑251 (MQ=255)                                                                                                                                                                   ccGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAAc                                                                           >  2:234750/1‑251 (MQ=255)                                                                                                                                                                     ggcggcGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAAcc                                                                          >  2:215144/1‑250 (MQ=255)                                                                                                                                                                        ggcgACGCCAGAAACCTTACAGGAAATCGCTGACCAGCTGGGTGTTTACGAACCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTg                                                                      >  2:305779/1‑251 (MQ=255)                                                                                                                                                                         gcgACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGc                                                                     <  1:51904/251‑1 (MQ=255)                                                                                                                                                                            aCGCCAGAAACCTTACAGGAAATTGCTGACCAGATGGGTGTTTACGACCCGTGCGCCATTGCCGGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGAGAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGa                                                                  <  2:167399/251‑1 (MQ=255)                                                                                                                                                                                  gAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTAAGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATTAAGCTGTGTATGCGGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTTCCTCGGCGGCTATGAAACCATTGCCGACACCGt                                                            <  2:155221/251‑1 (MQ=255)                                                                                                                                                                                         tACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAAc                                                     >  1:236121/1‑251 (MQ=255)                                                                                                                                                                                                 aTTGCTGACCAGCTGGGTTTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATGGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTACAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGTCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTa                                             <  2:210347/251‑1 (MQ=255)                                                                                                                                                                                                         ccAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGCACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTCTCGCaa                                       >  1:137235/1‑249 (MQ=255)                                                                                                                                                                                                           aGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCg                                   >  2:241689/1‑251 (MQ=255)                                                                                                                                                                                                              tGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGcc                                >  1:63944/1‑251 (MQ=255)                                                                                                                                                                                                                 gtgtTTACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGCCttt                             <  2:62224/251‑1 (MQ=255)                                                                                                                                                                                                                 gtggttACGACCCGTGCGCCATTGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTGCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCGGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGCCttt                             <  2:286905/247‑1 (MQ=255)                                                                                                                                                                                                                       aCGACCCGTGCGCCATTGCCTGCGAACCTTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGCCTTTGCCCtg                       <  2:19531/251‑1 (MQ=255)                                                                                                                                                                                                                                       tGCCTGCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCTGCCTTTGCCCTGATGATGCAGGAACaa        <  2:30453/250‑1 (MQ=255)                                                                                                                                                                                                                                            gCGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGCCTTTGCCCTGATGATGCAGGAACAAGCGCCa  <  1:296813/251‑1 (MQ=255)                                                                                                                                                                                                                                             cGAACCGTTTCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGCCTTTGCCCTGATGATGCAGGAACAAGCGCCa  >  1:264461/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 GGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAACGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGATTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATTGCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGTTAATGGTCGCCCGGATTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTATGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCGTGACTAACCCGGCTTATCGCAAAGCGGCCTTTGCCCTGATGATGCAGGAACAAGCGCCA  >  NC_000913/4553426‑4553911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑