Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,530,797	C→T	Q285Q (CAG→CAA)	sgcX ←	putative endoglucanase with Zn‑dependent exopeptidase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,530,797	0	C	T	100.0%	126.6 / NA	40	Q285Q (CAG→CAA)	sgcX	putative endoglucanase with Zn‑dependent exopeptidase domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/16);  total (24/16)

GCCTGAATCAGGCTCAACGGGGAAATGTGCTGCTGACATACCTGCCAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGAT  >  NC_000913/4530557‑4531038                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  gCCTGAATCAGGCTCAACGGGGAAATGTGCTGCTGACATACCTGCCAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAAc                                                                                                                                                                                                                                          >  1:191088/1‑250 (MQ=255) gCCTGAATAAGGCTCAACGGGGAAATGTGCTGCTGACATACCTGCCAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAAc                                                                                                                                                                                                                                          >  2:103208/1‑250 (MQ=255)           ggCTCAACGGGGAAATGTGCTGCTGACATACCTGCCAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGTATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTTTGTTCGAGAGCCGCTTGTTCCAACATCCGTATCAg                                                                                                                                                                                                                               <  1:261371/251‑1 (MQ=255)                     ggAAATGTGCTGCTGACATACCTGCCAGAGCGGTCAATAGGCGGATGCAATCCGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGc                                                                                                                                                                                                                     >  1:25085/1‑251 (MQ=255)                     ggAAATGTGCTGCTGACATACCTGCCAGAGCGATCAATAGGCGGATGCAATAAGTCAAAACACGCAGGCTGGCAACATACGCCGGAGAAAGGGAATAGCGACAAGGAATAGAGAGAGTGGCGCAGGGAATACAATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCAATTCTCGCTGAACTGGAACGTTGTGTTCAAGAGCCGTTTGTTCCAACCTCAGAATCCGGCGAGGGGGc                                                                                                                                                                                                                     >  1:283186/1‑251 (MQ=255)                                   gACATACCTGCCAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGc                                                                                                                                                                                                       <  1:16/251‑1 (MQ=255)                                      aTACCTGCCAGAGCGGTCAATAGGCGGATGCAATCAGTAAAGTCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGCGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGATCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGcc                                                                                                                                                                                                      >  1:294045/1‑249 (MQ=255)                                             ccAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCCGGCTGGCAACATCCGCCGGAGAATGGGTATAGCGACCAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTccc                                                                                                                                                                                             >  1:286811/1‑251 (MQ=255)                                             ccAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTccc                                                                                                                                                                                             >  1:138891/1‑251 (MQ=255)                                                              cGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATTAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTc                                                                                                                                                                              >  1:216405/1‑249 (MQ=255)                                                              cGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTc                                                                                                                                                                              >  1:237617/1‑249 (MQ=255)                                                               agattcAATCAGTCGAATCACACAGTCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGGCTACCATCCTGCTCCATCTGGATATAACCGGTTTCTGTTATCACACCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGAGAGGAGGCGTGATTCATCCGGACAGCGGTCCCCGACCATGATAGTTcagg                                                                                                                                                                           >  2:133743/6‑251 (MQ=255)                                                                   gCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTcaggcagg                                                                                                                                                                       >  2:265160/1‑251 (MQ=255)                                                                          gTCAAATCACGCAGGCTGGCACCCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATccc                                                                                                                                                                <  2:118943/251‑1 (MQ=255)                                                                            cAAATGACGCGGACTGGAAACCTCCGCCGGAGAATGTGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCCACCTGGATATACCCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCaa                                                                                                                                                              <  2:240628/251‑1 (MQ=255)                                                                              aaTCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACg                                                                                                                                                            >  2:253696/1‑251 (MQ=255)                                                                               aTCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGtt                                                                                                                                                                               <  2:72505/231‑1 (MQ=255)                                                                               aTCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGtt                                                                                                                                                                               >  1:72505/1‑231 (MQ=255)                                                                                              aaCCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTg                                                                                                                                             >  1:279417/1‑250 (MQ=255)                                                                                                ccTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGAcc                                                                                                                                          >  1:173586/1‑251 (MQ=255)                                                                                                 cTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCt                                                                                                                                         >  1:262008/1‑251 (MQ=255)                                                                                                            aaTGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCg                                                                                                                              >  2:217763/1‑251 (MQ=255)                                                                                                            aaTGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCg                                                                                                                              <  1:265160/251‑1 (MQ=255)                                                                                                               gggTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCgtg                                                                                                                            <  2:57524/250‑1 (MQ=255)                                                                                                                   ataGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAAt                                                                                                                       >  1:21302/1‑251 (MQ=255)                                                                                                                     aGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCGGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGAGTAATCCTGACCTCGGAATAATCGTGTAAATc                                                                                                                      <  1:14552/250‑1 (MQ=255)                                                                                                                       cGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAgg                                                                                                                   <  1:131707/251‑1 (MQ=255)                                                                                                                                                         cATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATc                                                                                 <  1:204658/251‑1 (MQ=255)                                                                                                                                                                   aaCCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCcaggtcag                                                                       <  2:138891/251‑1 (MQ=255)                                                                                                                                                                     ccTGGTTATAACCGGTTTCTGTTATCACGTCGGGAGCCTCTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTTATATCAATACCAATCGCcaggtcaggt                                                                     <  1:25474/251‑1 (MQ=255)                                                                                                                                                                       tGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCAGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCACCGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATAAATACCAATCGCCAGGTCAGGttt                                                                   >  2:200590/1‑251 (MQ=255)                                                                                                                                                                          atatAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTac                                                                >  2:76802/1‑251 (MQ=255)                                                                                                                                                                                           tATCACGCCGGGAGCCACTTCTCGCTGCACCGGCATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAAt                                                                                                                                                 >  2:303416/1‑153 (MQ=255)                                                                                                                                                                                           tATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGTCGAGGTGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGCAATCAAGACCAATCGCCAGGGCAGGTTTTACACGGCGGAATACGGGa                                                >  2:146450/1‑250 (MQ=255)                                                                                                                                                                                           tATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTTATTAATCCGGCCAGCGTGCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAAt                                                                                                                                                 <  1:303416/153‑1 (MQ=255)                                                                                                                                                                                              cACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACa                                             <  2:242437/250‑1 (MQ=255)                                                                                                                                                                                              cACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACa                                             >  2:67699/1‑250 (MQ=255)                                                                                                                                                                                                                               gttgtGTTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAAGTCTTCCTGTACCGAAg           <  1:67699/251‑1 (MQ=255)                                                                                                                                                                                                                                    gtTCAAGAGCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGccacc      <  2:106982/251‑1 (MQ=255)                                                                                                                                                                                                                                         agagCCGTTTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCATGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGAt  >  1:171935/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  GCCTGAATCAGGCTCAACGGGGAAATGTGCTGCTGACATACCTGCCAGAGCGGTCAATAGGCGGATGCAATCAGTCAAATCACGCAGGCTGGCAACCTCCGCCGGAGAATGGGTATAGCGACAAGGAATAGAGAGACTGGCGCAGGGAATACCATCCTGCTCAACCTGGATATAACCGGTTTCTGTTATCACGCCGGGAGCCACTTCTCGCTGCACCGGAATGTTGTGTTCAAGAGCCGTCTGTTCCAACATCCGTATCAGGCGAGGAGGCGTGATTAATCCGGCCAGCGTTCCCCGACCATGGTAGTTCAGGCAGGTGATCCCAACGCCCTGATTAATCCTGACCTCGGAATAATCGTGTAAATCAGGGGTGTCACACGATGGAGTGATATCAATACCAATCGCCAGGTCAGGTTTTACACGGCGTAATACGGGAACAATGCCGCGGATATTAAATTCTTCCTGTACCGAAGCCACCAGAT  >  NC_000913/4530557‑4531038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑