Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,524,401	C→T	G204S (GGC→AGC)	yjhH ←	putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,524,401	0	C	T	89.7%	92.2 / ‑5.1	40	G204S (GGC→AGC)	yjhH	putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base T (15/20);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 4.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCTTCAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGAC  >  NC_000913/4524157‑4524629                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     gCTTCTTCAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTGTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTcagcag                                                                                                                                                                                                                                <  2:12811/251‑1 (MQ=255)     cttcAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATc                                                                                                                                                                                                                            >  1:192046/1‑251 (MQ=255)      ttcAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGTATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCg                                                                                                                                                                                                                           >  1:186510/1‑251 (MQ=255)                  aaGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAAt                                                                                                                                                                                                               <  1:40700/251‑1 (MQ=255)                               aaGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTATCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAAcc                                                                                                                                                                                                  <  2:266624/251‑1 (MQ=255)                                                aGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGGCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                                                                                                                                                 <  2:268287/251‑1 (MQ=255)                                                aGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                                                                                                                                                 <  2:268297/251‑1 (MQ=255)                                                  gCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGCCGCCGCTCAGCAGCATCGTATTCAGCCAATGATAATCGTAAACGCAGAATACCCACAacgac                                                                                                                                                                               >  2:94636/1‑251 (MQ=255)                                                   cAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacgacg                                                                                                                                                                              <  2:274486/251‑1 (MQ=255)                                                   cAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGGCGCCGCTCAGCCGCCTCGTATTCAGCAAATTGTCATCTTAACCGCCGGATACCGAAAacgacc                                                                                                                                                                              >  2:86215/1‑250 (MQ=255)                                                           cacacTGTATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTAcc                                                                                                                                                                      <  2:186510/251‑1 (MQ=255)                                                               cTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGCTCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCGCAGCAGCCTCGTATTCAGCACATGATCATCGCAACCGCAGAATACCGCAAACGACCGGCGTACCGAcc                                                                                                                                                                  >  2:43006/1‑250 (MQ=255)                                                                     gCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTCCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACtg                                                                                                                                                            <  1:245709/251‑1 (MQ=255)                                                                        gTATTTGATCAGTGAGACAAACTGTGTTTCGAGGGCGTAAATCGCGGGCAGTTGTAGGAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTTCGGAGCAAAGTTAGCGCTGGCGGTTATCGGACCGTCGCCGCTCAGCGGCATCGTATTCATCAAATGATCATCGTAACCGGAGAATACCGAAAACTACGGCCATACCTACTTAAgtgtgt                                                                                                                                                         <  2:107051/251‑1 (MQ=255)                                                                        gTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTgtgt                                                                                                                                                         <  2:237445/251‑1 (MQ=255)                                                                          aTTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTg                                                                                                                                                       >  1:16254/1‑251 (MQ=255)                                                                              gATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTTTCGCACCGGCGCCGCCCAGCAGCATCGTATTCAGCAAATTATCAACGTTACCGCAGAATACCGACAACCAACGGCGTACCGCCTTTACtgtggtggta                                                                                                                                                    >  2:303232/1‑244 (MQ=255)                                                                                    ggagaCAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTAc                                                                                                                                             <  2:194406/250‑1 (MQ=255)                                                                                           aaCGGAGTTTCGAGGGCGTAAATAGCGGGCCGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGGTATCGAACCGGCGCCGCTCAGCAACAATGGATTCAGCAAATGATCATCGTAACCGCAGAAAACCGAAAACGACGGGCATACCAACTTTAATGAGGTGATCATCGTACGccaatg                                                                                                                                      >  2:194336/1‑246 (MQ=255)                                                                                              ggTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCCCTCAGCAGCATTGGATTCAGCCAATGATCATCGTAACCCCAGAATACCGAAAAAAACGGGCGGACCGAATTAACTGTGGTGTTCATCGTACGCAAGTGAcc                                                                                                                                   >  2:241093/1‑251 (MQ=255)                                                                                                 gtTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCCCGCCAGGCGCGGAAGATGCCGACGGAGAGTTCCGGAGCACAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATAATCCTAACCGCAGCAAACCGAAAACCACGGGCGTACCGACTTAACTGTGTTGATCCTCGTACGCAAGTGACCaa                                                                                                                                 >  1:129516/1‑250 (MQ=255)                                                                                                 gtTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCaa                                                                                                                                 <  2:59139/250‑1 (MQ=255)                                                                                                 gtTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAAc                                                                                                                                >  2:139609/1‑251 (MQ=255)                                                                                                 gtTTCGAGGACGTAAATAGCGGCCAGATGTAGTAGTTTTTTATTCAGCGTCGCAGCGGCCGACAGATCGCCTTAACGCTAGGCGCGGTAGATGCCGACGGAGAGTTACGGCGCAAAGTTAGCGCTGGCGATTATCGAACAGGCGCCGCTAAGCACAATCGGCATCCGGAAAAGATCATCGTAACCGCAGAAAAACGAAAACGAAGGGCGGACCGACTTCACTGTGTTGCGCATCGAACACAAGGGACCAAc                                                                                                                                >  2:205707/1‑251 (MQ=255)                                                                                                         ggCGTAACTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCCGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCCCCGTCGCCCCCCACCAGCACCGTATTCAGAAAAAGATCATCCTAAACCCAGAAAACCGAACACGACGGGCGAACCGCCTTAAATGTGTTTATAATCTTACGCAAGTGAcccaagcggtcc                                                                                                                         >  2:276913/1‑240 (MQ=255)                                                                                                              aaaTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCAGCGTATTCAGCAAATGATCATCGGAACCGCAGAATTCCGAAAACGACGGGCGTACCGACTTAACTTTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGgt                                                                                                                    <  1:287032/250‑1 (MQ=255)                                                                                                                            tGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCaa                                                                                                      <  2:159992/250‑1 (MQ=255)                                                                                                                                         tATTCGGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGGACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGtt                                                                                         <  2:284177/250‑1 (MQ=255)                                                                                                                                          aTTCAGCGTCGCAGCGGTCGACAGATCCCCTTCACGCCAGGCGAGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCCGCATCGTATTCAGCAAATGATCATCGTAACCGCCGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCCAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGtttt                                                                                       >  1:232189/1‑251 (MQ=255)                                                                                                                                                            cGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCa                                                                     <  1:163691/251‑1 (MQ=255)                                                                                                                                                             gCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCAc                                                                    <  1:269391/251‑1 (MQ=255)                                                                                                                                                             gCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGGGACCAACGCTGTCGATGGGGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCAc                                                                    >  2:269391/1‑251 (MQ=255)                                                                                                                                                               cAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCCACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACgg                                                                  >  1:49950/1‑251 (MQ=255)                                                                                                                                                                  aTCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGGTGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGttt                                                               >  1:252345/1‑251 (MQ=255)                                                                                                                                                                                aGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGGCTTTGATGCCAACGATATTTTCGTTTTGCAGAGCCAGACGCGTCACGGGTTCCGGGGTTAAGTcc                                                 >  1:9951/1‑251 (MQ=255)                                                                                                                                                                                        tAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCCCCGTCGCCGCCCAGCAGCATCGTATTCAGCCACTGATCATCGTAACCGCAGAATACCGCAAAAGACGGGCGTACCGACTTTACTGTGTTGATCATCGTACGCAAGTGACCAAAGCTGTCGATGGGGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGt                                         >  1:296711/1‑251 (MQ=255)                                                                                                                                                                                             gCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGAt                                    <  2:236192/251‑1 (MQ=255)                                                                                                                                                                                                       agTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGtt                          <  1:139609/251‑1 (MQ=255)                                                                                                                                                                                                             ggAGCAAAGTTAGCGCTGGCGGTTATCGCCCCGTCGCCGCTCAGCCGCATTGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGAGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGGGGCTTTTATGCCCACGATATTCTCGTTTTGCAGAGCCAGACGCGTTACGGTTTCCGGGGGTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTAc                      >  2:55020/1‑249 (MQ=255)                                                                                                                                                                                                                               gCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATTCGGAAAGTTGTACAGGATCACCGGTAGGGTGAc  <  1:128167/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     GCTTCTTCAGATGCTTCAAGAATCGGTGGTAAGCAATATGTCTCTACAGGCAGCCCGACACACTGCATGCTGTATTTGATCAGTGAGACAAACGGTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAGAGCCAGACGCGTCACGGTTTCCGGGGTTAAGTCCTGACCCGTCAGATCCGGAAAGTTGTACAGGATCACCGGTAGGGTGAC  >  NC_000913/4524157‑4524629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑