Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,501,500	C→T	L81L (CTG→TTG)	yjgZ →	uncharacterized protein YjgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,501,500	0	C	T	97.6%	131.5 / NA	41	L81L (CTG→TTG)	yjgZ	uncharacterized protein YjgZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/14);  minor base G (0/1);  total (26/15)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGCTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAACACGCGGGCTGACAATGGGTCAGAATATGAGTTAGCCCGGCGTTATTCCTGGGGTCAGTACTT  >  NC_000913/4501254‑4501744                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACAAACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTCTGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCGCAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTgac                                                                                                                                                                                                                                                  >  1:197102/1‑251 (MQ=255)                  tGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTc                                                                                                                                                                                                                                 >  1:224849/1‑251 (MQ=255)                    gACCGATTATTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGCTAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTg                                                                                                                                                                                                                               >  1:220273/1‑251 (MQ=255)                      ccGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGcc                                                                                                                                                                                                                             <  1:5351/251‑1 (MQ=255)                           tcttGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGt                                                                                                                                                                                                                        >  1:3363/1‑251 (MQ=255)                                tCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCg                                                                                                                                                                                                                   >  1:80413/1‑251 (MQ=255)                                 ccTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAACATCGTGCATTTCAAGGCACACACCACAATCTCTGCGCGCGTGCTATCCGTCCGGTCGTTATGGGACGCCCGGCCTGGTTGTTCGCTGGTTCCCTGGTGGCCGGGAACCGCGCGGCACAGCTACTGAGCCTTCTGGCACCCGCAGTCTGGCGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCgg                                                                                                                                                                                                                  >  1:121681/1‑251 (MQ=255)                                             aGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTgg                                                                                                                                                                                                      <  2:77232/251‑1 (MQ=255)                                                   agTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACCATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGt                                                                                                                                                                                                >  2:61060/1‑251 (MQ=255)                                                                  aCTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAgg                                                                                                                                                                                 <  2:61315/251‑1 (MQ=255)                                                                        aCCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCttt                                                                                                                                                                            >  1:180199/1‑250 (MQ=255)                                                                              ggAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACcttct                                                                                                                                                                     >  1:236363/1‑251 (MQ=255)                                                                                        tGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGCGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAgg                                                                                                                                                           >  2:152880/1‑250 (MQ=255)                                                                                          cATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTgg                                                                                                                                                         >  2:263597/1‑250 (MQ=255)                                                                                                 aGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGATCCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGc                                                                                                                                                  >  2:206885/1‑251 (MQ=255)                                                                                                   gCACACACAACCATATCTGCGAGCGTGCTATCCGTCAGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCCCTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCCCATGCTTGGTTGACGGCCGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTAGGGTAAGTGATTACTGCCg                                                                                                                                                >  2:156385/1‑251 (MQ=255)                                                                                                      cacacaACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTc                                                                                                                                              >  1:73517/1‑250 (MQ=255)                                                                                                        cacaACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAgg                                                                                                                                           <  2:300252/251‑1 (MQ=255)                                                                                                              aaTATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGAc                                                                                                                                                                                                                                      >  1:215304/1‑154 (MQ=255)                                                                                                              aaTATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGAc                                                                                                                                                                                                                                      <  2:215304/154‑1 (MQ=255)                                                                                                               atatCAGCGAGCGTGATATCCGTCCGGTAGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGAAACCGCGCGGCAAAGCTAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGCGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGACTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGGGTTtgg                                                                                                                                    >  2:155512/1‑250 (MQ=255)                                                                                                                       gAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGGGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCggg                                                                                                                            >  2:260913/1‑251 (MQ=255)                                                                                                                                   cgtccgGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCa                                                                                                                <  1:304471/251‑1 (MQ=255)                                                                                                                                               aTGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTg                                                                                                     <  1:101498/250‑1 (MQ=255)                                                                                                                                                              tGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAgg                                                                                     >  1:281837/1‑251 (MQ=255)                                                                                                                                                                gttgttCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGAAAACAGGa                                                                                    >  2:245274/1‑250 (MQ=255)                                                                                                                                                                gttgttCGCTGGTTCACGGGTGGCCGTGAACCGCGCGGCACAGAGAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGaa                                                                                   <  1:35522/251‑1 (MQ=255)                                                                                                                                                                   gttCGCGGGGTCACTGGTGGCCGGGAACCGCGCGGAACCGAGAATGAGCCTGCTGGGAACCAGAGGCTGGAGCGGCATGCTGGGGGGACGGACGTCCTGACGGGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGTTGGACCCTCTCCGGGTAAGTGAGACCTGCCGTCAGGGGGATGGGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGGTTGACAACAGGAAATg                                                                                <  2:154867/251‑1 (MQ=255)                                                                                                                                                                     tCGCTGGTTACCGGGTGGCCGGGAACCGCGGGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCGGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACATGCAGGTGGGAATTGAACGCCTGACAACAGGAAAtta                                                                               <  2:20338/250‑3 (MQ=255)                                                                                                                                                                        cTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCt                                                                            >  2:30369/1‑250 (MQ=255)                                                                                                                                                                            ttCACTGGTGACCGGGAACCGCGAGGCACCGATACTGAGCCTTCTGGGAACCGCCGTCTGGAGCCACATGATTGGTTGACGGACGTCCTGACGCGTCTGCCGGCGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTAAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGc                                                                       >  2:275547/1‑251 (MQ=255)                                                                                                                                                                             tCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGc                                                                       <  1:241284/250‑1 (MQ=255)                                                                                                                                                                                cggttggCCGGGAACCGCGCGGCGCAGATAATGAGCCTTCTGGGAACCGCAGTCGGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCATCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTa                                                                   <  2:197102/247‑1 (MQ=255)                                                                                                                                                                                cTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGt                                                                    >  1:163338/1‑250 (MQ=255)                                                                                                                                                                                     ggCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAaca                                                               <  1:223637/250‑1 (MQ=255)                                                                                                                                                                                         gggAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATGCCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAACACGCgg                                                          <  1:85005/251‑1 (MQ=255)                                                                                                                                                                                             aCCGCGCGGCACCGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAACACGCGGGCTg                                                      >  2:114402/1‑251 (MQ=255)                                                                                                                                                                                                         aGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAACACGCGGGCTGACAATGGGTc                                            >  1:197912/1‑249 (MQ=255)                                                                                                                                                                                                                           gAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAACACGCGGGCTGACAATGGGTCAGAATATGAGTTAGCCCGGc                        >  2:239631/1‑251 (MQ=255)                                                                                                                                                                                                                                                 gCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAACACGCGGGCTGACAATGGGTCAGAATATGAGTTAGCCCGGCGTTATTCCTGGGGTCAGTACt   <  2:224849/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gCTTGGTTGACGGACGTCATGACGCGTCTGCCGGAGTGGCCGGAGGTGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGCATTGAACGCCTGACAACAGGAAATTAGCTAGAGCAGTAAACACGCGGGCTGACAATGGGTCAGAATATGCGTTAGCCCGGCGTTATTCCTGGGGTCAGTACtt  >  1:170255/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GCCCTTTTGTTACCTCCTGGACCGCTTCTTGTCCTTCCAGTCGGAGCGAGAGTTAGCCGGGGCGGACTTTCACCGACGGAAAGATCGTGCATTTCAAGGCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGCTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGAACGCCTGACAACAGGAAATGAGCTAGAGCAGTAAACACGCGGGCTGACAATGGGTCAGAATATGAGTTAGCCCGGCGTTATTCCTGGGGTCAGTACTT  >  NC_000913/4501254‑4501744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑