Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,487,022	C→T	R269C (CGT→TGT)	lptF →	lipopolysaccharide transport system protein LptF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,487,022	0	C	T	100.0%	173.8 / NA	53	R269C (CGT→TGT)	lptF	lipopolysaccharide transport system protein LptF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/30);  total (23/30)

GGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGG‑ACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACC  >  NC_000913/4486778‑4487267                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ggtggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATc                                                                                                                                                                                                                                                   >  2:125884/1‑250 (MQ=255)  gtggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCAc                                                                                                                                                                                                                                                 >  2:114468/1‑251 (MQ=255)    ggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGt                                                                                                                                                                                                                                               <  1:84225/251‑1 (MQ=255)       ggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGTGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTg                                                                                                                                                                                                                                             <  1:35394/250‑1 (MQ=255)        gCCGATTCCGGACATGTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGGGGAACACTGACACCGAGCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGt                                                                                                                                                                                                                                           <  2:255801/251‑1 (MQ=255)         ccGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGt                                                                                                                                                                                                                                           >  2:277355/1‑250 (MQ=255)         ccGATGCCGGACAATTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTAGCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGCCCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTa                                                                                                                                                                                                                                          <  2:162011/251‑1 (MQ=255)           gagtCCGGACATTTAACCCAGCTGCGCGACGGCGCCCAGGGCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCAGTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGTGCACATTGTGGAAAACTTACACCGATCGTGCTCGCGCAGAACTGAACGGGTGTATctcggttgtatt                                                                                                                                                                                                                                        <  2:83525/248‑12 (MQ=255)            aTTCCGGACATTTAACCCAGCTGAGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTc                                                                                                                                                                                                                                       >  2:6790/1‑251 (MQ=255)              tCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACGCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATAGCGACCAGATGGACATGCGCACATTGTGGGACACGGACTCCGATCGTGCCCGCTCAGAACTGAACTGGTGCATCGCTTTGGTATTCa                                                                                                                                                                                                                                      <  2:284871/250‑1 (MQ=255)                   aCATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCgtgt                                                                                                                                                                                                                                >  1:120906/1‑251 (MQ=255)                         aaCCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTatga                                                                                                                                                                                                                          <  2:174511/251‑1 (MQ=255)                           cccAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTatgatg                                                                                                                                                                                                                        <  1:232414/251‑1 (MQ=255)                           cccAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTatgatg                                                                                                                                                                                                                        <  1:171198/251‑1 (MQ=255)                                  gcgcGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACtt                                                                                                                                                                                                                  <  1:145288/250‑1 (MQ=255)                                      gACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGt                                                                                                                                                                                                             <  1:150295/251‑1 (MQ=255)                                             cccAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCCCGTTGGAATTCACCGTGTTTATGATGGCACTTATGGTCGTAcc                                                                                                                                                                                                       <  1:232758/250‑1 (MQ=255)                                                aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTg                                                                                                                                                                                                   <  2:128647/251‑1 (MQ=255)                                                         ctctCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGtt                                                                                                                                                                                          >  1:205904/1‑251 (MQ=255)                                                              aaCCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAAccc                                                                                                                                                                                     <  2:58265/251‑1 (MQ=255)                                                                 cAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACg                                                                                                                                                                                  >  1:168264/1‑251 (MQ=255)                                                                  aGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGt                                                                                                                                                                                 <  2:67480/251‑1 (MQ=255)                                                                               cGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTAc                                                                                                                                                                    <  1:142718/251‑1 (MQ=255)                                                                                gAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACt                                                                                                                                                                   >  2:59065/1‑251 (MQ=255)                                                                                  aGGCACTGCAATGTTACGTGATTTACGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACTTTGGGATTCGCCGTGTTTATGATGGCACTTATGGTCGTACCTCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGt                                                                                                                                                                 <  2:129167/251‑1 (MQ=255)                                                                                        tGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGAtgc                                                                                                                                                           >  2:229550/1‑251 (MQ=255)                                                                                        tGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGAtgc                                                                                                                                                           >  2:164652/1‑251 (MQ=255)                                                                                                     gATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCAtgct                                                                                                                                              <  2:214085/251‑1 (MQ=255)                                                                                                     gATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCAtgct                                                                                                                                              <  2:104823/251‑1 (MQ=255)                                                                                                        ttCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTTCCAGCCAtgctgc                                                                                                                                            <  2:171398/250‑1 (MQ=255)                                                                                                           cGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGt                                                                                                                                         >  1:97697/1‑250 (MQ=255)                                                                                                              agtACGGACTTCCAGGATTATCAGGCGATCATTGTTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCGCATTGTGGAACACTTACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGTACGCGTACTGTCGATGCTGCCAGCCATGCTGCGGTATCt                                                                                                                                     <  2:269374/249‑1 (MQ=255)                                                                                                                    gACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGGACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACtttt                                                                                                                               <  1:188582/251‑1 (MQ=255)                                                                                                                     aCTTCCAGGATGATCAGGCGATCATTGGTCACCCGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTAGAACTCTGACTCCGATCGGGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCTCCGTGTTTATGAGGGTACTTATGGTCGTACCGCTGAGCGTGGTTAACCCGCTTCAGGGACGCGTACTGTCGATGCTGCCAGCCATgctgcttgttttacttttc                                                                                                                              <  2:31539/251‑14 (MQ=255)                                                                                                                        tCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTttcttc                                                                                                                           >  2:148245/1‑251 (MQ=255)                                                                                                                          cAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCACTCGACCCGCACGATACCGACCAGACGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGCCGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCt                                                                                                                         >  2:289035/1‑251 (MQ=255)                                                                                                                           aGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTg                                                                                                                        >  2:21474/1‑251 (MQ=255)                                                                                                                                  tCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGGCACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATcc                                                                                                                    >  2:303595/1‑248 (MQ=255)                                                                                                                                     ggCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGCACGATACCGACCAGATGGACCTGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCt                                                                                                              >  2:278536/1‑251 (MQ=255)                                                                                                                                                gTCACCAGGCGGTGGGGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCg                                                                                                   >  1:235791/1‑251 (MQ=255)                                                                                                                                                  cACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGTAACACTGACTCCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTGTGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGat                                                                                                 <  2:205916/251‑2 (MQ=255)                                                                                                                                                      aGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAAcggc                                                                                             >  1:264180/1‑251 (MQ=255)                                                                                                                                                                       ccGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGa                                                                            <  1:295936/251‑1 (MQ=255)                                                                                                                                                                          aaCGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGGccc                                                                         >  2:871/1‑251 (MQ=255)                                                                                                                                                                              aTACCGACCAGATGGACATGCGCACATTGTGGACCACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCTCTGAAATCGAACGGCGGTAAAAGTAAGCTGGACCCGACg                                                                     >  1:279818/1‑251 (MQ=255)                                                                                                                                                                                cccGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTCCCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCt                                                                   <  1:114468/250‑1 (MQ=255)                                                                                                                                                                                                              aaCACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGG‑ACCGTTAACCTGATTTATCt                                    <  1:160118/251‑1 (MQ=255)                                                                                                                                                                                                                      cacCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGTTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGGTGGAACCGGAGCTGGGGA‑GTGGGACCCGTAACCTGATTTATATGGCttttg                            >  2:213008/1‑249 (MQ=255)                                                                                                                                                                                                                                gcccgcgCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATACTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGG‑ACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTtc                   <  2:208465/247‑1 (MQ=255)                                                                                                                                                                                                                                    gcgcAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGCCCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGG‑ACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAAc              <  2:66195/251‑1 (MQ=255)                                                                                                                                                                                                                                       cAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGGAAAGGGAAGCTGGACCCGACGCCGTGGATGTGG‑ACCGTTAACCCGATTTATTTGGCTTTTGCGGATGTTTTCAACCtt           >  2:262871/1‑251 (MQ=255)                                                                                                                                                                                                                                            ctgaactgGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGG‑ACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTggg       >  1:278214/1‑250 (MQ=255)                                                                                                                                                                                                                                                actgGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGG‑ACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACAcc  <  1:148245/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GGTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGG‑ACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTTTGGGACACC  >  NC_000913/4486778‑4487267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑