Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,901	C→T	V313M (GTG→ATG)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,901	0	C	T	100.0%	144.4 / NA	42	V313M (GTG→ATG)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

CAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACG  >  NC_000913/4482655‑4483142                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  cacccgccgGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTgg                                                                                                                                                                                                                                               <  2:64027/248‑1 (MQ=255)   aCCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGACCGGTCAGCACGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTTGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACTTCTGGgc                                                                                                                                                                                                                                             >  2:227332/1‑251 (MQ=255)         ggTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTc                                                                                                                                                                                                                                       <  1:208520/251‑1 (MQ=255)           tttCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCAc                                                                                                                                                                                                                                     >  1:151967/1‑251 (MQ=255)               gCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGat                                                                                                                                                                                                                                 <  2:237143/251‑1 (MQ=255)                     aCATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGACCATCTGGGCGCTTTCACGGATATCGcc                                                                                                                                                                                                                           >  2:178048/1‑251 (MQ=255)                           gcacgcccGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCaaa                                                                                                                                                                                                                     <  2:48096/251‑1 (MQ=255)                                   gTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCCGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAgg                                                                                                                                                                                                              >  2:150824/1‑250 (MQ=255)                                     aCCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGTAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATg                                                                                                                                                                                                           >  2:250057/1‑251 (MQ=255)                                       cACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGtt                                                                                                                                                                                                         <  1:151726/251‑1 (MQ=255)                                           ggtcggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCTGGCAAAGGTCAGGATGTTGatc                                                                                                                                                                                                     <  2:182732/251‑1 (MQ=255)                                               ggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCg                                                                                                                                                                                                 >  1:110581/1‑251 (MQ=255)                                                     cATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAggg                                                                                                                                                                                           >  2:289915/1‑251 (MQ=255)                                                                       cccgccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACtt                                                                                                                                                                         <  2:9573/250‑1 (MQ=255)                                                                             aCGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAgg                                                                                                                                                                   >  2:171367/1‑250 (MQ=255)                                                                             aCGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAgg                                                                                                                                                                   >  2:171359/1‑250 (MQ=255)                                                                               ggTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGTGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCg                                                                                                                                                                 <  2:151967/251‑1 (MQ=255)                                                                                      gTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAg                                                                                                                                                                                                                    <  2:96131/193‑1 (MQ=255)                                                                                      gTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAg                                                                                                                                                                                                                    >  1:96131/1‑193 (MQ=255)                                                                                           gAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTgc                                                                                                                                                     <  1:254954/251‑1 (MQ=255)                                                                                                           cGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTc                                                                                                                                      >  1:303326/1‑250 (MQ=255)                                                                                                                    ttcttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCa                                                                                                                                                                            >  1:277580/1‑203 (MQ=255)                                                                                                                    ttcttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCg                                                                                                                             <  2:281977/250‑1 (MQ=255)                                                                                                                    ttcttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAAGAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGTCGTGACGTGTACCCa                                                                                                                                                                            <  2:277580/203‑1 (MQ=255)                                                                                                                     tcttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGt                                                                                                                           >  2:248526/1‑251 (MQ=255)                                                                                                                                gCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTcc                                                                                                                 >  2:224727/1‑250 (MQ=255)                                                                                                                                  cAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGGGATTTTCACGCATACGGTGCCTTTTTccttg                                                                                                              >  2:253656/1‑248 (MQ=255)                                                                                                                                    aGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTc                                                                                                            >  1:205099/1‑251 (MQ=255)                                                                                                                                        cgTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAACCGCTCCAGGTTCTGGAACTCTGCAGTGATTTCGCTGGAATAACCGTCAGATGCGTTACCTTTGGTATCGAACATCTGGGCGCGTTCACGGATATCTCCGTCAAAGGTCAGGATGTTGATCATCGGCGGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGTAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCg                                                                                                        <  1:246850/251‑1 (MQ=255)                                                                                                                                                gCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTttgtc                                                                                                <  1:171367/251‑1 (MQ=255)                                                                                                                                                gCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTtcgtc                                                                                                <  1:171359/251‑1 (MQ=255)                                                                                                                                                   gCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGcc                                                                                             >  1:212038/1‑251 (MQ=255)                                                                                                                                                                                      tttCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGa                                                           >  1:139438/1‑250 (MQ=255)                                                                                                                                                                                        tCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAAt                                                         <  1:216392/250‑1 (MQ=255)                                                                                                                                                                                                   gCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGcc                                             <  2:303326/251‑1 (MQ=255)                                                                                                                                                                                                   gCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGATTTCACGGATATAGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGAAGTTTACCAACTTCCTAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGAACGGAATACGACGGTTCACCAGCGGCAGAATGACATCttttcc                                             >  2:109977/1‑248 (MQ=255)                                                                                                                                                                                                        gATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCa                                        <  2:118244/251‑1 (MQ=255)                                                                                                                                                                                                           ttCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTTCCAATCAGa                                      <  1:219278/250‑1 (MQ=255)                                                                                                                                                                                                                        aCGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGATTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCgg                        >  1:274432/1‑251 (MQ=255)                                                                                                                                                                                                                                  cGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCggg              <  2:110581/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGGGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAAc         >  2:159878/1‑251 (MQ=255)                                                                                                                                                                                                                                              tATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCGACg  >  2:71419/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CAACCGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTTAACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACG  >  NC_000913/4482655‑4483142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑