Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,451,280	C→T	P75S (CCG→TCG)	ytfR →	galactofuranose ABC transporter putative ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,451,280	0	C	T	97.2%	112.7 / ‑5.5	36	P75S (CCG→TCG)	ytfR	galactofuranose ABC transporter putative ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/17);  minor base G (1/0);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGC  >  NC_000913/4451038‑4451521                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   gCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCATCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAAt                                                                                                                                                                                                                                            <  2:130597/251‑1 (MQ=255)       cacCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGcc                                                                                                                                                                                                                                      >  2:111334/1‑251 (MQ=255)              ggAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACgcgc                                                                                                                                                                                                                               >  1:306516/1‑251 (MQ=255)                    cATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCaacaac                                                                                                                                                                                                                         >  1:246016/1‑251 (MQ=255)                     aTGAAGACCGACCAACACCAGGAGATCCTCCGCACCTAAGGATTAAGTAAATTTTTCCCCGGCGTAAAAGCGTTAGACAACGTTGATGTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGAGCGCGGCACCATCTGGCTGGAAGGCCAGGCGATCTCATCGAAAAATACCGCCCACGCGCAACGACt                                                                                                                                                                                                                        <  2:273756/251‑1 (MQ=255)                              gaccAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCCACGCTAATCAACGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCACCAACTCGGCATTgg                                                                                                                                                                                                               >  2:140416/1‑251 (MQ=255)                                                   cGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCa                                                                                                                                                                                          >  1:181750/1‑250 (MQ=255)                                                                        tttttcaacGGCGTCAAAGCGTTAGACAACGTTGCTTTCAGCCTGCGCCGTGGCGAAATCATGACGCTGCTCGGTGAAAACGGGGCGGGCAAACCAACGCTAATCACAGCATTAACTGGGGTATACCACCCCGCTCCCCGCACCATCTGGCTGGAAGGCCAGGCTCTCTCATCGAAAAATACCGCCCACCCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAAACTGCTACCCAATATgtcggg                                                                                                                                                                    >  2:49263/9‑250 (MQ=255)                                                                                        aaGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCTCCATCTGGCTGGAAGGCCAGGCGATCTCATCGAAAAATACCGCCCACGCGCAACAACACGGCATTGGCAC‑CGTCTATCAGGAAGTCATCCTGCTACCCAATATGTCGGTCGCTGATAATCTAttt                                                                                                                                                    <  1:277728/251‑1 (MQ=255)                                                                                         aGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTg                                                                                                                                                                                                                >  1:59331/1‑191 (MQ=255)                                                                                         aGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTg                                                                                                                                                                                                                <  2:59331/191‑1 (MQ=255)                                                                                                  cAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCcgcg                                                                                                                                          <  1:57800/251‑1 (MQ=255)                                                                                                         gATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCa                                                                                                                                    <  1:189826/250‑1 (MQ=255)                                                                                                               aGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCtt                                                                                                                             <  2:290459/251‑1 (MQ=255)                                                                                                               aGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCtt                                                                                                                             <  2:237987/251‑1 (MQ=255)                                                                                                                           ggCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTAc                                                                                                                  <  1:272090/250‑1 (MQ=255)                                                                                                                           ggCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTAc                                                                                                                  <  2:168355/250‑1 (MQ=255)                                                                                                                               aaaTCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCCTTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTAGGTCCCTGATAATCTATTTATAGGCCGCGCACCCAAACGCTTCGGCCTTCTAcgccgc                                                                                                             >  2:255422/1‑251 (MQ=255)                                                                                                                                   ccaggcggttctCGGTGAAAACGGGGGGGGAAAAACCACCCTAATCAAAGCATTAAATGGGGTATTCCCCGCCCATCCCGGCCCCATCTTGGTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAag                                                                                                         <  1:73454/241‑1 (MQ=255)                                                                                                                                                   gAAAACGGGGCGGGAAAATCAACGCTAAACAAAGCATGAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGTCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCTCTGATAATCTATTTAGAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGTAAAAgcgcg                                                                                          <  2:221646/250‑1 (MQ=255)                                                                                                                                                          gggCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTTCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCTCGCCACCGaa                                                                                  <  2:274141/251‑1 (MQ=255)                                                                                                                                                          gggCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGaa                                                                                  <  1:167485/251‑1 (MQ=255)                                                                                                                                                                  aaaTCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGc                                                                          >  2:97262/1‑251 (MQ=255)                                                                                                                                                                        aCGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCtt                                                                     >  2:58983/1‑250 (MQ=255)                                                                                                                                                                         cGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTAc                                                                   >  2:234579/1‑251 (MQ=255)                                                                                                                                                                          gCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACg                                                                  >  1:175697/1‑251 (MQ=255)                                                                                                                                                                             aaTCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGtt                                                               >  1:157880/1‑251 (MQ=255)                                                                                                                                                                                  aaGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTcc                                                          >  1:123227/1‑251 (MQ=255)                                                                                                                                                                                      aTTAACTGGAGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGCACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCg                                                      >  2:105713/1‑251 (MQ=255)                                                                                                                                                                                                gtATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCCTACGGTTTCTCCCTCGACGTGcgcg                                             <  1:163481/250‑1 (MQ=255)                                                                                                                                                                                                   taCCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAAcc                                         >  1:45326/1‑251 (MQ=255)                                                                                                                                                                                                                  ggCACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTc                          >  2:129308/1‑251 (MQ=255)                                                                                                                                                                                                                    cACCATCTGGCTGGAAGGCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTc                          >  2:231903/1‑249 (MQ=255)                                                                                                                                                                                                                         tCTGGCTGGAAGGCCAGGCTATCTCAGCGAAAAATACCGCCCACGCGGAACAACTCGGCATTGG‑ACACGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATATATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCAAGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCTCGAAACGCTCAACCGCTTTTCAGTcgcg                   >  2:27120/1‑251 (MQ=255)                                                                                                                                                                                                                                     gCCAGGCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtc       <  1:9447/251‑1 (MQ=255)                                                                                                                                                                                                                                          gCTATCTCATCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGc  <  2:66895/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   GCTTTACACCGGGGGAAACCATGACGACCGACCAACACCAGGAGATCCTCCGCACCGAAGGATTAAGTAAATTTTTCCCCGGCGTCAAAGCGTTAGACAACGTTGATTTCAGCCTGCGCCGTGGCGAAATCATGGCGCTGCTCGGTGAAAACGGGGCGGGAAAATCAACGCTAATCAAAGCATTAACTGGTGTATACCACGCCGATCGCGGCACCATCTGGCTGGAAGGCCAGGCTATCTCACCGAAAAATACCGCCCACGCGCAACAACTCGGCATTGGCAC‑CGTCTATCAGGAAGTCAACCTGCTACCCAATATGTCGGTCGCTGATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGC  >  NC_000913/4451038‑4451521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑