Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,196	C→T	P135S (CCG→TCG)	yceD →	DUF177 domain‑containing protein YceD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,196	0	C	T	94.1%	45.8 / NA	17	P135S (CCG→TCG)	yceD	DUF177 domain‑containing protein YceD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/10);  minor base G (0/1);  total (6/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTC  >  NC_000913/1146949‑1147441                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tATCGATAACCAACGTCTCTCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCgg                                                                                                                                                                                                                                                    >  1:81499/1‑251 (MQ=255)         aCCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATgct                                                                                                                                                                                                                                            <  1:146273/251‑3 (MQ=255)                                                    aaCGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTg                                                                                                                                                                                                 >  1:233756/1‑251 (MQ=255)                                                           gAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGcc                                                                                                                                                                                          <  1:225520/251‑1 (MQ=255)                                                               gtCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTga                                                                                                                                                                                       <  1:159574/250‑1 (MQ=255)                                                                             aaGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGcc                                                                                                                                                                        <  2:103343/251‑1 (MQ=255)                                                                                    ttACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAAccc                                                                                                                                                                  >  1:25263/1‑250 (MQ=255)                                                                                          atcaGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTTAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGcc                                                                                                                                                           <  1:148967/251‑1 (MQ=255)                                                                                                               gTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGTCTTGCCGGTAGTTTCGGTGCATGAGTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATGAGCCAGCTTAAAGCgt                                                                                                                                      <  2:226410/251‑1 (MQ=255)                                                                                                                              gTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGGTTCTGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGTCATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCAGTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGcgc                                                                                                                       <  1:169592/251‑3 (MQ=255)                                                                                                                                 cAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTcccc                                                                                                                    <  1:280762/251‑1 (MQ=255)                                                                                                                                                                gTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGCTCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTgagg                                                                                       >  2:195318/1‑249 (MQ=255)                                                                                                                                                                                           cgcggaagtcgttCTGCTTGTAATGGTTGAAGATGAAATCTTCCTCGCCGTGCCGGTAGTTGCGGTGCTTGTTTTTGAACAGTGTGAATTGTCGGAAGCGGACATGTTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTAGTAGCCAGCTTAAAGCGTAAGTAAGTGGTGCTCCCCGTTGGATCGGGGATAAACATTAATTGAGGAGTTAGTTCCATTGCCGTTCAACAGAAt                                                           <  2:255201/238‑1 (MQ=255)                                                                                                                                                                                            ggTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGGTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTTAAGAATCGCATAAGAATAGCCCATTTGCCGGATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGTGAGAATCCGTAATTGAGGAGTAAGGGCCATGGCCGTACAACAGaagaa                                                         <  2:20818/251‑4 (MQ=255)                                                                                                                                                                                                     gATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTTCGGCGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCg                                                >  1:164806/1‑251 (MQ=255)                                                                                                                                                                                                                                    tcCTCGCCTTGCCGGTAGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGAc                 >  1:292103/1‑251 (MQ=255)                                                                                                                                                                                                                                                    aGTTTCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTc  <  2:25263/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAACGAATTCGGTGAAATCGATCTGCTTGCAATGGTTGAAGATGAAATCATCCTCGCCTTGCCGGTAGTTCCGGTGCATGATTCTGAACACTGTGAAGTGTCCGAAGCGGACATGGTCTTTGGTGAACTGCCTGAAGAAGCGCAAAAGCCAAACCCATTTGCCGTATTAGCCAGCTTAAAGCGTAAGTAATTGGTGCTCCCCGTTGGATCGGGGATAAACCGTAATTGAGGAGTAAGGTCCATGGCCGTACAACAGAATAAACCAACCCGTTCCAAACGTGGCATGCGTCGTTCCCATGACGCGCTGACCGCAGTC  >  NC_000913/1146949‑1147441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑