Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,364,798	C→T	G82D (GGC→GAC)	dsbD ←	thiol‑disulfide exchange protein DsbD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,364,798	0	C	T	97.5%	131.9 / NA	40	G82D (GGC→GAC)	dsbD	thiol‑disulfide exchange protein DsbD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/30);  minor base A (1/0);  total (10/30)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGGGCTGCTCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGCCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCAT  >  NC_000913/4364562‑4365042                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     cgGTGGGCTGCTCTTGCGGCGGAACAGACGCAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGGAGGTGACAGTTAACGTCGCTCCCGCAGTCGCCGGGTTGATGGGGACGGGAAGCGTCAGTCGATCGCGGTAAATCTTGCTTTTGCCGTGAATCGCTTCTTCATGCCAGACGTCTTGCGGCAGCTGc                                                                                                                                                                                                                                        <  1:134372/251‑1 (MQ=255)   gTGGGCTGCTCTGGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCAc                                                                                                                                                                                                                                      <  2:164305/251‑1 (MQ=255)     gggCTGCTCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGCCCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGGTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAACCTCATCTTCATGCCCGACGACTTGCGGCAGCTTCACgg                                                                                                                                                                                                                                    >  2:134968/1‑250 (MQ=255)          gctCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCgg                                                                                                                                                                                                                                 <  2:117664/249‑1 (MQ=255)               tgctgcGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAAttt                                                                                                                                                                                                                           <  1:257653/250‑1 (MQ=255)                  tgcGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTcgc                                                                                                                                                                                                                       >  2:57625/1‑251 (MQ=255)                   gctgAACAGACACAGGCTGTGGCGCGGCGTTGTTGGCGCCCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTATCGTCGCTCCCGTACGCGCCGGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCCGACGTCTTTCGGCAGCTTCACGTGGGAAATTTTct                                                                                                                                                                                                                        <  2:174137/246‑2 (MQ=255)                                 ggCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTGAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGGTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAAGCTCGCTTTTGCCGTAAGACTCATCTTTATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAAt                                                                                                                                                                                                        <  1:25077/251‑1 (MQ=255)                                     gtgGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGc                                                                                                                                                                                                      <  1:158569/249‑1 (MQ=255)                                            tGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGtt                                                                                                                                                                                             >  2:56564/1‑251 (MQ=255)                                                  gttgGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGt                                                                                                                                                                                       <  1:141507/251‑1 (MQ=255)                                                        gatcACTCCGCTTAACGGAACGGTTTTGGTTTCTGGCTGATAACAGAACCCGGCATCTGCTCAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACGCGCCTGGTTGATGGTGACTGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTTCCGTAAAACTCACAATCATGTCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCATGTTCCGGCGTAATTCGGATCTGTTTTCGGTAGAGgt                                                                                                                                                                                 <  2:153463/248‑1 (MQ=255)                                                               tCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGTCGGGAAGCGTCAGCCGATCGCGGGAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAAc                                                                                                                                                                           <  2:289790/250‑1 (MQ=255)                                                                      acggaacggTTTTGTGTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCGGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTTAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTg                                                                                                                                                                   <  2:71367/251‑1 (MQ=255)                                                                       cggaacggTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTg                                                                                                                                                                   >  2:819/1‑250 (MQ=255)                                                                           acggTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTg                                                                                                                                                              <  1:176524/251‑1 (MQ=255)                                                                                      tgcTGGCGGATAGCAGACACCGTCTTCAGCACGGCCGTTGTAGGTGACAGGTTGCGTCGCTCCCGCACTCGCCTGGTTGAAGGTGGCGTGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTGGCCGTAAAACTCATCTTCATGCCAGACGTGTTTCGTCAGCTGCACGTCGGCGATGTTCCCGTGTTGCGGCGGAAGGCGGGTCTGTTTTCGGTAGGGGGAGTATCCGGCTTGTATGTGCCAGTTCAGa                                                                                                                                                    <  2:297474/248‑1 (MQ=255)                                                                                          ggCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTaa                                                                                                                                                <  1:186509/250‑1 (MQ=255)                                                                                          ggCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTa                                                                                                                                                 <  2:231600/249‑1 (MQ=255)                                                                                          ggCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAg                                                                                                                                               <  1:119069/251‑1 (MQ=255)                                                                                                 aaCAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGt                                                                                                                                        >  2:218465/1‑251 (MQ=255)                                                                                                         ccGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACGCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGTTAGATGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTg                                                                                                                                <  1:33758/251‑1 (MQ=255)                                                                                                                                               cGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACa                                                                                           <  1:192035/250‑1 (MQ=255)                                                                                                                                                             ccTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTccc                                                                            <  2:282579/251‑1 (MQ=255)                                                                                                                                                             ccTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCAGCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTccc                                                                            <  2:178712/251‑1 (MQ=255)                                                                                                                                                                ggTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCg                                                                           <  1:265828/249‑1 (MQ=255)                                                                                                                                                                       ggTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCCGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCg                                                                   >  1:15852/1‑250 (MQ=255)                                                                                                                                                                        gTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGaa                                                                 <  1:819/251‑1 (MQ=255)                                                                                                                                                                                    gTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTgg                                                                                                                                     <  2:161682/171‑1 (MQ=255)                                                                                                                                                                                    gTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTgg                                                                                                                                     >  1:161682/1‑171 (MQ=255)                                                                                                                                                                                        gCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCGAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACg                                                 >  1:199359/1‑251 (MQ=255)                                                                                                                                                                                              cgcgGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGTGCGTCGAATAATCCGGCAAAAACGGAAGt                                            <  2:15852/250‑1 (MQ=255)                                                                                                                                                                                              cgcgGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGt                                            <  2:177020/250‑1 (MQ=255)                                                                                                                                                                                                          cGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGc                               <  1:57625/251‑1 (MQ=255)                                                                                                                                                                                                                        aaCTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAAGCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATGCTGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTaaa                 <  1:198787/251‑1 (MQ=255)                                                                                                                                                                                                                         aCTCATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAg                <  1:297845/251‑1 (MQ=255)                                                                                                                                                                                                                            cATCTTCATGCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGAt              <  1:290682/250‑1 (MQ=255)                                                                                                                                                                                                                                     gCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGcc    >  1:45460/1‑251 (MQ=255)                                                                                                                                                                                                                                     gCCAGACGTCTTGCGGCAGCTGCACGTCGGCAATTGTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAAGCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGc     <  1:131485/250‑1 (MQ=255)                                                                                                                                                                                                                                       cAGACGTCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCAt  >  1:175282/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     CGGTGGGCTGCTCTTGCTGCGGAACAGACACAGGCTGTGGCGCTGCGTTGTTGGCGACCACTTCGCTTAACGGAACGGTTTTGGTTTCTGGCGGATAACAGAAACCGGCATCAGCACAGCCCTGGTAGGTGACAGTTAACGTCGCTCCCGCACTCGCCTGGTTGATGGTGACGGGAAGCGTCAGCCGATCGCGGTAAATCTCGCTTTTGCCGTAAAACTCATCTTCATGCCAGACGCCTTGCGGCAGCTGCACGTCGGCAATTTTCGCGTGTTCCGGCGTAATGCGGATCTGTTTACGGTAGAGGTAGTAACCGTCTTTGATCTGCCAGGTCAGATTAAGGTCATGTTGGTTTTGCTGAAAATCAAAAGCAAAGGCTTGATCCGCGGGGACAAATTGTGAACGTCCCGGCGCGTCGAATAATCCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCAT  >  NC_000913/4364562‑4365042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑