Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,322,605	C→T	intergenic (‑183/+731)	phnF ← / ← phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,322,605	0	C	T	100.0%	149.1 / NA	44	intergenic (‑183/+731)	phnF/phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/20);  total (24/20)

CAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCA  >  NC_000913/4322359‑4322851                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        cAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGGCGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGcc                                                                                                                                                                                                                                                      <  1:212996/250‑1 (MQ=255)         gCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTAGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGGGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACg                                                                                                                                                                                                                                             <  1:251365/251‑1 (MQ=255)                     tGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGTAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCg                                                                                                                                                                                                                                    <  1:199442/248‑1 (MQ=255)                     tGATAGCGTGTTGGGTAGCTGGTCGGAGGTGTAGACAAGTGCATAGATATCAAGGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACGGGAAACCGGGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGaa                                                                                                                                                                                                                                  <  2:44324/250‑1 (MQ=255)                           cGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATCTCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGAt                                                                                                                                                                                                                             >  1:273446/1‑249 (MQ=255)                           cGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATag                                                                                                                                                                                                                           <  1:172536/251‑1 (MQ=255)                              gtTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGGGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGGAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATagag                                                                                                                                                                                                                         <  2:264232/250‑1 (MQ=255)                                   gTAGCGGGTCGGATTTGTAGACAAGTGCATAGATACCAATGCCTCGCTTATCAGATAAAGTGCTTCCGCAACCGTTGAGAGAGGAAATCCAGCAGGCGGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACGGGGTGCCGCGGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTa                                                                                                                                                                                                                   <  2:299844/251‑1 (MQ=255)                                         ggTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGTTTTGTTGGAACTGGAACCCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGgaga                                                                                                                                                                                                              <  1:131007/250‑1 (MQ=255)                                             ggATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATcagc                                                                                                                                                                                                         >  1:198646/1‑251 (MQ=255)                                                      gACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATGGAGGGGGTAGGAGATCAGCAGTGGCATc                                                                                                                                                                                                >  2:297015/1‑251 (MQ=255)                                                       aCAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCa                                                                                                                                                                                               <  1:233546/251‑1 (MQ=255)                                                                tttctatCAATGCCTCGCTTATCCGATAAAGTGCTTACGCAACCTTTGAGCGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGCTCCAGGAAAACTCTAATCGCTTCCCCCAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGCTTCGAAGCGATAGCGGGAGTAGGAGATCAGCAGCGGCATCACCTGTGgcc                                                                                                                                                                                      >  2:189577/5‑250 (MQ=255)                                                                           gCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGCGATAAGCAGTGGCATCACCTGTGGCAGAACGCCGt                                                                                                                                                                             >  2:164403/1‑249 (MQ=255)                                                                                      cAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTc                                                                                                                                                                >  1:14254/1‑251 (MQ=255)                                                                                          tAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGc                                                                                                                                                            >  2:294232/1‑251 (MQ=255)                                                                                                          ccGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGCTGATAAGCAACATCAGGCCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCAGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTgg                                                                                                                                           >  1:43973/1‑251 (MQ=255)                                                                                                                        aaTCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTcc                                                                                                                             >  1:7677/1‑251 (MQ=255)                                                                                                                               cagGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCAccgggc                                                                                                                      >  1:218215/1‑251 (MQ=255)                                                                                                                                gggCTGCCCGTGACGATGAGAAGCCCCATCAGGGCGAAGGTTTGTTGGAACTGGAACCCGCGAATCGCTCCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCAccgggcc                                                                                                                     <  2:109822/250‑1 (MQ=255)                                                                                                                                 ggCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCAccgggccg                                                                                                                    <  1:48111/251‑1 (MQ=255)                                                                                                                                           gACGATGATAAGCACCATAAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCg                                                                                                          >  1:78163/1‑251 (MQ=255)                                                                                                                                              gaggaaaaGCCCCATGAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTGGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGTCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCGTTGT‑GGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCtt                                                                                                       <  1:22343/245‑1 (MQ=255)                                                                                                                                                    aaGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCg                                                                                                 >  1:235139/1‑251 (MQ=255)                                                                                                                                                         ccATCGGGGCGCAGGTTTGTTGGACCTGGAACCCGCGAATCGCTTCCCCCAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCg                                                                                            <  2:239728/251‑1 (MQ=255)                                                                                                                                                         ccATCGGGGCGCAGGTTTGTGGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCg                                                                                            <  1:40059/251‑1 (MQ=255)                                                                                                                                                                    cAGGTTTGTTGGAACTGGCAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCtt                                                                                 >  2:186125/1‑251 (MQ=255)                                                                                                                                                                        tttGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCTGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCtgt                                                                                                                                                                                          <  1:42865/143‑1 (MQ=255)                                                                                                                                                                        tttGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCTGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCtgt                                                                                                                                                                                          >  2:42865/1‑143 (MQ=255)                                                                                                                                                                              ggAACTGGAACCCGCGAACCGCTCCCCACGGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGAGTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACg                                                                       <  2:255485/251‑1 (MQ=255)                                                                                                                                                                              ggAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGACCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACg                                                                       <  1:216344/251‑1 (MQ=255)                                                                                                                                                                                   tGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCggg                                                                  >  2:94111/1‑250 (MQ=255)                                                                                                                                                                                         ccGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGACAGCAGCTTGGAGAGCACGCCGGTGGtgtg                                                            >  2:113599/1‑251 (MQ=255)                                                                                                                                                                                             gAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGAt                                                         >  1:246291/1‑250 (MQ=255)                                                                                                                                                                                             gAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCGTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGGGTGGAt                                                         >  2:254631/1‑250 (MQ=255)                                                                                                                                                                                                    ttCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAgcg                                                 >  1:299651/1‑251 (MQ=255)                                                                                                                                                                                                      cccACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGCGGTGTGGATAAACAGcgcc                                               >  2:17194/1‑251 (MQ=255)                                                                                                                                                                                                               gggcGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGTTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGcgccagcacgcca                                      <  1:264925/248‑1 (MQ=255)                                                                                                                                                                                                                        tCCCGACTGCGCCGACCATGACGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGCGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCagcacgccagcacgcc                               >  2:140882/1‑249 (MQ=255)                                                                                                                                                                                                                          ccGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGCTAAACAGCGCCAGCACGCCAGCACGCCAGCg                           >  2:230159/1‑251 (MQ=255)                                                                                                                                                                                                                            gCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGa                          >  2:75064/1‑250 (MQ=255)                                                                                                                                                                                                                                        cATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGgtccgaggct             <  2:298419/251‑2 (MQ=255)                                                                                                                                                                                                                                            ccGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACg         >  1:249032/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cgGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCa  <  1:222538/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        CAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGC‑TTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCA  >  NC_000913/4322359‑4322851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑