Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,311,337	C→T	A236T (GCA→ACA)	alsB ←	D‑allose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,311,337	0	C	T	100.0%	109.4 / NA	35	A236T (GCA→ACA)	alsB	D‑allose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/12);  total (23/12)

CATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTCA  >  NC_000913/4311095‑4311581                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     catcatTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCt                                                                                                                                                                                                                                              >  2:241204/1‑251 (MQ=255)                           ggATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCa                                                                                                                                                                                                                    <  1:202078/251‑1 (MQ=255)                            gATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCAt                                                                                                                                                                                                                   >  2:249577/1‑251 (MQ=255)                                  aTCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCgcgc                                                                                                                                                                                                             >  1:177090/1‑251 (MQ=255)                                          gTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATc                                                                                                                                                                                                     <  1:82217/251‑1 (MQ=255)                                              aaaTTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCtt                                                                                                                                                                                                 <  1:300362/251‑1 (MQ=255)                                                aTTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGGTATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACATCCATTGCTATCGTGTCATTCGCCCAATAGATCGCttt                                                                                                                                                                                                >  1:103043/1‑250 (MQ=255)                                                  tCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCCACCATCCGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCCACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGCATGCCATCTGTACCGCCGACCAGCACTTTTCCCGTTTTTCCGTCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCtttcat                                                                                                                                                                                             >  2:181191/1‑248 (MQ=255)                                                                cAGCGAGATAATCTTGCCGGATTTCTCCGCGTCCACCATCAGCTTCAGACCCTGTGCGTCGATATCCGCCGGGTTCTGGGCAACCGACGCGGTCATTTGTCCGGCTGCCACCATTTTGCGTGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGTCGGTTGTGACTGCCTGAGCAACACCCATTGCCATCGTTTCATTCGCGCAATAGATCGCTTTAATCTTCGGAGTACGtt                                                                                                                                                                               <  2:262463/251‑1 (MQ=255)                                                                       aTAACCTTGCTGGAGTTCCCAGCGTCAACGATCACCTTCAGACCCGTTGCACCGATATCCGCCGGGTTCTGGGCAACCGTCGCGTCCATTTGTCCGGCGTCCACCATTTTGCGGGCTTCCGGAATGGCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACGCCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAacac                                                                                                                                                                        >  2:47355/1‑251 (MQ=255)                                                                        tAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACg                                                                                                                                                                       >  2:209562/1‑251 (MQ=255)                                                                           ccTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACAcc                                                                                                                                                                                                                                    <  2:108660/187‑1 (MQ=255)                                                                           ccTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACAcc                                                                                                                                                                                                                                    >  1:108660/1‑187 (MQ=255)                                                                                   gATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGAc                                                                                                                                                            <  2:168263/251‑1 (MQ=255)                                                                                                cAACCATCAGCTTCAGACCCGGTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATGTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTa                                                                                                                                               <  2:177090/251‑1 (MQ=255)                                                                                                        aacTTCAGACCCGTTGCTCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTGCGGCTTACACCATTTTGCGGGCTTCCGGAATGCCTTCTGTACCGACGGCCAGCACTTTTCCCGTTTTGCCGGCGTTTGTGACTGCCTGAGAAACACCAATTGCCAACGTGTCATTCGTGCAATAGATCGCTTTAATTTTCGGATTACGTTGCCTCCCGTTAGTGGCGCCATCCAGTGCTTTAATGCGGTc                                                                                                                                       >  2:168367/3‑251 (MQ=255)                                                                                                        aGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTc                                                                                                                                       >  2:10824/1‑251 (MQ=255)                                                                                                            tCAGACCCGTTGCGCCGATATCCGACGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGATTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTccc                                                                                                                                     >  1:109853/1‑249 (MQ=255)                                                                                                             cAGACCCGCTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCCCCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCACCACCCATTGCCATCGTGTCATTCGCGCAATAGCTCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGt                                                                                                                                  >  2:179145/1‑251 (MQ=255)                                                                                                                                     cgGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCt                                                                                                          >  2:59946/1‑251 (MQ=255)                                                                                                                                           cTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCt                                                                                                    >  2:51340/1‑251 (MQ=255)                                                                                                                                               gCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGgct                                                                                                >  1:139326/1‑251 (MQ=255)                                                                                                                                                     gTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCttt                                                                                          <  2:158014/251‑1 (MQ=255)                                                                                                                                                       cgcgGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATc                                                                                                                                                                                                                          >  2:8867/1‑121 (MQ=255)                                                                                                                                                       cgcgGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATc                                                                                                                                                                                                                          <  1:8867/121‑1 (MQ=255)                                                                                                                                                                      ggCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTgg                                                                         <  1:249577/251‑1 (MQ=255)                                                                                                                                                                            caccaTTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCAt                                                                   >  2:247053/1‑251 (MQ=255)                                                                                                                                                                            caccaTTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTGGCTTTTTTGAAGGCTTCGGTGGCACCAt                                                                   <  1:261093/251‑1 (MQ=255)                                                                                                                                                                                          cTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTc                                                      >  2:34545/1‑250 (MQ=255)                                                                                                                                                                                                     ccATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGtt                                          >  1:120427/1‑251 (MQ=255)                                                                                                                                                                                                          tGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCgg                                     >  1:138999/1‑251 (MQ=255)                                                                                                                                                                                                               cgacgaCCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCttt                                 >  1:143108/1‑250 (MQ=255)                                                                                                                                                                                                                   gatcAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGATTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACATGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGAGTTAACGGCTTTACCCt                            >  1:39530/4‑251 (MQ=255)                                                                                                                                                                                                                   gacCAGCACTTTTCCCGTTTTTCCGGCGTTTGTGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCt                            >  1:39533/1‑251 (MQ=255)                                                                                                                                                                                                                                             cGTTTGTGACTGACTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCGTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGTCGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTCa  <  1:291219/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCATTTTGCGGGCTTCCGGAATGCCATCTGTACCGACGACCAGCACTTTTCCCGTTTTTCCGGCGTTTGCGACTGCCTGAGCAACACCCATTGCCATCGTGTCATTCGCGCAATAGATCGCTTTAATATTCGGATTACGTTGCAACACGTTAGTGGCGACATCCAGTGCTTTAATGCGGTCCCAGTCGGCAGGCTGGCTGGCGACAAGCTTGATCTGGCTTGCTTTTTTGAAGGCTTCGGTGGCACCATTACGACGCGCTTCACCGGAGGCGTTACCGGCTTTACCCTCAATGATTGCGACTTCACCACCTTCA  >  NC_000913/4311095‑4311581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑