Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,301,071	C→T	A670T (GCA→ACA)	mdtO ←	putative multidrug efflux pump subunit MdtO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,301,071	0	C	T	93.3%	34.0 / NA	15	A670T (GCA→ACA)	mdtO	putative multidrug efflux pump subunit MdtO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (3/11);  minor base G (1/0);  total (4/11)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATCCCAGG  >  NC_000913/4300827‑4301310                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                cTGCGGGTCATTGCGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCggggg                                                                                                                                                                                                                                           >  2:15592/1‑250 (MQ=255)     gggTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTc                                                                                                                                                                                                                                       <  2:194709/251‑1 (MQ=255)       gTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGtgcc                                                                                                                                                                                                                                     >  2:78376/1‑248 (MQ=255)                   tttCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTGTATGGCGGGGCGGTTCAGTCTGGCAAg                                                                                                                                                                                                                           >  2:291719/1‑249 (MQ=255)                                                       aaGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCGGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTtaa                                                                                                                                                                                     <  2:82062/251‑1 (MQ=255)                                                                atCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTTGCCACGGGGCGGAAAGCTGGCAGACGGGTTGCTCCCGtttttattagggggg                                                                                                                                                                            >  2:301750/1‑240 (MQ=255)                                                                atCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCGGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTgg                                                                                                                                                                             <  2:255600/250‑1 (MQ=255)                                                                     cgGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGGTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAAGAAGGTGGGCAgg                                                                                                                                                                        <  2:302771/250‑1 (MQ=255)                                                                          aGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGcc                                                                                                                                                                  <  2:186707/251‑1 (MQ=255)                                                                                       ccGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCTTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATTTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgt                                                                                                                                                      <  1:253076/250‑1 (MQ=255)                                                                                                                                                       cgtGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTtctc                                                                                     <  2:105245/251‑1 (MQ=255)                                                                                                                                                               cGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGt                                                                             <  2:244340/251‑1 (MQ=255)                                                                                                                                                                                      cGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTcc                                                      <  1:87330/251‑1 (MQ=255)                                                                                                                                                                                                                      ccTTGTGCCTGTTCCGTGGCGGGCGTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGcc                        <  1:116716/249‑1 (MQ=255)                                                                                                                                                                                                                                          gggcgTTAATGTCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTTACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCTAGTTCCAGGTGGCATCCCAgg  <  2:137059/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                CTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGGAGCTGGCAAGATGAATATCGTCGGCCAGTTTGATTTGTTCCGGTTTGAGCTGTTGATGAGGTGCAGAATCCTTACGTACCAGGGCACAGCCGGAAATCAGCGTCGTGCTGCCGAGAATGCTGCACAACAGCAGACGTGAAAGTTGACGATTGATCATTGCGTGGCTCCTTGTGCCTGTTCCGTGGCGGGCGTTAATGCCGGGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAACTGACCTGCTCTGTCCGGCTGTAGTGCGTTATCCAGCGCCTGCGCCGAGTTCCAGGTGGCATCCCAGG  >  NC_000913/4300827‑4301310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑