Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,033	C→T	A447T (GCG→ACG)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,033	0	C	T	95.7%	137.4 / NA	46	A447T (GCG→ACG)	acs	acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/21);  minor base G (0/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCC  >  NC_000913/4285788‑4286274                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ccccAGACGGTGACCGGAGACGTTCAGCACTTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGt                                                                                                                                                                                                                                               <  1:1119/250‑1 (MQ=255)     aGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCAc                                                                                                                                                                                                                                          >  2:95026/1‑251 (MQ=255)               cGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAAc                                                                                                                                                                                                                                <  1:286200/251‑1 (MQ=255)                  agaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGa                                                                                                                                                                                                                             <  1:56482/251‑1 (MQ=255)                         cAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCAACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTg                                                                                                                                                                                                                      >  2:137846/1‑251 (MQ=255)                                          gCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGc                                                                                                                                                                                                     >  2:256729/1‑251 (MQ=255)                                                  aTCCAGTAATAGCCATCTTCAGCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTTCCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGTTAgc                                                                                                                                                                                             <  2:65878/251‑1 (MQ=255)                                                     cAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGcc                                                                                                                                                                                          >  2:260229/1‑251 (MQ=255)                                                            aGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAg                                                                                                                                                                                    <  2:22689/250‑1 (MQ=255)                                                               cATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCggg                                                                                                                                                                                <  1:260229/251‑1 (MQ=255)                                                                      aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATc                                                                                                                                                                          >  2:279967/1‑250 (MQ=255)                                                                      aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCa                                                                                                                                                                         <  2:272224/251‑1 (MQ=255)                                                                               cgcgcCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAAccgc                                                                                                                                                                >  2:41079/1‑251 (MQ=255)                                                                                    cGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGtt                                                                                                                                                           <  2:137497/251‑1 (MQ=255)                                                                                      tCGCCGCTGAAATACATATATTTGAAGGGGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTCCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGCTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTc                                                                                                                                                         >  2:48132/1‑251 (MQ=255)                                                                                         ccgcTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGt                                                                                                                                                      >  1:231450/1‑251 (MQ=255)                                                                                                    cATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAgg                                                                                                                                           >  1:141796/1‑251 (MQ=255)                                                                                                       atTTTGGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTa                                                                                                                                         <  1:11841/250‑1 (MQ=255)                                                                                                            tGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGacc                                                                                                                                   >  1:101236/1‑251 (MQ=255)                                                                                                               aGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGaccacc                                                                                                                                >  2:70763/1‑251 (MQ=255)                                                                                                               aGGTGGAGAAGTAGGTCTGTTAAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCGGACCCGGCCAGGAGTCGGTGATTCCCAGGCTACCTTCGGTGG‑CCCCCCCCAGCGGGTTACCTTCTTTATCGACCAGCGTCGGTTGCACGCCAAAGAACGGACGGGTTGCCGAACCGGCTTGCAGCTCGGTAGCGCCAGGCAGAGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGaccacc                                                                                                                                <  1:144452/251‑1 (MQ=255)                                                                                                                 gTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCgg                                                                                                                              >  2:88611/1‑251 (MQ=255)                                                                                                                  tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCg                                                                                                                                                                                                                   <  1:31220/165‑1 (MQ=255)                                                                                                                  tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCg                                                                                                                                                                                                                   >  2:31220/1‑165 (MQ=255)                                                                                                                  tGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCgg                                                                                                                              >  2:159028/1‑250 (MQ=255)                                                                                                                   ggAGAAGTAGGTCTGTTCAAAACGTGCGGGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCGACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGGCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTTAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGAc                                                                                                                            <  2:148359/251‑1 (MQ=255)                                                                                                                      gaAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCAACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACAtt                                                                                                                         <  1:137846/251‑1 (MQ=255)                                                                                                                                       aaCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAttttt                                                                                                        >  2:118484/1‑251 (MQ=255)                                                                                                                                                   cGCCAAACAGAGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGCTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCAc                                                                                            >  2:117910/1‑251 (MQ=255)                                                                                                                                                    gCCAAACAGCGTAAGCGCCTGACCGGGCCAGGAGTCGGGGATTACCAGGCTCCCTGCGGTGG‑CCCCCTCCAGCGGGTTCCCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACt                                                                                           <  2:231450/251‑1 (MQ=255)                                                                                                                                                    cccAAACAGCGTCCGCGCCTGCCCCGACCAGGAGTCGGTGATTACCAGGTTACCTTCG‑TGGACCCCCTCCAGCGGGTTCCCTTCGTTATCGACAGGCGGCGGTGCCACGCTGAAGAACGGCCTTGTTGCCGACCCGGTTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGTCCAGGAATCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACt                                                                                           <  2:169773/250‑1 (MQ=255)                                                                                                                                                       aaaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTccc                                                                                        >  1:47849/1‑251 (MQ=255)                                                                                                                                                            gCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCATTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCt                                                                                   >  2:149679/1‑251 (MQ=255)                                                                                                                                                                   cgcCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGGGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGt                                                                            <  1:232789/251‑1 (MQ=255)                                                                                                                                                                           ccGGCCAGGATTCGGTGATTACCAGGCTCCCTTCGGTGG‑CCCCCTCCAGCGGTTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGc                                                                    <  1:69952/251‑1 (MQ=255)                                                                                                                                                                           ccGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGc                                                                    >  1:255451/1‑251 (MQ=255)                                                                                                                                                                                   gagtgggggATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTGCCTTCGTTATCGACCGGCGTCGGTGGCACGCCGAAGAACGGACGGGTGGCCGAACCGGCTTTCAGCGCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTGCCGGGTTAATTGGCTCGCCCAc                                                            <  2:61562/243‑1 (MQ=255)                                                                                                                                                                                             ttACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCCTCGTTATCGACCAGCGTAGGTTGCACGCGGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTGTCGGTCTGCCACCGGGTATCGACCACCGGACATTTATCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGGTAATTGGCTCGCCCACGGAACCg                                                     >  1:14341/1‑248 (MQ=255)                                                                                                                                                                                              tACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGCCCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTg                                                                      <  1:136454/230‑1 (MQ=255)                                                                                                                                                                                              tACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGCCATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTg                                                                      >  2:136454/1‑230 (MQ=255)                                                                                                                                                                                                      tACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGCATGCGCAGc                                         >  2:190449/1‑251 (MQ=255)                                                                                                                                                                                                            cGGTGG‑CCCCCTCCAGCGGGTTCCCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGaa                                   <  1:168312/251‑1 (MQ=255)                                                                                                                                                                                                                gg‑CCCCCTCCAGCGGTTTACTTGCGTTATCGACCGGGGTGGGTTCCACGCCGAAGAACGGAAGGGTGGCCGAACCTGCTTTCAGCTCGGGAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACGCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCTACGAAcggt                               <  2:180010/251‑1 (MQ=255)                                                                                                                                                                                                                          cAGCGGGTTACTTTCTTTATCGCCCAGCGTCGGTTGCACGCCGAAGAACGGACGGGTTGCCGAACGGGCTTTCGGCTCGGTAGCGCCAGGCAGCGGGGGGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCtt                      <  1:41079/251‑1 (MQ=255)                                                                                                                                                                                                                               ggTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTGGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATc                 <  2:253477/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGACCAGCGTCGGTTGCCCGCCGGAGAACGGACGTGTTGCCGAAGCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGGTTCGGACTGCCACCAGGTATCGACCACCGGACATTTCTTGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTACGGGCTAATTGGCTCGCCCACGGAACTGAGAATGCGCAGCGACGCAGGGTCGGGGCCTGCGATCGCTTTATCGCCTTcc  >  2:228430/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGG‑CCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCC  >  NC_000913/4285788‑4286274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑