Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,077	C→T	A276T (GCG→ACG)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,077	0	C	T	100.0%	97.3 / NA	28	A276T (GCG→ACG)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/10);  total (18/10)

CCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACA‑GCAACAGCACGGCTTTGATAATTTGC  >  NC_000913/4283830‑4284282                                                                                                                                                                                                                                                        |                                                                                                                                                                                                               ccGAGGAACAGGCTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGCTTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCcgtcgt                                                                                                                                                                                                             >  2:114137/1‑251 (MQ=255)                      ccTGCATTCGCCAGGTGAACGGCCGCCAGGTTGTTACCACCAATCAGATGGCCCGCCGCGGCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTcccagacc                                                                                                                                                                                        <  1:200593/250‑1 (MQ=255)                                     tGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCgg                                                                                                                                                                          >  2:168774/1‑250 (MQ=255)                                                  tgttgtTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTc                                                                                                                                                            >  2:141662/1‑251 (MQ=255)                                                        tACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAgc                                                                                                                                                      >  1:294304/1‑251 (MQ=255)                                                                          ccgccgCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCatgatg                                                                                                                                    >  1:253328/1‑251 (MQ=255)                                                                           cgccgcGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGt                                                                                                                                   <  2:177919/251‑1 (MQ=255)                                                                              cgcgTCTTTATATTCCGGATTCGCAGCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGCATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGACAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGACCGGATCTTTCACCAGCCCGCCCGGCTTCATGATTTCg                                                                                                                                 >  2:120429/1‑250 (MQ=255)                                                                                   cTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCt                                                                                                                           >  1:242767/1‑251 (MQ=255)                                                                                                   cGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCa                                                                                                                                                                                                         <  2:158479/157‑1 (MQ=255)                                                                                                   cGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCa                                                                                                                                                                                                         >  1:158479/1‑157 (MQ=255)                                                                                                    gCACCAACCAGCATGATCGCGCGGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCAt                                                                                                          <  2:98602/251‑1 (MQ=255)                                                                                                             aGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTGCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACt                                                                                                 <  1:21608/251‑1 (MQ=255)                                                                                                                     cgcgCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTATAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGAt                                                                                         <  2:262915/251‑1 (MQ=255)                                                                                                                              gCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTGCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGc                                                                                <  2:202428/251‑1 (MQ=255)                                                                                                                               ccGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCTCTTCACTGAACAGATTGTTGAAGCt                                                                               >  1:29776/1‑251 (MQ=255)                                                                                                                                          gatgaGAATATAGAAGTAGCACATAAACCCGGTCGCATAGAACACGCTCTTACGAGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCCAACATCAGTCCCAGACCCATAGAGCGCGCGGAGATCGGACCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGCAGCTaaacacgacgc                                                                    >  2:293044/5‑243 (MQ=255)                                                                                                                                               gAATATAGAAGTAGCCCATAAACCCGGAGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtc                                                               >  1:225111/1‑251 (MQ=255)                                                                                                                                                 atatAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtcat                                                             <  1:228554/251‑1 (MQ=255)                                                                                                                                                     aGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCAcc                                                         >  1:277895/1‑251 (MQ=255)                                                                                                                                                      gAAGTCGCACATACACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCCGCCCGTAAAGAAGCGCATCAGCATGTGCGGCAAGCCCGTCGCACCAACCATCAGTCCCAGACCCAGAGAGAGCGCGGAGCTCGGCTCTTTCACCAGCCCGCCCGTCTTCATCATGTCGACACCTTTCGGGTGTACAGCCATCGCTTCACTGCACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCAcc                                                         >  2:14286/1‑250 (MQ=255)                                                                                                                                                           aGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAg                                                   <  2:218293/251‑1 (MQ=255)                                                                                                                                                           aGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGAGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATaa                                                     >  1:16406/1‑249 (MQ=255)                                                                                                                                                                  aaaCCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATa                                             >  1:19872/1‑250 (MQ=255)                                                                                                                                                                           ggCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCAc                                   >  2:140370/1‑251 (MQ=255)                                                                                                                                                                               tAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGa                                >  1:280489/1‑250 (MQ=255)                                                                                                                                                                                            tACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGaaca‑gcaacagctc                  <  2:100840/250‑3 (MQ=255)                                                                                                                                                                                                       cgGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACA‑GCAACAGCACGGCTTTGATAAt      >  1:84303/1‑251 (MQ=255)                                                                                                                                                                                                           cATCGCTGGCTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATAGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGCCGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGA‑CACGCAACAGCACGGCTTTGATAATTTGc  >  2:191041/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                               CCGAGGAACAGGTTGCCGCCCACTGCATTCGCCAGGTGAACGGCCGCCATGTTGTTACCACCAATCAGATGGCCCGCCGCGTCTTTATATTCCGGATTCGCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCATCACCATAAAGGCCATAAAGCTGGCACCGAACA‑GCAACAGCACGGCTTTGATAATTTGC  >  NC_000913/4283830‑4284282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑