Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,536	C→T	Q180Q (CAG→CAA)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,536	0	C	T	100.0%	53.9 / NA	17	Q180Q (CAG→CAA)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/5);  total (12/5)

GATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATTATTTGC  >  NC_000913/4282301‑4282781                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      gATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGttt                                                                                                                                                                                                                                        >  1:235214/1‑251 (MQ=255)      aCCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGt                                                                                                                                                                                                                                     >  1:3980/1‑249 (MQ=255)                                                                                   aTTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGACATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAAt                                                                                                                                                      >  1:171308/1‑251 (MQ=255)                                                                                      gTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGt                                                                                                                                                   <  1:40215/251‑1 (MQ=255)                                                                                             tGAACATGCATAGCGGAACATGAATTTAGAGTACCCTCAGTCCTTTTTATCTGAGCGAAAATTGAGTCATCGAGAATACAATTATTAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCCAAATTCGAATTGGATAAATCTTCATCTTTAATCACGAAACCTTTCAGGCTAAGGTGTGAAAAATCGTAaccacc                                                                                                                                            >  2:268642/1‑251 (MQ=255)                                                                                               aaCATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCAt                                                                                                                                          >  2:232067/1‑251 (MQ=255)                                                                                                   tGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTAc                                                                                                                                      >  1:139375/1‑251 (MQ=255)                                                                                                                 tGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGAttt                                                                                                                        >  1:121037/1‑251 (MQ=255)                                                                                                                           gTTCCGTCAGTCATTTTTATCGGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGCTTTCGATAGtatt                                                                                                              >  1:8520/1‑251 (MQ=255)                                                                                                                                                tGAGCGAAATTTGAGTCATCGCGAATACAATTTTCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGTTGACattatt                                                                                         >  2:39882/1‑251 (MQ=255)                                                                                                                                                                cATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATaccacc                                                                         >  1:244939/1‑251 (MQ=255)                                                                                                                                                                                            tttttCCATTATGGCGAAATTCAAATTCGATTTCTACATCTTACAGTCTTGAACAATCGCGTTTTGTCGTCTGCAACCTGCAAAATTCGAATTGGATAACTCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACAGTTCCTTATCTttatta                                              >  2:23515/1‑250 (MQ=255)                                                                                                                                                                                              tttCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAg                                            <  2:121037/250‑1 (MQ=255)                                                                                                                                                                                                      aTGGCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAActc                                    <  2:269481/250‑1 (MQ=255)                                                                                                                                                                                                         gCGAAATTAAAATTCGTTTTATACATATTACAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACtcttct                                <  1:232067/251‑1 (MQ=255)                                                                                                                                                                                                                                      aCAGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATTAttt    <  2:280097/250‑1 (MQ=255)                                                                                                                                                                                                                                        aGTCTTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATTATTTGc  >  1:162130/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      GATTTACCTCAGCCATGTATGCCATATAAAAATTTGAATAATCAAGGAAGGTATTTTTAATATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTTCAGGCTAAGGTGTGAAAAATCGTAACCACCATTTACTGTCTGTGGGATTTCGATAGTATTATTATGGACGGTGACATTATTTATAAAATATACCACCTTAATTGACACTTCCTTATCTTTATTAAGGTAAACTCTTCTCGTAATTCTATCGTCACATTTATTATTTGC  >  NC_000913/4282301‑4282781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑