Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,278,488	C→T	P4S (CCA→TCA)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,278,488	0	C	T	100.0%	38.5 / NA	13	P4S (CCA→TCA)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

CATCGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAA‑CGTGGAAGCACTGTCCGTCAGGAAGTGGTT‑GCCGGGTTAACAACGTTTCTGGCGATGGTCTACTC‑GGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGG‑CTTCCCGCCTGCGGCAGTTTTCGTTG‑CAACCTGTCTGGTTGCCGGACTCGGTTCTA  >  NC_000913/4278241‑4278695                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                      cATCGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCCGGGGATGTTTTCTTATGTCTACGTCAt                                                                                                                                                                                                                    >  1:221798/1‑251 (MQ=255)        aaGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCcgcgcg                                                                                                                                                                                                             >  2:236975/1‑251 (MQ=255)         aGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGCTGTTTTCTTATGTCTACGTCATCAgcgagt                                                                                                                                                                                                            >  1:15642/1‑248 (MQ=255)                                             gAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAAGTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCGGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTGATGGCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTc                                                                                                                                                                        <  1:298982/250‑1 (MQ=255)                                                                ccGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAA‑CGTGGAAGCACTGTcc                                                                                                                                                   <  1:297214/251‑1 (MQ=255)                                                                            ttGCTTTTTTTACCACACGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGGCCGCTGGTTT‑TAAATTTCACCA‑CGTGGAAGCACTGTCCGTCAGGAAGTgg                                                                                                                                       >  2:155211/1‑251 (MQ=255)                                                                               cTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCGACGCCCGGGTT‑AAAATTTCACAA‑CGTGGAAGCACTGTCCGTCAGGaaagggttg                                                                                                                                    >  1:233524/1‑244 (MQ=255)                                                                                 ttttttACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAA‑CGTGGAAGCACTGTCCGTCAGGAAGTGGTT‑g                                                                                                                                   <  2:112674/249‑1 (MQ=255)                                                                                                                                 gCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAA‑CGTGGAAGCACTGTCCGTCAGGAAGTGGTT‑GCCGGGTTAACAACGTTTCTGGCGATGGTCTACTC‑GGTCATCGTCGTTCCa                                                                                <  1:155211/251‑1 (MQ=255)                                                                                                                                     cGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGGTCACTCCACGCCTGGTTT‑AAAACTTTACCA‑CGTGGGAGCACTGTCCGCCAGGGAGTGGTT‑TCCGGGTTAAAAACGTTTCATGCGATGGTCTACTC‑GGTCAACGTCGTTCCCGGt                                                                             >  1:216449/1‑250 (MQ=255)                                                                                                                                                            gAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAA‑CGTGGAAGCACTGTCCGTCAGGAAGTGGTT‑GCCGGGTTAACAACGTTTCTGGCGATGGTCTACTC‑GGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGG‑CTTCCCg                                                    <  2:24515/251‑1 (MQ=255)                                                                                                                                                                                             tGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTCACTCCACCCCCGGTTTTAAAATTT‑ACAAACG‑GGGAGCACCGGCCCTCAGGGAGTGGTTTCCCGGGTTA‑CAACGTTTCTCGCGGTGGGCTACTCCGGT‑ATCGTCGTTCTCGGGATGTTTGGTAAAACAGGGCTTCCCCCCCGCGGC‑GTTTTTTTTGGCAACCTG‑CTGGtt                  >  2:301594/1‑251 (MQ=255)                                                                                                                                                                                                             tGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGTCATCAGCGCGTACCGGCGGTTTACTCGACGCCTGGTTT‑AAAATTTTACAA‑CGTGGAAGGCCTGTCCGGCAGGAAGGGGTT‑GCCGGGGTAACAACGTTTCTGGCGATGGTCTTCTC‑GGTCATCGTCGTTCCCGGTATGTTTGGTAAAGCAGG‑CTTTCCCCCTGCGGCCGGTTTTGTTT‑CAACCTggcttggttcccgactccggtctt  >  2:5207/1‑228 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                      CATCGTCAAGACAATAAAAGAGAAAAAAGCAGCAAACTTCGGTTGAAAAAGCCGCTATGATCGCCGGATAATCGTTTGCTTTTTTTACCACCCGTTTTGTATGCGCGGAGCTAAACGTTTGCTTTTTTGCGACGCAGCAAATTGTCGCAAACCTGGAGCAGGAAGATAACGTTTCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTT‑AAAATTTCACAA‑CGTGGAAGCACTGTCCGTCAGGAAGTGGTT‑GCCGGGTTAACAACGTTTCTGGCGATGGTCTACTC‑GGTCATCGTCGTTCCAGGTATGTTGGGTAAAGCAGG‑CTTCCCGCCTGCGGCAGTTTTCGTTG‑CAACCTGTCTGGTTGCCGGACTCGGTTCTA  >  NC_000913/4278241‑4278695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑