Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,269,449	C→T	C12C (TGC→TGT)	aphA →	acid phosphatase/phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,269,449	0	C	T	100.0%	43.3 / NA	15	C12C (TGC→TGT)	aphA	acid phosphatase/phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/4);  total (11/4)

ATCCTTACATCGAGTAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCT  >  NC_000913/4269212‑4269678                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      aTCCTTACATCGAGTAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGctc                                                                                                                                                                                                                          >  1:28447/1‑251 (MQ=255)         aTCGAGCAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAACAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGt                                                                                                                                                                                                                  >  2:285128/1‑251 (MQ=255)                                       aaGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAGTATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCa                                                                                                                                                                                    <  1:49849/251‑1 (MQ=255)                                                            ggTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAAc                                                                                                                                                               <  1:29604/251‑1 (MQ=255)                                                                      aaTCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGCCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAATAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGc                                                                                                                                                     >  1:24400/1‑251 (MQ=255)                                                                                                    aGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGttt                                                                                                                       >  1:186387/1‑251 (MQ=255)                                                                                                          tccttcctTTCCCCGCCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGCCTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCg                                                                                                                 >  1:76760/1‑251 (MQ=255)                                                                                                                    ccccGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTTTTGCCCTGGCCTCAGCTCCTTCACCGCGTATTCCTGGGACTATCGTGGCCAGGCTGGCTGAACAGGCAGCCATTCATTGGGTTTCGGTCGCACAAATTg                                                                                                        <  1:27232/250‑1 (MQ=255)                                                                                                                             cTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCt                                                                                              >  2:87789/1‑251 (MQ=255)                                                                                                                                        gTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTc                                                                                   >  1:159033/1‑251 (MQ=255)                                                                                                                                                                                             aTTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCACTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCACTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCCGGGCGTCCGCCAATGGCGGTGGGGGTTGATATCTCTGACACGGAACTTTTTTCCAGTCCg                              >  1:44111/1‑251 (MQ=255)                                                                                                                                                                                                     aaaaaTATGCGCAAGATCACACAGGCAACCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGCGGGGGTTGATATCGGTGACAACGTACTTTTTTCCCGTCCGGGCctctt                      >  2:35767/1‑250 (MQ=255)                                                                                                                                                                                                       aaaTATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGGACTTTTTTCCAGTCCGGGCTTCTggg                    >  1:9674/1‑250 (MQ=255)                                                                                                                                                                                                                    aTCACACAGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAAc       <  1:90229/251‑1 (MQ=255)                                                                                                                                                                                                                         acaGGCAATCAGTGCCGTTTGTTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGCGGGGGTTGATATAGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGGGAGGCAAAAAAAACTtct  >  2:102383/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      ATCCTTACATCGAGTAATAAACATTTTTTATACAAAAAAAGACAGGAACGTATTTACTGGGTTAAATATAATCATCCTGCTTTTCATCACAAAAACCGCAGATAATCCTTCCTTTCCCCGGCAGCTGGCGTTATGGTCAGATGGTTTTTGCAACAAATCTCACAATAAAAAGTTTCAACATACTGACTATTTAGGGAAAAATATGCGCAAGATCACACAGGCAATCAGTGCCGTTTGCTTATTGTTCGCTCTAAACAGTTCCGCTGTTGCCCTGGCCTCATCTCCTTCACCGCTTAACCCTGGGACTAACGTTGCCAGGCTTGCTGAACAGGCACCCATTCATTGGGTTTCGGTCGCACAAATTGAAAATAGCCTCGCAGGGCGTCCGCCAATGGCGGTGGGGTTTGATATCGATGACACGGTACTTTTTTCCAGTCCGGGCTTCTGGCGCGGCAAAAAAACCTTCT  >  NC_000913/4269212‑4269678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑