Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,037,292	G→T	D182Y (GAC→TAC)	hyaF →	protein HyaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,037,292	0	G	T	100.0%	108.2 / NA	32	D182Y (GAC→TAC)	hyaF	protein HyaF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/23);  total (9/23)

CAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGG  >  NC_000913/1037049‑1037520                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     cagGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCa                                                                                                                                                                                                                               <  1:39793/251‑1 (MQ=255)           acAGTTAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTAGGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGTAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGc                                                                                                                                                                                                                     <  2:271416/251‑1 (MQ=255)                                gggCGATCTTCGGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCGGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTActc                                                                                                                                                                                                <  2:158813/250‑3 (MQ=255)                                         tctGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGc                                                                                                                                                                                          <  2:206361/248‑1 (MQ=255)                                         tctGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCa                                                                                                                                                                                            <  2:83198/246‑1 (MQ=255)                                            gCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGc                                                                                                                                                                                     <  1:193207/250‑1 (MQ=255)                                              ggATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGa                                                                                                                                                                                  <  1:194067/251‑1 (MQ=255)                                                   aTGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCgg                                                                                                                                                                              <  2:78790/250‑1 (MQ=255)                                                      gCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGTGGCTGATCGGCttt                                                                                                                                                                          <  1:249875/251‑1 (MQ=255)                                                                    gTCGCGGCGAACAGATGCTGGAGGACAAACTGCAGGCTGGCTGCGCGCCGCTGGCGTTGCGGCAGGGGGCAACGCAAAATATACTGCCGACAGATTCGCTGTTAACGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTTGCACATGTACGTAACCCCTACGCGCAGCCGCACCGCATTAATCTGACGCAATTACCCATTAGCGAGGCTGATCGGCTTTTTTTCTCCCGtctt                                                                                                                                                            >  2:28904/1‑250 (MQ=255)                                                                             aaaaGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCAGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg                                                                                                                                                   <  2:10059/251‑1 (MQ=255)                                                                                            aCAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTGGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGAtt                                                                                                                                    <  2:193663/251‑1 (MQ=255)                                                                                              aaacgggctgcgggctgCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCg                                                                                                                                  <  2:13268/239‑1 (MQ=255)                                                                                                aCTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGt                                                                                                                                 >  2:94091/1‑250 (MQ=255)                                                                                                      ggctggctGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAg                                                                                                                                                                                       <  2:111353/190‑1 (MQ=255)                                                                                                      ggctggctGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAg                                                                                                                                                                                       >  1:111353/1‑190 (MQ=255)                                                                                                        ctggctgCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc                                                                                                                        >  2:278280/1‑251 (MQ=255)                                                                                                                        ggCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGGCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACGCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCAGCAGGGAGGCTGGTCGGCTTTTTCGCTGACGTGGCGGTGGGCCGGGAAATATGGAGTTTTGTAGCATTGGCTCTGGCGAGAGCta                                                                                                          <  2:303091/249‑1 (MQ=255)                                                                                                                                ggCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCAtt                                                                                                                           <  1:5055/224‑1 (MQ=255)                                                                                                                                ggCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCAtt                                                                                                                           >  2:5055/1‑224 (MQ=255)                                                                                                                                          aaCGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTAc                                                                                      >  2:12207/1‑251 (MQ=255)                                                                                                                                          aaCGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTAc                                                                                      <  2:304330/251‑1 (MQ=255)                                                                                                                                                        tGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGc                                                                          <  1:91050/249‑1 (MQ=255)                                                                                                                                                                    cGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGATAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTAc                                                             <  1:39108/250‑1 (MQ=255)                                                                                                                                                                           aCCGCCGCCCATTGATGGCCTGATGACTGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCt                                                     >  2:36193/1‑251 (MQ=255)                                                                                                                                                                                     aTTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGccc                                           <  1:94091/251‑1 (MQ=255)                                                                                                                                                                                         aTGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGtt                                        >  1:71881/1‑250 (MQ=255)                                                                                                                                                                                           ggCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACt                                     <  1:281434/251‑1 (MQ=255)                                                                                                                                                                                                           tACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATc                     >  1:74224/1‑251 (MQ=255)                                                                                                                                                                                                                    cgcATGAGTTACTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATa            <  1:261561/251‑1 (MQ=255)                                                                                                                                                                                                                         gAGTTACTGGCACATGTACTTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCgg        <  1:49111/250‑1 (MQ=255)                                                                                                                                                                                                                              aCTGGCACATGTACGTAACCCCTACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAgtgg  <  2:208339/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                     CAGGCTGACGACAGTGAAAGTGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGG  >  NC_000913/1037049‑1037520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑