Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,249,684	C→T	A44V (GCT→GTT)	malM →	maltose regulon periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,249,684	0	C	T	100.0%	108.1 / NA	32	A44V (GCT→GTT)	malM	maltose regulon periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/11);  total (21/11)

AAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTCC  >  NC_000913/4249449‑4249926                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   aaaaCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTACATCTGTTGCGCTGCaacaacc                                                                                                                                                                                                                                     >  2:50714/1‑250 (MQ=255)  aaaCAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTc                                                                                                                                                                                                                                    >  1:203867/1‑251 (MQ=255)        gTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTgg                                                                                                                                                                                                                              <  1:92055/251‑1 (MQ=255)           gTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGATACCGCTGAGCTCCAGATTGTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTGGCCGATGTTAACTTCGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCGTCTGTTGCGCTGCAACAACTCACCTGGac                                                                                                                                                                                                                            <  2:295479/250‑1 (MQ=255)                     gAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTGTATCAGCAGGGGTACTGGCAAGCGCGCCTGGAATGAGCCTTGCCGAGGTTAACTGCGTACCGTAAAACACCAGCTACTCGCCAGCCGGTCCATCGGTTGCGCGGCATCAATTCACCTGGACTCCGGTc                                                                                                                                                                                                                     <  2:142085/247‑1 (MQ=255)                            gCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTaa                                                                                                                                                                                                          <  1:134115/251‑1 (MQ=255)                                           aTTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCa                                                                                                                                                                                           <  1:47641/251‑1 (MQ=255)                                                   gTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCg                                                                                                                                                                                    >  1:166121/1‑250 (MQ=255)                                                                   ccTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACt                                                                                                                                                                   >  2:305422/1‑251 (MQ=255)                                                                            cGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTcc                                                                                                                                                          >  1:144546/1‑251 (MQ=255)                                                                                     ccAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATc                                                                                                                                                   <  2:180927/249‑1 (MQ=255)                                                                                       aGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTg                                                                                                                                               >  2:240046/1‑251 (MQ=255)                                                                                              gAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCgg                                                                                                                                         <  2:38141/250‑1 (MQ=255)                                                                                                                atgaaTAAAAGTCTCATCGTCATCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACAAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTACAACCCAGACCACCCAACTGGCGACCGGAGGCCAACAATTGAACGATCCCGGCATCAGTGGTCACGTTGCTGCGTCAAGCGTccc                                                                                                                      >  2:296280/1‑251 (MQ=255)                                                                                                                           tctcATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTg                                                                                                           >  1:195045/1‑251 (MQ=255)                                                                                                                              cATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCg                                                                                                        >  2:119505/1‑251 (MQ=255)                                                                                                                               aTCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGACTTGCCGCTGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCg                                                                                                        >  1:276878/1‑250 (MQ=255)                                                                                                                                       ctGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGAccc                                                                                               >  1:147199/1‑251 (MQ=255)                                                                                                                                                             gCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAATCACCAGCAGCGCGCCTGCCATTCCAGCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGCCGCTGACCAGCGACGTGaa                                                                         <  2:283311/251‑1 (MQ=255)                                                                                                                                                                    cgcCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTACATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACTACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGaacaaaca                                                                   >  2:212857/1‑250 (MQ=255)                                                                                                                                                                       cTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAAcc                                                               >  2:276943/1‑251 (MQ=255)                                                                                                                                                                         ggAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAAcc                                                               <  1:302715/249‑1 (MQ=255)                                                                                                                                                                                gCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGttttt                                                      >  2:62401/1‑251 (MQ=255)                                                                                                                                                                                    tGCCGATGTTAACTACGTACCGCAAAACACCCGCGACGAGCCCGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCACCTGGCGACCGGCGGCCACCAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGcc                                                                                                                  >  1:184805/1‑186 (MQ=255)                                                                                                                                                                                    tGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCATTGGTCCGGTTGCTGCGTACAGCGTCCCGGCa                                                                                                                  <  2:184805/187‑1 (MQ=255)                                                                                                                                                                                            ttAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTg                                          >  1:104406/1‑251 (MQ=255)                                                                                                                                                                                                    gTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCt                                  >  2:144358/1‑251 (MQ=255)                                                                                                                                                                                                           aaaaCACCAGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATc                             >  1:299748/1‑249 (MQ=255)                                                                                                                                                                                                                   aGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCt                                  <  2:119862/236‑1 (MQ=255)                                                                                                                                                                                                                   aGCGACGCGCCAGCCATTCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCt                                  >  1:119862/1‑236 (MQ=255)                                                                                                                                                                                                                              agccaTTCCCTCTGTTGCGCTGCTAGAACTCACCTGAACCCCGGTCGATCAATCTACCACCCACACCACCCAACTGGCGACCGGCCGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGCACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCct        >  2:24316/1‑251 (MQ=255)                                                                                                                                                                                                                                    tCCATCTGTTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTcc  >  1:130296/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   AAAACAGGTTGTCATTATCTGAAAGGGGCGAAAGCCCCTCTGATTATCGGGTTTAGCGCGCTATTGCCTGGCTACCGCTGAGCTCCAGATTTTGAGGTGAAAACAATGAAAATGAATAAAAGTCTCATCGTCCTCTGTTTATCAGCAGGGTTACTGGCAAGCGCGCCTGGAATTAGCCTTGCCGATGTTAACTACGTACCGCAAAACACCAGCGACGCGCCAGCCATTCCATCTGCTGCGCTGCAACAACTCACCTGGACACCGGTCGATCAATCTAAAACCCAGACCACCCAACTGGCGACCGGCGGCCAACAACTGAACGTTCCCGGCATCAGTGGTCCGGTTGCTGCGTACAGCGTCCCGGCAAACATTGGCGAACTGACCCTGACGCTGACCAGCGAAGTGAACAAACAAACCAGCGTTTTTGCGCCGAACGTGCTGATTCTTGATCAGAACATGACCCCATCAGCCTTCTTCC  >  NC_000913/4249449‑4249926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑