Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,084,932	G→T	F297L (TTC→TTA)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,084,932	0	G	T	100.0%	142.2 / NA	43	F297L (TTC→TTA)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/22);  total (21/22)

AGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATG  >  NC_000913/4084685‑4085166                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           aGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTTGTATCGCGTTTGGCTAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAAGCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTGCGGTGTAGTCGCGGTTTAAt                                                                                                                                                                                                                                         <  2:256620/251‑1 (MQ=255)   aTGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCAGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAAttt                                                                                                                                                                                                                                       <  1:244597/251‑1 (MQ=255)             ccACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTc                                                                                                                                                                                                                             <  1:114773/251‑1 (MQ=255)               acaTCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTc                                                                                                                                                                                                                             >  1:86639/1‑249 (MQ=255)                     ggCGGGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTGCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTcagcag                                                                                                                                                                                                                        <  2:13252/248‑1 (MQ=255)                        gtgtAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGGATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTAc                                                                                                                                                                                                                    >  2:168117/1‑249 (MQ=255)                                 gAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGcc                                                                                                                                                                                                         <  1:91096/251‑1 (MQ=255)                                                                 gcgcGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGTCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACgga                                                                                                                                                                         <  1:158102/251‑3 (MQ=255)                                                                         gTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGc                                                                                                                                                                  >  2:74897/1‑250 (MQ=255)                                                                            gCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGAGCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtag                                                                                                                                                              <  2:133022/251‑1 (MQ=255)                                                                                cGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATagtagt                                                                                                                                                          >  2:77413/1‑251 (MQ=255)                                                                                   ggTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCa                                                                                                                                                        <  1:4997/250‑1 (MQ=255)                                                                                    gTATCTCGTTTGGCGAAGCCTTGTTCGGCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTGTTCCGCGTCGTAGCCGGAGAACAGGCCATCTTCAAAGCCGTAATCCGCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCAGTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGc                                                                                                                                                      <  2:297757/251‑1 (MQ=255)                                                                                      aTCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAAGGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCt                                                                                                                                                     <  2:276172/250‑1 (MQ=255)                                                                                              tGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgccgcc                                                                                                                                            >  1:212617/1‑251 (MQ=255)                                                                                                  gAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAc                                                                                                                                        >  1:234767/1‑251 (MQ=255)                                                                                                   aaGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAcg                                                                                                                                       <  2:220643/251‑1 (MQ=255)                                                                                                      ccGTTTTCGTCCAGTTCATAAGTCGAGAAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTGGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCTTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGt                                                                                                                                    <  2:268924/251‑1 (MQ=255)                                                                                                         ttttCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTACGCGTCGTAGCCGGTGAACAGGCCATCTTCACAGCCGTAATCCTCACGCACGCTCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTaa                                                                                                                                  >  2:282388/1‑250 (MQ=255)                                                                                                              gTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTACGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGCCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCg                                                                                                                              >  1:18634/1‑249 (MQ=255)                                                                                                                 cAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgat                                                                                                                         <  1:12459/251‑1 (MQ=255)                                                                                                                     tCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGTGCATATAATTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatcga                                                                                                                      <  1:294315/250‑1 (MQ=255)                                                                                                                              cAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATc                                                                                                              >  1:22758/1‑249 (MQ=255)                                                                                                                              cAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATAGATCACAATc                                                                                                              >  1:270418/1‑249 (MQ=255)                                                                                                                                     aTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTcgcg                                                                                                     >  2:92230/1‑251 (MQ=255)                                                                                                                                     aTTTAACGTACTTGCGTTTTTCCGCGTCGGAGCCGGAGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTCATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTcgcg                                                                                                     >  2:181985/1‑251 (MQ=255)                                                                                                                                         aTCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCg                                                                                                  <  1:72689/250‑1 (MQ=255)                                                                                                                                           cGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACCGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCACTACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTtg                                                                                               >  1:115145/1‑251 (MQ=255)                                                                                                                                              aCTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTtgtg                                                                                             >  2:302887/1‑250 (MQ=255)                                                                                                                                                     tttttCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACTGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTgg                                                                                     >  2:210251/1‑251 (MQ=255)                                                                                                                                                              gtcgtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCg                                                                            <  1:1527/251‑1 (MQ=255)                                                                                                                                                                cgtAGCCGGGGGACTGGCCATCGTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTGTTCGCGGTTTAATTTTTCATTGTTCAGCAGGTTCAGCAAGACGCCTGACATGAAAGCAATTTCAGTACGGTAACGAATAGGGGCATAGTAGTCAGCTACTGCCGCCGTTCGCGTAAAGCTAGGATCGATCTGAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGcc                                                                          <  1:171544/251‑1 (MQ=255)                                                                                                                                                                 gtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGccc                                                                         >  1:73496/1‑251 (MQ=255)                                                                                                                                                                      cGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCgg                                                                    >  2:73928/1‑251 (MQ=255)                                                                                                                                                                            aCAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCATTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCg                                                              <  1:71327/251‑1 (MQ=255)                                                                                                                                                                             cAGGCCATCTTCAAAGCCGTAATCCTCACGCACGTTCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTCCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGAGCGCAATCAGCTTCGCGCCGTTGGGAATTTTGGCTTCCATCGCCCAGCGGAACCCGa                                                             <  2:137274/251‑1 (MQ=255)                                                                                                                                                                                                         cgcacgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGAACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGcc                                 >  1:25344/1‑251 (MQ=255)                                                                                                                                                                                                              gATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGTGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATc                             >  1:116328/1‑250 (MQ=255)                                                                                                                                                                                                                 cAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATcacc                          <  1:222533/250‑1 (MQ=255)                                                                                                                                                                                                                  aGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAAAGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATcaccac                        >  1:130923/1‑251 (MQ=255)                                                                                                                                                                                                                        gCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGGACAGCAATACGCCGGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATATTAGTCAGCGACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTGCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAGCCCGACCTGGTGAGCTTCAGCGGCGTTTTCGCACATGACCACGACGAg                  <  2:187207/251‑1 (MQ=255)                                                                                                                                                                                                                             ggTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCACTGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTgg             >  2:150914/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATg  <  1:92230/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           AGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGTTGGCGTTCTTGATG  >  NC_000913/4084685‑4085166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑