Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	881,531	A→T	I269F (ATC→TTC)	dacC →	D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	881,531	0	A	T	100.0%	105.2 / NA	31	I269F (ATC→TTC)	dacC	D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/17);  total (14/17)

CATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTGA  >  NC_000913/881289‑881762                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        cATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGtttt                                                                                                                                                                                                                                 >  1:173626/1‑251 (MQ=255)   ttGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGACGACAGGAACCACGGCAGGCGCGGGCTATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTCGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTa                                                                                                                                                                                                                                >  2:27499/1‑250 (MQ=255)    tGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAACGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAAt                                                                                                                                                                                                                              >  1:94497/1‑251 (MQ=255)     gCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAAt                                                                                                                                                                                                                              <  1:167331/250‑1 (MQ=255)        gggTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGTGCGATATGCGTTTAATCTCCGTAGTGTTTGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGt                                                                                                                                                                                                                          <  1:115990/251‑1 (MQ=255)             aaGCATTGCTCCACGATCTGCCGGCCGAGTACGCCATTCATAAAGAGAAAGAGTTCACCATCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGCACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGGAGTGTTGGGGGGGAAAACCGACCGCTTCCGTTTTAATGAGTCTg                                                                                                                                                                                                                       >  2:293316/1‑249 (MQ=255)                                                aTTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATTTCATGAAGACAGGAACCACTGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGTGCGATATGCGTTTAATCTCCGTATTGTTGGGGGCGAGAACCGACCGTTTCCGTTTTAAGGAGTCTGAGAAATTATTTACCTGGGGTTTCCGTTTCTTTGaacc                                                                                                                                                                                  <  2:27281/251‑3 (MQ=255)                                                       aagagaaagagTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACg                                                                                                                                                                           <  1:11011/251‑1 (MQ=255)                                                          agaaagagTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGccc                                                                                                                                                                        <  1:296650/251‑2 (MQ=255)                                                           gaaagagTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCaa                                                                                                                                                                       <  2:236556/251‑1 (MQ=255)                                                            aaagagTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAAt                                                                                                                                                                      >  1:191624/1‑251 (MQ=255)                                                              agagTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTa                                                                                                                                                                    <  1:175161/251‑1 (MQ=255)                                                                          caacaaAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGcc                                                                                                                                                         >  1:99176/1‑250 (MQ=255)                                                                                            aaaccgtaaccgtCTGCTGGGGAGCAGCAATCTGAATGTTGAGGGCATGAATACAGGAACCACGGCAGGCGAGGGAGATAATCTTGTTGCTGCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGGTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGGACTGGGGTTTCCGCTTCTTGGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGcgcg                                                                                                                                      <  1:12514/250‑1 (MQ=255)                                                                                                                                                 cAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCa                                                                                 <  1:209335/251‑1 (MQ=255)                                                                                                                                                     aaCCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTg                                                                              >  1:61458/1‑250 (MQ=255)                                                                                                                                                      aCCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGATCAGTGAc                                                                            >  1:277000/1‑251 (MQ=255)                                                                                                                                                                         ataATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCt                                                          <  1:59533/250‑1 (MQ=255)                                                                                                                                                                         ataATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTg                                                         >  1:116285/1‑251 (MQ=255)                                                                                                                                                                               tGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACcgcg                                                                   >  1:224811/1‑235 (MQ=255)                                                                                                                                                                               tGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACcgcg                                                                   <  2:224811/235‑1 (MQ=255)                                                                                                                                                                                    gCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGa                                               <  1:291159/250‑1 (MQ=255)                                                                                                                                                                                              cccAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGaaa                                                                                                                                                                                                                  >  1:128461/1‑77 (MQ=255)                                                                                                                                                                                              cccAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGaaa                                                                                                                                                                                                                  <  2:128461/77‑1 (MQ=255)                                                                                                                                                                                                           gCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAAGCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAAc                       <  2:61458/251‑1 (MQ=255)                                                                                                                                                                                                             gTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTtgtg                                                                                                                                                <  1:34015/128‑1 (MQ=255)                                                                                                                                                                                                             gTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTtgtg                                                                                                                                                >  2:34015/1‑128 (MQ=255)                                                                                                                                                                                                                    ctcCGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCtt               >  2:121523/1‑250 (MQ=255)                                                                                                                                                                                                                      ccGTAGTGTTGGGGGCGAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTacc            <  2:12171/251‑1 (MQ=255)                                                                                                                                                                                                                                      gAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTGa  <  2:175195/245‑1 (MQ=255)                                                                                                                                                                                                                                      gAAAACCGACCGTTTCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTGa  >  1:175195/1‑245 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        CATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAAGAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAGCTTACCGCACCGCTGA  >  NC_000913/881289‑881762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑