Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,894	C→T	G37E (GGG→GAG)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,894	0	C	T	100.0%	108.2 / NA	33	G37E (GGG→GAG)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/16);  total (17/16)

GTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTA  >  NC_000913/3926653‑3927122                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      gTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AAGGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGa                                                                                                                                                                                                                              <  1:148276/251‑1 (MQ=255)  tcgatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGAc                                                                                                                                                                                                                             >  1:34753/1‑251 (MQ=255)     atcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATCGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGcc                                                                                                                                                                                                                          >  1:69268/1‑251 (MQ=255)                             aaGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGt                                                                                                                                                                                                  >  1:69557/1‑250 (MQ=255)                              aGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTa                                                                                                                                                                                                 >  1:211836/1‑250 (MQ=255)                               aaTGCTGGAGAGGCCGGTTGTGTGATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTGAGCCAGCGCGGAAGGGGAGTCTTTGGAGCTCTTTAATATAATGCCGATAAAGCAGCCTACGGCATAACCGAGCTGCTTCGGGCTGACATCCATAAGCGCCCCGGT‑AATGATCCCCGCCGGCTTCATTTTCTCTACGCGAACGTGAAGCGTCTCCGGACTGACGCCACAGTTGTT‑CGCCCGTGCGGCGTaa                                                                                                                                                                                                >  2:82205/2‑250 (MQ=255)                                  gCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AAGGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCg                                                                                                                                                                                             <  1:203248/250‑1 (MQ=255)                                                         aGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGGAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCt                                                                                                                                                                     >  2:54482/1‑251 (MQ=255)                                                                       tcatcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACTTCAATACGCGCCCCGTT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGGGCGCGCATTGCCCATTAATGCTTCCAGGATGCCa                                                                                                                                                         <  2:292552/249‑1 (MQ=255)                                                                            aaGGCTTTCCATCTTTGCCAGCGCGGAAGGGTAGTCTTGGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAAGACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACTCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCa                                                                                                                                                  <  1:22181/251‑1 (MQ=255)                                                                                    ccAGCTTTGCAAGCGAGGAAGGGTTGCCGTTGGCGCTCTTTAATATAACGCGAATAAAGCAGCTTAGTTCATACCGGGGCGGAGTCGGGCTGACATAAATCCGC‑CACCGGTTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCAGTAAGGCTTCCAGGATGCCACGGTCCGGATTGTc                                                                                                                                           <  2:297069/250‑1 (MQ=255)                                                                                                            agtttttGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGTTGCTTCGGGCTGACATCAATCCGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGAtt                                                                                                                   <  2:59535/246‑1 (MQ=255)                                                                                                                          tttaagttaaggCCGATAAAGCATCCCACGTCACAACCGAGCTGCTTCGGGCTGACGTCAATCCGCGCCCCGGT‑AATGACCCCCGCCTGCTTCATTTTCTCATCTCGAACGTGAAGCGTCTCCGGACTGACGTCA‑AATTGTTTCGCCAGTTCGGCGGAAGCGGTGCGCGCATTGCCCATTAAGGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGAtt                                                                                                     <  1:199613/239‑1 (MQ=255)                                                                                                                           ttAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCCTAGGATTTTCTTATGCGGATtg                                                                                                    >  2:92642/1‑250 (MQ=255)                                                                                                                              atatAATGCAGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCATGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCAATAGGCTTTTCTTATGCGGATtgatg                                                                                                 >  2:157767/1‑250 (MQ=255)                                                                                                                                        gATAAAGCAGCCTCCGTCATAACCGGGCTTGTTCGGGCTGACATCAATCCGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTc                                                                                                                                                                   <  2:117911/174‑1 (MQ=255)                                                                                                                                        gATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTc                                                                                                                                                                   >  1:117911/1‑174 (MQ=255)                                                                                                                                                   cTACGTCATAACCGGGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTGCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGcc                                                                                                                                                          <  2:286179/172‑1 (MQ=255)                                                                                                                                                   cTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATAAGCGCCCCGGT‑CATGATCCCCGCCTGCTTCATTTTCTCTACTCGCACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTACGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGcc                                                                                                                                                          >  1:286179/1‑172 (MQ=255)                                                                                                                                                               cGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAAt                                                               >  1:178394/1‑251 (MQ=255)                                                                                                                                                                gggctgctTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATAGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCt                                                                                        <  2:213493/223‑1 (MQ=255)                                                                                                                                                                gAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCt                                                                                        >  1:213493/1‑225 (MQ=255)                                                                                                                                                                                   tCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATACATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCtttt                                           >  2:249740/1‑251 (MQ=255)                                                                                                                                                                                                cGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTTAATGatta                              <  1:264367/251‑1 (MQ=255)                                                                                                                                                                                                          tCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCAtgtg                     >  2:247144/1‑251 (MQ=255)                                                                                                                                                                                                                ccTGCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTGTAATGAAGCAAAAGTGAGTTAGGCTTGTTATTGAATGATTATTGCATGTGTGTCgg               <  2:1595/251‑1 (MQ=255)                                                                                                                                                                                                                   gCTTCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGttt            >  2:34714/1‑251 (MQ=255)                                                                                                                                                                                                                      tCATTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGttttt          <  2:211836/250‑1 (MQ=255)                                                                                                                                                                                                                        aTTTTCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTttgtt       >  1:279426/1‑251 (MQ=255)                                                                                                                                                                                                                           ttCTCTACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATACATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGc     <  1:249740/250‑1 (MQ=255)                                                                                                                                                                                                                               ctACTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGTCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTa  <  1:157767/249‑1 (MQ=255)                                                                                                                                                                                                                                 aCTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGa                                                                                                                     <  2:215897/132‑1 (MQ=255)                                                                                                                                                                                                                                 aCTCGAACGTGAATCGTCTCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGa                                                                                                                     >  1:215897/1‑132 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      GTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGT‑AATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCA‑AATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTA  >  NC_000913/3926653‑3927122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑