Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,904,907	C→T	E231K (GAA→AAA)	bglF ←	beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,904,907	0	C	T	100.0%	93.5 / NA	29	E231K (GAA→AAA)	bglF	beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

CAGGCCCCAGTGCAGTCCGAACATGACGAAGATTTGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGA  >  NC_000913/3904671‑3905134                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    cAGGCCCCAGTGCAGTCCGAACATGACGAAGATTTGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGcac                                                                                                                                                                                                                        >  2:289887/1‑250 (MQ=255)         aGTGCAGTCCGAACATGACGAAGATTTGCCAGAAGCCGCCCATTCCCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGct                                                                                                                                                                                                               <  2:171431/251‑2 (MQ=255)           tGCAGTCCGAACATGACGAAGATTTGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGCCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATCGCAATGGCGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGc                                                                                                                                                                                                                >  2:266847/1‑248 (MQ=255)                  cGAACATGACGAAGATTTGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAaata                                                                                                                                                                                                      <  1:115175/251‑1 (MQ=255)                            gAAGATTTGCCAGAAGCCGCCCATTACCGCGCCCGCAACTGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGCGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATa                                                                                                                                                                                             >  1:289762/1‑250 (MQ=255)                              aGATTTGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAAcc                                                                                                                                                                                          <  1:151369/251‑1 (MQ=255)                                aTTTGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCCGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCg                                                                                                                                                                                         >  1:300631/1‑250 (MQ=255)                                 tttGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCCATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGAt                                                                                                                                                                                       >  2:22779/1‑251 (MQ=255)                                               cccATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTca                                                                                                                                                                          >  1:100018/1‑250 (MQ=255)                                                                                                                     ttATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCGGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTc                                                                                                   >  1:12394/1‑251 (MQ=255)                                                                                                                             gTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTa                                                                                            >  1:161188/1‑250 (MQ=255)                                                                                                                               tGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGGAATTCAACAATGTGACCGGAAGACCCAGGAAATCCAGCCCCAGCGGATCCGCTTTTTGCCCGGTCGCGAAAGCAGTCAGAATTAAt                                                                                          <  2:27213/250‑1 (MQ=255)                                                                                                                                             ccaGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCt                                                                           <  1:289887/251‑1 (MQ=255)                                                                                                                                               agcagAAAGGTGACGGGTGAGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTACCCACGCATTAAGTCGGCGTTTAAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGCGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCACCAGCGCATCCGCTTTTTGCCAGATCTCGAAAGCAGACAGAATTAATGGATGCACTAACGCTcc                                                                         >  2:159679/1‑251 (MQ=255)                                                                                                                                                  agAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTccacc                                                                      >  2:155822/1‑251 (MQ=255)                                                                                                                                                      aGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAACAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATc                                                                  <  2:128920/251‑1 (MQ=255)                                                                                                                                                           aCGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGACTAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTACCGCTCCACCAATCACCAt                                                             >  2:68330/1‑251 (MQ=255)                                                                                                                                                                    aTAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCCCCATGGCAGTaaa                                                    <  2:27611/251‑1 (MQ=255)                                                                                                                                                                        ccATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGa                                                <  1:22779/251‑1 (MQ=255)                                                                                                                                                                              gACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTATCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAAAGCTCCACCAATCACCATGGCAGTAAATGGATTAccg                                          >  2:6653/1‑251 (MQ=255)                                                                                                                                                                               aCATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCtttt                                                                                                                        <  2:160412/172‑1 (MQ=255)                                                                                                                                                                               aCATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCtttt                                                                                                                        >  1:160412/1‑172 (MQ=255)                                                                                                                                                                                        gggTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTGTTGCCCGTGCTCGAAAGCAGTCAGAATTAATGGATTCACGAACGCTCCACCAATCACCTTGGCAGTAATTGTATTACCGCCGAAGCGtt                                <  2:289762/250‑1 (MQ=255)                                                                                                                                                                                             ggaagaaATTGTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTccac                           <  2:12394/250‑3 (MQ=255)                                                                                                                                                                                                              gCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCCCCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGAt          <  1:252017/251‑1 (MQ=255)                                                                                                                                                                                                              gCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGAt          <  1:189332/251‑1 (MQ=255)                                                                                                                                                                                                              gCCGACGGTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCAGCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCTCCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGAt          <  2:189487/251‑1 (MQ=255)                                                                                                                                                                                                                    ggTAACCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCgg    >  2:159645/1‑251 (MQ=255)                                                                                                                                                                                                                       aaCCACGCATTAAGTCGGCGTTTCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGga  <  2:164479/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    CAGGCCCCAGTGCAGTCCGAACATGACGAAGATTTGCCAGAAGCCGCCCATTACCGCGCCCGCAAATGCAGGAACCGCCTGATAAAGCCAGAGATAACCGGCGGCAATCAGTTCGCTTATCCAGGTTGATAGCGGCCCCACCAGCAGAAAGGTGACGGGTGTGATAACCATCAGACATAGCAATGGTGTGAAGAAATTTTTGATTGCCGACGGTAACCACGCATTAAGTCGGCGTTCCAGAATGCTGCACAACCAGGCAGAAAAAATAATGGGAATAACCGATGACGAGTAATTCAACAATGTGACCGGAATACCCAGGAAATCCAGCCCCAGCGCATCCGCTTTTTGCCCGTTCTCGAAAGCAGTCAGAATTAATGGATGCACTAACGCTCCACCAATCACCATGGCAGTAAATGGATTACCGCCGAAGCGTTTCCCCGCGGTGTATCCCAGGATTATCGGGA  >  NC_000913/3904671‑3905134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑