Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,507	C→T	A74T (GCG→ACG)	uhpA ←	DNA‑binding transcriptional activator UhpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,507	0	C	T	100.0%	106.6 / NA	35	A74T (GCG→ACG)	uhpA	DNA‑binding transcriptional activator UhpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/22);  total (13/22)

CCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAA  >  NC_000913/3850264‑3850748                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ccGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCt                                                                                                                                                                                                                                            <  1:12763/251‑1 (MQ=255) ccGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCt                                                                                                                                                                                                                                            <  1:45791/251‑1 (MQ=255)         ccTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGc                                                                                                                                                                                                                                      >  2:300994/1‑249 (MQ=255)                        tCCGCCACCGGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCt                                                                                                                                                                                                                      <  1:175113/250‑1 (MQ=255)                                   gCGTTCACGGTTGGTTAGCTGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCAGCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCAGACCTTTCTGCAGCTGGCTTAGCAGCTCCAGACCGgaga                                                                                                                                                                                                          <  2:293920/251‑1 (MQ=255)                                               ggTTAGCGGGTCCTGACGACCGGATGCCAGCTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATgg                                                                                                                                                                                              >  2:127856/1‑251 (MQ=255)                                                       ggTCCGGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATTGAGATATCg                                                                                                                                                                                      <  2:51191/251‑1 (MQ=255)                                                             gacgacCGGATGCCAGTTTAATTGCAATATCCGGCGTTAGATAACAGCCGCCCTTGGTAACCGTATGCACCGCAGCAATGAGTTCATGCGGGCTACAGCGGTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTGGGCAGCTGGCTTAGCAGGTCCAGACCGGAGATATCGGGCATGGAGATATCGCaaa                                                                                                                                                                                  <  2:252585/249‑1 (MQ=255)                                                             gacgacCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAAt                                                                                                                                                                                 <  2:265111/250‑1 (MQ=255)                                                                  ccGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACCGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATACTCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATacacac                                                                                                                                                                           >  2:182168/1‑251 (MQ=255)                                                                            gTTTAATGGCAATCTCCGGCTTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAAGGAGTTCATCCGGGCTACCGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAAACAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGAAATAGGGCATGGAGATATCGCAAATACACCCCGGCACAccc                                                                                                                                                                 >  2:282788/1‑250 (MQ=255)                                                                                           ccGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGccc                                                                                                                                                   <  2:303053/250‑1 (MQ=255)                                                                                                        aCAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGACGCGTGCCCCCGCGTTCAGCGCCTGCTCAAACAGCGCAGGAATGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACAGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCTCCGCCAGCGCCTcgc                                                                                                                                     >  1:132410/1‑251 (MQ=255)                                                                                                           gccgccCGTGGCAACCGTCTGCCCCGCAGCAATGAGTTCATCCGGGCTACAGCGTGTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCGGCCAAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCGGGCTTAGCAGCTCCAGACCGGAGATAGCGGGCATGGAGATATCGCAAACACACCCCTGCACCCCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGcc                                                                                                                                  <  2:56470/251‑1 (MQ=255)                                                                                                                      cAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTa                                                                                                                                                                                                                            <  2:44606/150‑1 (MQ=255)                                                                                                                      cAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTa                                                                                                                                                                                                                            >  1:44606/1‑150 (MQ=255)                                                                                                                             aggCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCaa                                                                                                                <  1:87057/249‑1 (MQ=255)                                                                                                                                cACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACt                                                                                                              >  2:56539/1‑250 (MQ=255)                                                                                                                                 aCCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTAc                                                                                                            >  2:130154/1‑251 (MQ=255)                                                                                                                                         aaTGAGTTCATCCGGGCTTCAGCGTTGTGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCCGCGCAGGACTGTCGTGACCGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACCCCTCCACACCGCGCCCTGGCAGCTCCGCCAGCGCCTGGCGCCCCGAACCAAACTCGGCATCTACCTGCAAAt                                                                                                    <  1:175215/251‑1 (MQ=255)                                                                                                                                                 cATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTcc                                                                                            <  1:220704/251‑1 (MQ=255)                                                                                                                                                  aTCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGTAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAAATATCGGGCATGGATATATCGCAAATGCACACCTGCACGCCGCGCCCCGGCATCCCCGCCAGCGCCGCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCa                                                                                           <  1:299379/251‑1 (MQ=255)                                                                                                                                                     cGGGCTCCGGCGTTTGGAAAGAAAGCCGCGTGCCCCCTCGTTAAGCGCCTGCTCACCCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTGCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGcc                                                                                        <  2:204787/251‑1 (MQ=255)                                                                                                                                                                                   tGCCCCCGCGGTAAGCGCCTGCTCAACCACCGCAGGACTGTCGTGAACGGAGAGAATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATAGGGCATGGAGATATCGCAAATACACACCTGCACTCCGCGCCCCGGCACCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGAc                                                            <  1:282788/249‑1 (MQ=255)                                                                                                                                                                                       cccGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTcc                                                                                            >  1:25748/1‑213 (MQ=255)                                                                                                                                                                                               aaGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGt                                              >  1:301866/1‑251 (MQ=255)                                                                                                                                                                                                       gCTCAACCAGCGCAGGCCTGTCGTGAACGGAGATCATACGCGTCGTCATCCCTTCCGGCAGCGGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATACCGCAAATCCACCCCGGCCCACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAg                                       <  2:301866/250‑1 (MQ=255)                                                                                                                                                                                                       gCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAg                                       <  1:136733/250‑1 (MQ=255)                                                                                                                                                                                                                          gTCGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCCTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGCCGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCt                   >  1:25808/1‑251 (MQ=255)                                                                                                                                                                                                                           gcGTGAACGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCtt                  <  1:98701/250‑1 (MQ=255)                                                                                                                                                                                                                              ggacaggAGAGCATAAGCTTCGTCATGCCTTTCGCCAGCGGGCTTAGCAGCTCCAGACCGGAGATATCGGGCAGGGAGATATCGCAAATACCCACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTGCCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTc               <  2:87763/246‑1 (MQ=255)                                                                                                                                                                                                                                 aCGGAGAGCATAATCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGACTATAAGGGCAACGGTGATCATGGTCTTGTCCTg            >  1:28232/1‑251 (MQ=255)                                                                                                                                                                                                                                        gCATAATCGTCGTCATCCCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTa     <  1:56539/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaaTCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACCCACCTGCACCCCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTaaaa  <  1:130154/250‑1 (MQ=255)                                                                                                                                                                                                                                            aaTCGTCGTCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGCTGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTgaaa  >  2:117103/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAATGGCAATATCCGGCGTCAGATAACAGCCGCCCGTGGCAACCGTATGCACCGCAGCAATGAGTTCATCCGGGCTACAGCGTTTGGAAAGAAAGCCGCGTGCCCCCGCGTTAAGCGCCTGCTCAACCAGCGCAGGACTGTCGTGAACGGAGAGCATAATCGTCGCCATACCTTTCGGCAGCTGGCTTAGCAGCTCCAGACCGGAGATATCGGGCATGGAGATATCGCAAATACACACCTGCACACCGCGCCCCGGCAGCCCCGCCAGCGCCTCGCGCCCCGAACCAAACTCGGCAACTACCTGCAAATCAGGTTCCAGCCCCAGCAGCTGCGCAAAGCCGGAGCGGACGATGAGGTGATCGTCTATAAGGGCAACGGTGATCATGGTCTTGTCCTGGCGGGTAAAA  >  NC_000913/3850264‑3850748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑