Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,834,845	C→T	S362N (AGT→AAT)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,834,845	0	C	T	97.4%	129.8 / ‑6.9	39	S362N (AGT→AAT)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/23);  minor base G (1/0);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGT  >  NC_000913/3834599‑3835087                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   tGAGCCAGCTGTTCCATAAAGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGAt                                                                                                                                                                                                                                                >  2:188919/1‑251 (MQ=255)        gCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCCGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTc                                                                                                                                                                                                                                         >  1:74386/1‑251 (MQ=255)                  aaCGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGAAAAAAACAGATCGCGGGAACAATCGGGAATAGCGCAATAAGGATGCTAATCGGGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTa                                                                                                                                                                                                                                <  2:95209/250‑1 (MQ=255)                                 gtgCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGc                                                                                                                                                                                                                >  1:175778/1‑251 (MQ=255)                                       ggggagggagtGGTAGCGTTAAGCGATAACCGCGCTCAGCAAATAACAGATCGCCGAAACAATCGTGAATAGCGAAATAATGATGCTATTCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGAATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTg                                                                                                                                                                                                                          <  2:12275/228‑1 (MQ=255)                                       cgtGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTg                                                                                                                                                                                                                          >  1:12275/1‑235 (MQ=255)                                            gtgagtAGTAGCGTTTAGCGATAATCGCCCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                                                                                                                     <  1:112137/251‑1 (MQ=255)                                                    tagCGCTTAGCGATAATCGAGCTCAGCAAATAACAGATCGCCGGAACAAGCGTGACTAGCGCACTAATGATGCTAAGCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAACCAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCCACACTCGCCTAAGTCGACGGTATCgg                                                                                                                                                                                              >  2:147153/1‑250 (MQ=255)                                                              cGATAACCGCGCTCAGAAAATAACAGATCGCCGGAACAATCGTGAAAAGCGCAATAATGATGCTAATCGTGGCTC‑GTTCTTGCGCTTTTGCTGCCGCAGCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAGCCCCAGTTTGAGCACAAACAGCGGGCCAGCAAAATTGATCCCGGTCATGCGGTTTCCATTTCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAcc                                                                                                                                                                                   <  1:153205/251‑1 (MQ=255)                                                               gATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTAccc                                                                                                                                                                                  >  2:228163/1‑251 (MQ=255)                                                                 tAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGcc                                                                                                                                                                                                               >  1:148648/1‑220 (MQ=255)                                                                 tAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTCCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGcc                                                                                                                                                                                                               <  2:148648/220‑1 (MQ=255)                                                                 aaaccgcgCTCAGCAAATACCAGATCGCCGAAACAATCTTGAATAGCGCAATAATGATGCTAATGTTGGCTCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTGTACCAGTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCAc                                                                                                                                                                                <  1:142018/247‑1 (MQ=255)                                                                  aaTCGCGCTCAGAAAATAACAGATCGCCGGAACAATCGTGAATAGCACAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACt                                                                                                                                                                               <  1:272812/251‑1 (MQ=255)                                                                                       gATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCATGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGAt                                                                                                                                                          <  1:82449/251‑1 (MQ=255)                                                                                         cggCCGGAACAATCGTGAATAGCGCAATAATGTTGCTAATCGTGGCTCTGTTCT‑GCGCTTTTTCTGCGGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGc                                                                                                                                                        <  2:16217/249‑1 (MQ=255)                                                                                           gCCGGAAAAATCGTGAATAGCGCAATAATGATGCTAATGGTGGCGTGGTTCT‑TCGCTTTTTCGGCCGAATAATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGGTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGTCGTTTACCATTGCAGCACGCGCCGTAGTCGACGGTATCGTACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCaa                                                                                                                                                      <  1:142327/251‑1 (MQ=255)                                                                                               gAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGCGCGCCGCCGAAGGCCAACCCCAGCTTGAGCACAAACAGCGTGCCCGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacac                                                                                                                                                   >  1:27904/1‑250 (MQ=255)                                                                                                       gTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCAc                                                                                                                                          >  2:174525/1‑251 (MQ=255)                                                                                                            gagcgaaaaaatGATGCTAACCGGGCCCCTTTTCT‑GCGCTTTTCCGGCCGTACAATACCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTGTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGa                                                                                                                                     <  2:190203/242‑1 (MQ=255)                                                                                                                        gatgCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAAc                                                                                                                          >  1:36547/1‑250 (MQ=255)                                                                                                                             tAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTg                                                                                                                    >  1:112116/1‑251 (MQ=255)                                                                                                                              aaTCGTGGCGCTGTCCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAAGCACGAAGATTAAGACAAGCATAGt                                                                                                                     <  1:233196/249‑1 (MQ=255)                                                                                                                               aTCGTGGCGCTGATCT‑GCGCTTTTTCTTCCGCATCATATCAGCCATAAGCCAGCATCCAGCCGCTAAGAGCGCCGCCGACGGCCAAACCCAGCTTGCGCACATACAGCGTGCCAGCAAAAGTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTCGTCGACGGTATCGGACATCATTACCCACTGGATAGGGGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACCTAGTGAt                                                                                                                  >  2:184329/1‑251 (MQ=255)                                                                                                                                                  gcTTTTTCTGCCACATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAAc                                                                                                <  1:160989/251‑1 (MQ=255)                                                                                                                                                  gcTTTTTCGGCCGCACCATTTCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGTCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAAc                                                                                                <  1:57520/251‑1 (MQ=255)                                                                                                                                                       ttCTGCCTCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAg                                                                                            <  1:130490/250‑1 (MQ=255)                                                                                                                                                         cgcccacaccatATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGaa                                                                                          <  2:140080/241‑1 (MQ=255)                                                                                                                                                                     aaccGCCATAAGCCAGCAGCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACGCCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCt                                                                              <  2:137119/248‑1 (MQ=255)                                                                                                                                                                          ccATAAGCCAGCATCCCGCCGATACGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCAc                                                                        >  1:91655/1‑251 (MQ=255)                                                                                                                                                                               aGCCAGCATCCAGACGATAAGAGCGCCGCCGCAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGcc                                                                    >  2:47858/1‑250 (MQ=255)                                                                                                                                                                                gCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCcat                                                                  <  2:247077/251‑2 (MQ=255)                                                                                                                                                                                           gCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCg                                                       <  2:175778/251‑1 (MQ=255)                                                                                                                                                                                                  agagCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTccacca                                                <  1:36763/251‑1 (MQ=255)                                                                                                                                                                                                   gagCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTccacc                                                 >  1:288498/1‑249 (MQ=255)                                                                                                                                                                                                           ccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGt                                       >  1:203097/1‑251 (MQ=255)                                                                                                                                                                                                                       cccAGTTTGAGCACAAACAGCGTGCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACAttt                           <  1:108272/251‑1 (MQ=255)                                                                                                                                                                                                                                              gCCAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCa    <  1:265019/251‑1 (MQ=255)                                                                                                                                                                                                                                                cAGCAAAATTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGt  <  1:247549/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   TGAGCCAGCTGTTCCATAACGGTTTTCAGATTGTGCGTCGTGAGTGAGTAGTAGCGTTTAGCGATAATCGCGCTCAGCAAATAACAGATCGCCGGAACAATCGTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCT‑GCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGT  >  NC_000913/3834599‑3835087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑