Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,803,945	C→T	G65S (GGT→AGT)	waaB ←	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,803,945	0	C	T	100.0%	79.7 / NA	25	G65S (GGT→AGT)	waaB	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

GCCAGAGCTGATTGCTAAATGATAGTCGGCACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTA  >  NC_000913/3803715‑3804191                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gCCAGAGCTGAATGCTAAATGATAGTCGACACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAAAAAAATGAGGCCATGAAAATACTGGCATATCAATTCCGGATTTTTTACGTGCATTTGCGGCAAACAGGCAGGAGAGCACATCAATACAAATAACGATATCGGGTTGGTACTCATGAAGCCATGTACTTAGAGCATGAAAATGGGACGCCCGACGCAAAAAACTTAAACGAATATTGTAAAATg                                                                                                                                                                                                                                    <  2:268963/251‑1 (MQ=255)    agagCTGATTGCTAAATGATAGTCGGCACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAAc                                                                                                                                                                                                                                 <  2:97925/251‑1 (MQ=255)                                   gTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCtt                                                                                                                                                                                                  >  2:62254/1‑251 (MQ=255)                                    tGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCtt                                                                                                                                                                                                  >  2:41101/1‑250 (MQ=255)                                                                 aaaGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGaaa                                                                                                                                                                      <  1:20863/249‑1 (MQ=255)                                                                     aaaaaTGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACAtt                                                                                                                                                                <  1:288999/251‑1 (MQ=255)                                                                          tgtgGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACt                                                                                                                                                           >  2:285732/1‑251 (MQ=255)                                                                                   gAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTGCAGCCATCCCTTAGCCATTTTTTCAGTTCGACAAAAGAAAAACATTTCACTTTGGATAtg                                                                                                                                                  <  1:188703/251‑1 (MQ=255)                                                                                   gAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATAtg                                                                                                                                                  >  2:223721/1‑251 (MQ=255)                                                                                     aaaTACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATAtgtt                                                                                                                                                <  1:223721/251‑1 (MQ=255)                                                                                                                          tttGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGt                                                                                                           >  1:48273/1‑251 (MQ=255)                                                                                                                              cGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTGGTCATTACGACAAAAGAAAAACATTTCACTTTGGAGATGTTGTTTCCTGAAGGTAGTAATAACATCACGGATGACAGTTTcc                                                                                                       <  1:203309/251‑1 (MQ=255)                                                                                                                                                tCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTACCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGgt                                                                                                                                   <  2:119964/205‑1 (MQ=255)                                                                                                                                                tCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTACCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGgt                                                                                                                                   >  1:119964/1‑205 (MQ=255)                                                                                                                                                   caTCAATACAAATAGCGATATCTGGTTGGTAATCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCACGAAGCAAAAAACTTAAACGCATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGCCAGTTTCCATGCCGCCAAATCCAGacac                                                                                   >  2:120962/1‑247 (MQ=255)                                                                                                                                                   caTCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAAc                                                                                   >  2:258519/1‑250 (MQ=255)                                                                                                                                                         tACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCg                                                                            >  1:19628/1‑251 (MQ=255)                                                                                                                                                                    atatCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGc                                                                 >  2:199664/1‑251 (MQ=255)                                                                                                                                                                              ggtACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATa                                                       <  1:258519/251‑1 (MQ=255)                                                                                                                                                                                           ccATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTa                                          <  2:27163/251‑1 (MQ=255)                                                                                                                                                                                                 aCTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTg                                      >  2:167209/1‑249 (MQ=255)                                                                                                                                                                                                          ataaaTATGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCAAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAGAACTCa                           <  2:231567/248‑1 (MQ=255)                                                                                                                                                                                                              atatGTTTCGCCCGACGCAAAAAACTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGt                       <  2:19628/251‑1 (MQ=255)                                                                                                                                                                                                                             cGCAAAAAACTTAAACGAATATTGGAAAATTAACATGAGTATTTAATACCGTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCCCTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTACTTGAGTAAAACTCACTGTTTTAACGGTTTTAtt        <  2:132430/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaCTTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGCCAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATGTTCATAGAAACTCTCATGAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCta  <  1:22745/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GCCAGAGCTGATTGCTAAATGATAGTCGGCACAGGTGATATATTCAGCATGTTTTTTGTGATCTAAAGAAAAATGTGGCCATGAAAATACTGGCATATCAATTCCTGATTTTTTACGTGCTTTTGCGGCAAACAGGCAGGAGATCACATCAATACAAATAACGATATCTGGTTGGTACTCCTGAAGCCATTTACTTAGAGCATGAATATGTTTCGCCCGACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTA  >  NC_000913/3803715‑3804191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑