Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,803,453	C→T	E229K (GAA→AAA)	waaB ←	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,803,453	0	C	T	100.0%	101.0 / NA	31	E229K (GAA→AAA)	waaB	UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

GGTTGATACAGATGGCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTCTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTACACCTCG  >  NC_000913/3803221‑3803693                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 ggTTGATACAGATGGCCATTGACATCCGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGc                                                                                                                                                                                                                                 >  1:235371/1‑250 (MQ=255)     gATACAGATGGCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCAtttt                                                                                                                                                                                                                            <  1:18621/251‑1 (MQ=255)              ggCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGTGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATc                                                                                                                                                                                                                    <  1:293988/250‑1 (MQ=255)              ggCCATTGACATCAGGTTGAATAATGTCAGCAGGACCGGAGCCACAATCGACACTGATACAAGGAATCCCCCACGAAAAGACTTCAAGTAGGGTCATTGGGAAACCTTCAAACGATGACGTGAGCAATAAAGCACTCACTTTTTCAATATATTGCTGGACCAATTCCCAGGGATGTTGTTGCAAACCATACCAGACAATTCGATCATCACTATTTAATTTTCTACCATAAGCCTGGAATTTTTCGAAATCg                                                                                                                                                                                                                   >  1:283878/1‑251 (MQ=255)                             gTTGAATAATGAAAGCAGGCCCGGCCACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTAATTGGGAAACCTTCAAAAGCTGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCCGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCCATATTTACTTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAAt                                                                                                                                                                                                    >  2:22745/1‑251 (MQ=255)                                                   ggAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCtt                                                                                                                                                                              >  1:145245/1‑251 (MQ=255)                                                         acaATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCtt                                                                                                                                                                        <  2:13680/251‑1 (MQ=255)                                                               ggCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGAt                                                                                                                                                                   >  1:231567/1‑250 (MQ=255)                                                                                     cACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAAc                                                                                                                                            <  2:235371/251‑1 (MQ=255)                                                                                     cACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACAGGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCAGCAAGCAGTTCTTTAAc                                                                                                                                            <  2:149737/251‑1 (MQ=255)                                                                                              gCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTg                                                                                                                                   >  1:19319/1‑251 (MQ=255)                                                                                                                gggAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATAc                                                                                                                  <  2:145245/250‑1 (MQ=255)                                                                                                                  gAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGcc                                                                                                               <  1:292571/251‑1 (MQ=255)                                                                                                                           aaaaGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTg                                                                                                                                   >  2:198330/1‑222 (MQ=255)                                                                                                                                             aaaCGCACTCAATTTTTCCATATCTTGCTGGACCAATTACCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCAc                                                                                    >  2:203309/2‑251 (MQ=255)                                                                                                                                               aCGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCAcc                                                                                   >  1:80818/1‑250 (MQ=255)                                                                                                                                               aCGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCAcc                                                                                   <  2:80818/250‑1 (MQ=255)                                                                                                                                                     tcactTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCg                                                                            >  2:20863/1‑251 (MQ=255)                                                                                                                                                             tCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCgg                                                                     <  1:240176/250‑1 (MQ=255)                                                                                                                                                                         tGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTc                                                        >  2:189174/1‑251 (MQ=255)                                                                                                                                                                             ccAATTCCCAGGGATATTGGTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCGGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTa                                                    <  2:237466/251‑1 (MQ=255)                                                                                                                                                                                      aGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACg                                           <  1:286982/251‑1 (MQ=255)                                                                                                                                                                                                   ccAACCATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATa                               >  1:132430/1‑250 (MQ=255)                                                                                                                                                                                                        cATACCAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACAttt                         >  2:84252/1‑251 (MQ=255)                                                                                                                                                                                                            ccAGACAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTAtcg                     >  2:56441/1‑251 (MQ=255)                                                                                                                                                                                                                aCAATTCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTAtcgtcg                  >  1:97925/1‑250 (MQ=255)                                                                                                                                                                                                                     tCGATCATCAATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCt             <  1:189174/250‑1 (MQ=255)                                                                                                                                                                                                                          catcaATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTCGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTac        >  1:91817/1‑250 (MQ=255)                                                                                                                                                                                                                           atcaATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTacac      >  1:70539/1‑251 (MQ=255)                                                                                                                                                                                                                             caATATTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTACAcc     <  1:56441/250‑1 (MQ=255)                                                                                                                                                                                                                               atatTTAATTTTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAAGACATGGAGTTTCCAGTTACCTGTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTTAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTAGCGTCGATTCTGCTACACCTCg  <  2:137481/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 GGTTGATACAGATGGCCATTGACATCAGGTTGAATAATGTCAGCAGGCCCGGAGACACAATCGGCACTGATACAAGGAATCCCCCACGACAAGGCTTCAAGTAGGGTCATTGGGAAACCTTCAAAAGATGACGTGAGCAATAACGCACTCACTTTTTCAATATCTTGCTGGACCAATTCCCAGGGATATTGTTGCCAACCATACCAGACAATTCGATCATCAATATTTAATTCTCTACCATAAGCCTGGCATTTTTCGAAATCGGAGCCATCACCCAATACATGGAGTTTCCAGTTACCTTTCGCTTGTGATAAGCCATCAAGCAGGTCTTTAACTCTTTTCTGGCCTTCAAATTTCATACGCCCAACATAAATAAACGTTGCCGTTTCACCTTCCTCGGGCGCGGGAATGACTGAGTCTTTAGTTTCGACGGGATTAAAAATAACATTTATCGTCGATTCTGCTACACCTCG  >  NC_000913/3803221‑3803693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑