Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,781,049	C→T	intergenic (+32/‑166)	lldD → / → trmL	L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,049	0	C	T	94.4%	105.6 / ‑3.9	37	intergenic (+32/‑166)	lldD/trmL	L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (18/16);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACC  >  NC_000913/3780808‑3781289                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTCCTAAATCTGCTAGAAAAAGAGATGAAAGTGGCAATGACGCTGACTGGCGCGAAATCGATTAGCGAAATTTCGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGAATAGGCGTTTGCGCCCCCTCACCCTAAACCTCTCCTT‑C‑Aggg                                                                                                                                                                                                                                             >  1:255056/1‑248 (MQ=255)   gggTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGAGTAGCTAACCTGGTAAAACTGATCGAAAAAGAGGTGAAAGAGGGGATGACACTGAATGGCGGGGAATTGAGCAGGGGAATTACGCCAGACTACG‑TGGGGGAGGGGGTGGGTGAAGAGGTGTCCGCGGCCCAGGGTCCCATGGCGGGAGGGGAGG‑CGGACTGGTCGTTTGCCGCCCCTCACACCAACCCTCTCCTT‑C‑Agggggcgg                                                                                                                                                                                                                                        >  1:267188/1‑246 (MQ=255)        gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGAcc                                                                                                                                                                                                                                   <  1:293088/251‑1 (MQ=255)              tCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCg                                                                                                                                                                                                                              <  1:90219/250‑1 (MQ=255)                    aTGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCtg                                                                                                                                                                                                                        <  1:279801/250‑1 (MQ=255)                                   cGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAct                                                                                                                                                                                                        <  1:84601/251‑1 (MQ=255)                                        cAGGCGGGTGTAGCTAACCTGCTTAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGGGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                                                                                                                    >  2:242355/1‑250 (MQ=255)                                                   aGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                                                                                                        <  1:130487/251‑1 (MQ=255)                                                       aaCCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAct                                                                                                                                                                                      <  1:165884/249‑1 (MQ=255)                                                         ccTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttt                                                                                                                                                                                  >  1:275065/1‑251 (MQ=255)                                                               aaaTCTGATCGACAAAGAGATGAAAGAGGCGATGACGCTGACAGGAGCGCAATCAATAAGCGAAATTACGAAAGA‑TTCAATGGTGCAGGGGCTAGGTAAAGAGATGCCTGAGGCACTGGCTCCCATGGCGCAAGGGAGTG‑CGGCATAGTCGTTTGCGCCCCCTCAACCTAACCCTCTCCTT‑A‑AGGAAGAGGGGACAGTTCGGAGCTGTATGTACTCCCTCACTCTGAAACGACACCGCCATCTTTTTTtct                                                                                                                                                                            >  1:39347/1‑251 (MQ=255)                                                               aaaTCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                                                                            >  1:233596/1‑251 (MQ=255)                                                                aaTCTGAGCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAAGCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCTCCTCACCCTAACCCTCGCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTAGGTACTCCCCCACTCTGAAACGACACCGCCCTCTTTTTTtctc                                                                                                                                                                           <  2:170715/251‑1 (MQ=255)                                                                    tGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTAGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑Tc                                                                                                                                                                      >  2:149537/1‑251 (MQ=255)                                                                        cGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGccc                                                                                                                                                                  <  1:111582/251‑1 (MQ=255)                                                                        cGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGccc                                                                                                                                                                  >  2:285912/1‑251 (MQ=255)                                                                        cGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGccc                                                                                                                                                                  <  1:34896/251‑1 (MQ=255)                                                                         gAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGcccc                                                                                                                                                                 >  1:171899/1‑251 (MQ=255)                                                                               gagaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCggg                                                                                                                                                           <  2:62449/251‑1 (MQ=255)                                                                                    gAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGTGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTCCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGgaga                                                                                                                                                       <  1:133973/250‑1 (MQ=255)                                                                                              aTGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTg                                                                                                                                            >  2:65613/1‑251 (MQ=255)                                                                                                gACGCTGCCTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCtgtatgt                                                                                                                                                                                                                   >  2:301374/1‑179 (MQ=255)                                                                                                gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCtgtatgt                                                                                                                                                                                                                   <  1:301374/179‑1 (MQ=255)                                                                                                   gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGATAGGGCCggg                                                                                                                                               <  2:107468/243‑1 (MQ=255)                                                                                                   gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGATAGGGCCggg                                                                                                                                               >  1:107468/1‑243 (MQ=255)                                                                                                                      aTCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCTGCTCAc                                                                                                                    >  1:262556/1‑251 (MQ=255)                                                                                                                                    gCAAGA‑TACGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGCGGGGACCGTTCGGCGCTGTCTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAACAGGCCGCACTGCTCACCAATTTTCTGCTAt                                                                                                      >  2:106533/1‑251 (MQ=255)                                                                                                                                              gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                                                             >  1:253706/1‑251 (MQ=255)                                                                                                                                                           gggggTAAAGGGTTCCCTGGGGCACGGCCTCCCATGGCAAAAGGGAATG‑CGGCATAGTCTTTTGCGCCCCCCCCCCCTAACCCTCCCCTT‑C‑AGGGGGGGGGGCCCGTCGGGCGCTGTTTGTACTCCCTCACTCTGAAACAACCCCGCACTTTTTTTTTCTCCC‑CCGCCCCCCCGGGGAGAGGCCCGGGGTGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                                                                <  2:233596/249‑1 (MQ=255)                                                                                                                                                             gggaaaaGAGTGGCCGGCGGCACTGGCCCCCGTGG‑GAAAAGGAATGGCGGCAGAGTCGTTGGCCCCCCCCCCCCTTACCCCTC‑CCCTGC‑AGGGAGGGGGGCCCGTCGGGCGGTGTATGTACCCCCTCCCTCGGAAACGACCCCGC‑CTTTTTTTTTCCCCCCTCCCCCCCCCGGGGAGGGGGCGGGGGGGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                                                                              <  2:32083/247‑1 (MQ=255)                                                                                                                                                                             gCGGCACTGGCCCCCAGGGCGAAAGGAAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTC‑CCCT‑CCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACCCCCCCACTCTGAAACGACCCCGCCCTTTTTTTTTCCCCC‑CCCCCCCCCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                                                              <  2:253706/251‑1 (MQ=255)                                                                                                                                                                                          ccATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc                                                   >  2:144089/1‑249 (MQ=255)                                                                                                                                                                                                 gAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc                                          >  2:174196/1‑251 (MQ=255)                                                                                                                                                                                                 aaaaggaagtG‑CGGCATAGT‑GTTTGCCCCCCCTCCCCCCAACCCACCCCGT‑CCGGGGAGGGGGGCCTGTTGGGCGCTGTATGTACCCCCCCGCTCTGAAGCGGCCCCGCCTTCTTTTGTTCCCCC‑TCGCCCCTCCGGGGAGGGGGCGGGGTTGGGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTTCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTGCTTTACGAACCAGAAATTcc                                          <  2:14502/242‑1 (MQ=255)                                                                                                                                                                                                                  gagccgttgccgcCCCCTCCCCGTAACTCCCTCCTC‑C‑AGGGAGAGGGGACCGTTGGGCGCGTTATGGCCCCCCCCACTCGGAAACGACGCGGCCCTTTTTTTTTCCCCG‑TCGCCCCCCGGGGGAGAGGGCGGGGGTGAGGGAAAAAGGCCGCACTGCTCTCCAATTTTCTGCGAGCCTGCCCCCGCACTAAGGGGGCAGGATGCTAGACAGCTTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAc                          <  1:27586/241‑1 (MQ=255)                                                                                                                                                                                                                           gcgcCCCCTCACCCTAACCCTCTCCTT‑C‑AGGGAGGGGGGCCCGTTCGGCGCTGTATGTCCCCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCg                  <  2:75369/250‑1 (MQ=255)                                                                                                                                                                                                                                          aaCCCTCTCCTT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCACTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCAGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATAcc  >  1:35974/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGA‑TTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATG‑CGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCT‑C‑AGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCC‑TCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACC  >  NC_000913/3780808‑3781289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑