Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,713,979	C→T	P110S (CCG→TCG)	yiaC →	peptidyl‑lysine N‑acetyltransferase YiaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,713,979	0	C	T	96.3%	82.6 / ‑3.4	28	P110S (CCG→TCG)	yiaC	peptidyl‑lysine N‑acetyltransferase YiaC
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (12/14);  total (13/15)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

AGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTC  >  NC_000913/3713738‑3714222                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATaa                                                                                                                                                                                                                                            <  2:17170/251‑1 (MQ=255)   cGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAAt                                                                                                                                                                                                                                          <  1:69569/251‑1 (MQ=255)            ggCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCa                                                                                                                                                                                                                                  <  2:255091/250‑1 (MQ=255)               gTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAAGCAATCGGCGATAAATTTTTACCAGGca                                                                                                                                                                                                                              <  1:100649/251‑1 (MQ=255)                           cGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAACCTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTcac                                                                                                                                                                                                                  >  2:151403/1‑251 (MQ=255)                               ggTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTg                                                                                                                                                                                                              >  1:74764/1‑251 (MQ=255)                                             ctCTTACCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATAATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATgg                                                                                                                                                                                                >  2:181697/2‑251 (MQ=255)                                                 tGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATgg                                                                                                                                                                                                <  1:162716/247‑1 (MQ=255)                                                                             gaagaCGGTAAGCTTCTCGGTGTTGTCAGCATTATGGAAGTCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGTCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGGAGCTCCATCCCCACCTGATGCTGGAGGTTTAGCAAAAAAATCAATCTGCGATAAATTTGTACCAGGCACATGGTTATCACTTTGTCGATTCCGCATTGCAGGATGAAACCCAACTACCCACATGGATTAt                                                                                                                                                                <  1:129485/251‑1 (MQ=255)                                                                                gaCGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATg                                                                                                                                                               >  2:238609/1‑249 (MQ=255)                                                                                                                        gATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGAc                                                                                                                     <  1:294709/251‑1 (MQ=255)                                                                                                                                gCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGGGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGAGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTAt                                                                                                             >  2:278340/1‑251 (MQ=255)                                                                                                                                          ttGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGc                                                                                                    <  1:276306/250‑1 (MQ=255)                                                                                                                                                       ggCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGt                                                                                      >  1:306849/1‑251 (MQ=255)                                                                                                                                                         ccGTCAGGCGTGGTATTGTTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGTTGGTGCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTAt                                                                                    <  1:142858/251‑1 (MQ=255)                                                                                                                                                               ggCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCg                                                                              >  2:47068/1‑251 (MQ=255)                                                                                                                                                                     gTATTGGTAAGGCGCTGATGCAGTATGTGCATCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACATGGTTTTCACATTGTCGATTTCGCATGTCAGGATGAGACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGGATTTTTCCAGCCATGCCAGCGCCGTATTACCCTcacaac                                                                        >  1:51377/1‑249 (MQ=255)                                                                                                                                                                          ggTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTc                                                                   <  2:27373/251‑1 (MQ=255)                                                                                                                                                                            tAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTccc                                                                 >  1:54501/1‑251 (MQ=255)                                                                                                                                                                                         cAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTg                                                    <  1:47068/251‑1 (MQ=255)                                                                                                                                                                                           gTATGTGCAGCAGCGCCATCCCCACCTGATGATGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACTGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTggg                                                  >  1:118272/1‑251 (MQ=255)                                                                                                                                                                                               gtgCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGGGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGgaga                                              <  2:121739/251‑1 (MQ=255)                                                                                                                                                                                                    gcagcGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCttt                                         <  1:122270/251‑1 (MQ=255)                                                                                                                                                                                                      agcGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTcc                                                                                                                         <  1:51812/169‑1 (MQ=255)                                                                                                                                                                                                      agcGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTcc                                                                                                                         >  2:51812/1‑169 (MQ=255)                                                                                                                                                                                                                                gTTTATCAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGttttt              >  1:103837/1‑250 (MQ=255)                                                                                                                                                                                                                                      cAAAAAAATCAATCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGCTCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAAt        >  2:75754/1‑250 (MQ=255)                                                                                                                                                                                                                                           aaaTCAAGCGGCGATAAATTTTTAACAGGCACAGGGTTTTCAAATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTGCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTc  <  2:62897/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    AGCGAATTACTGGCGTGACTGCATTCCGCTGGTGCGGGATGCCTATCTTGCCAACGCGCAAAACTGGGTCTGGGAAGAAGACGGTAAGCTTCTCGGTTTTGTCAGCATTATGGAAGGCCGATTTCTGGCAGCGATGTTTGTCGCACCGAAGGCCGTCAGGCGCGGTATTGGTAAGGCGCTGATGCAGTATGTGCAGCAGCGCCATCCCCACCTGATGCTGGAGGTTTATCAAAAAAATCAACCGGCGATAAATTTTTACCAGGCACAGGGTTTTCACATTGTCGATTGCGCATGGCAGGATGAAACCCAACTACCCACATGGATTATGAGCTGGCCGGTGGTTCAAACGCTGTAAGTGTCAGTTCCGGACCGTTGTATTTTTCCAGCCATGCCAGCGCCGTATTACCCGCACAGCCATTCCCCAGCCGCGAGCTGGGGAGATCTTTGGTCAGCACGTTCACTGCGCCGTTTTTACAAATACCGTC  >  NC_000913/3713738‑3714222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑