Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,633,749	C→T	intergenic (‑1159/‑1092)	yhiJ ← / → yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,633,749	0	C	T	100.0%	54.0 / NA	17	intergenic (‑1159/‑1092)	yhiJ/yhiM	DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

AGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTAAAGAAATTTCTCG  >  NC_000913/3633509‑3633989                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 aGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGccc                                                                                                                                                                                                                                        >  1:118350/1‑251 (MQ=255)                         gTACTAAATCTCTAGCCTGTATGATCCCCCAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTCTTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATACAGTAc                                                                                                                                                                                                                >  1:235053/1‑251 (MQ=255)                                      cGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCtt                                                                                                                                                                                                   >  2:230751/1‑251 (MQ=255)                                                                                               cATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATcacaccacac                                                                                                                                          >  1:110148/1‑251 (MQ=255)                                                                                                    tGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTGAATCACACCACACTGtt                                                                                                                                      <  2:204140/250‑1 (MQ=255)                                                                                                     gATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCCATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGttt                                                                                                                                     >  2:253817/1‑250 (MQ=255)                                                                                                              cATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCt                                                                                                                           <  2:226906/251‑1 (MQ=255)                                                                                                               aTTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTa                                                                                                                          <  2:298597/251‑1 (MQ=255)                                                                                                                           gTCTCTCGTCTTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAac                                                                                                               >  1:209410/1‑250 (MQ=255)                                                                                                                           gTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAaca                                                                                                              <  1:7216/251‑1 (MQ=255)                                                                                                                              tctcGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAacac                                                                                                             >  1:258238/1‑249 (MQ=255)                                                                                                                                                                        tttatATGCAGATGAAGCCATCCCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCtt                                                                 >  2:255725/5‑251 (MQ=255)                                                                                                                                                                                              tctTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTc                                           >  1:243647/1‑251 (MQ=255)                                                                                                                                                                                                          aataatTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAACTAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCACCGCCAGTCAGGTTTGAAAtt                               >  2:24099/1‑251 (MQ=255)                                                                                                                                                                                                             aatTCACATAATAAACTCCAACCCTTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTGTGAAATTAcc                            <  1:278733/251‑1 (MQ=255)                                                                                                                                                                                                                  acaTAATGAACTCCAACCCGTTTCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAACCTGTTCCTTAGGAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGGTTGAAATTACCAcgaa                       <  2:250358/251‑4 (MQ=255)                                                                                                                                                                                                                                        tCCCTGATGTCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTAAAGAAATTTCTCg  <  2:287350/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 AGGATATATTTATCACCAAAAATAGTACTAAATCTATCGCCTGTATGATCCCCAAGGAAGATACAAGGGGTTATCGCATCGTCCTTTAAAAAAACATGTTGATGCAACTCATTCAAGATCATGTCTCTCGTATTTAATAAATCGTAGAGCACTTTATTATTTTTATGTTTGTATGCAGATGAAGCCATCTCTTCGTGTTTTAATAATTCACATAATAAACTCCAACCCTTTTCCCTGATGCCTATATGCCCTGAAACGAGCAATGCATAAAGTACAGCGCCTACTCTTCCATCTGTATCGCCAAAATAAGCCGTGTTGGCATTTTGTGGGTTAATCACACCACACTGTTTATAGAAGTCTATTTTTGAAAACACAGATTTAACATAGCTATCAAACTTTTCCTTAGTAAACGTCTCTTTAGAGACATCCCAACGCCAGTCAGGTTTGAAATTACCACTAAGAAGAGTAAAGAAATTTCTCG  >  NC_000913/3633509‑3633989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑