Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,618,836 C→T V83V (GTC→GTT)  nikR → DNA‑binding transcriptional repressor NikR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,618,8360CT97.9% 151.2 / NA 47V83V (GTC→GTT) nikRDNA‑binding transcriptional repressor NikR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/25);  minor base G (0/1);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCT  >  NC_000913/3618589‑3619077
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
tGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCATCATCATCACCACGACCTCTCCGTTGcc                                                                                                                                                                                                                                                >  1:243178/1‑251 (MQ=255)
         tCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCAt                                                                                                                                                                                                                                       >  1:165503/1‑251 (MQ=255)
          cACCATCACGCTTGAGGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCAtg                                                                                                                                                                                                                                      <  2:112166/251‑1 (MQ=255)
              aTCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGc                                                                                                                                                                                                                                   >  1:221217/1‑250 (MQ=255)
              aTCACGCTTGATGACGATTTACCGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGGCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGca                                                                                                                                                                                                                                  <  2:152536/251‑1 (MQ=255)
                cACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGcaca                                                                                                                                                                                                                                <  2:99443/251‑1 (MQ=255)
                cACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGcaca                                                                                                                                                                                                                                <  2:215066/251‑1 (MQ=255)
                cACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGcac                                                                                                                                                                                                                                 >  2:57138/1‑250 (MQ=255)
                   gcgtgatgaCGAGTTAATGGAGACGCTGGACAGACGGAGACAGCGTCGTGGTTATAACAACCGTTCCGAAGCTTTCCGCTACACTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAATCCCGACTTAGCCAGCAGCATTGTCTCCCCCCAGCATCATAACCACGACCTCTCCGTTGCCACGCTGCATGTGCCCATCa                                                                                                                                                                                                                             <  2:85159/248‑1 (MQ=255)
                   gCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATc                                                                                                                                                                                                                              >  2:43106/1‑250 (MQ=255)
                   gCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATc                                                                                                                                                                                                                              >  2:167591/1‑250 (MQ=255)
                    cTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCCAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCCGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCa                                                                                                                                                                                                                             >  2:169888/1‑250 (MQ=255)
                      tgatgaCGATTTACTGGAGCCGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAAcc                                                                                                                                                                                                                          >  1:270624/1‑251 (MQ=255)
                                             tGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCCTGTGCACATCAACCACGACGACTGTCTGGCAATCGcc                                                                                                                                                                                                   >  2:45526/1‑251 (MQ=255)
                                                          ccAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTg                                                                                                                                                                                      <  1:148517/251‑1 (MQ=255)
                                                            aGCGTCGTGGTTCTAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGCGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGAc                                                                                                                                                                                    >  2:293629/1‑251 (MQ=255)
                                                                        ataACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAACCGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCCGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTg                                                                                                                                                                        <  2:223224/251‑1 (MQ=255)
                                                                        ataACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTg                                                                                                                                                                        <  1:167591/251‑1 (MQ=255)
                                                                        ataACAACCGTTCCGAAGCTAGCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGt                                                                                                                                                                         <  2:293254/250‑1 (MQ=255)
                                                                                          cTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGAc                                                                                                                                                      >  1:5501/1‑251 (MQ=255)
                                                                                            aTCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGt                                                                                                                                                    >  1:202678/1‑251 (MQ=255)
                                                                                               cgcgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTAt                                                                                                                                                 <  2:116586/251‑1 (MQ=255)
                                                                                                 cgACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCg                                                                                                                                               <  1:73223/251‑1 (MQ=255)
                                                                                                              aGCGCCCTGCCGCAAGAGGCCACCCAGCAGCACGTCACGCACGGCTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAACCGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCCACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGAGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGcgcgccgc                                                                                                                                  >  2:238380/1‑247 (MQ=255)
                                                                                                                   ccTGGCGCAAGAGGCCACCAAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGc                                                                                                                             <  2:270624/251‑1 (MQ=255)
                                                                                                                      ggCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCAc                                                                                                                           <  1:135718/250‑1 (MQ=255)
                                                                                                                           aaGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGTCCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAAGCGCCTTGTTGAAAGGTGACAGGGGTGACGTGCAGCATTTTGCCGTTGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCAt                                                                                                                     <  1:292493/251‑1 (MQ=255)
                                                                                                                                   aCCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTg                                                                                                             <  1:214178/251‑1 (MQ=255)
                                                                                                                                     ccAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCt                                                                                                           >  2:239974/1‑251 (MQ=255)
                                                                                                                                         cagcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGGCTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCAGTTTGCCGATGACGTTATCGCCCAGCGCGGCGTTCGGCACGGGCATTTGCAGTGCTTGcc                                                                                                       <  2:36472/251‑1 (MQ=255)
                                                                                                                                          agcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGc                                                                                                                 >  2:118808/1‑240 (MQ=255)
                                                                                                                                          agcaCGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGc                                                                                                                 <  1:118808/240‑1 (MQ=255)
                                                                                                                                                 cacgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgaaggaag                                                                                               <  1:184729/251‑1 (MQ=255)
                                                                                                                                                   cgCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGCAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCgacgga                                                                                                 >  2:294169/1‑247 (MQ=255)
                                                                                                                                                       aGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTg                                                                                           >  1:279468/1‑249 (MQ=255)
                                                                                                                                                                      gTCGTATGTGTCTGAACACGAAAAACGCGACTTAGCCAGACGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATGGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGACGGAAGAGTGAGTCTATTATTGGGTcc                                                                          >  2:234738/1‑251 (MQ=255)
                                                                                                                                                                                 aTGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACg                                                               <  2:165503/251‑1 (MQ=255)
                                                                                                                                                                                    aaCACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATc                                                            <  1:9031/251‑1 (MQ=255)
                                                                                                                                                                                     acacGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGGGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATGGAGTCTATTATTGGGTCCTTTGGTGAACGATca                                                           <  1:293629/251‑1 (MQ=255)
                                                                                                                                                                                                gcgACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGcc                                                 >  1:238203/1‑250 (MQ=255)
                                                                                                                                                                                                      tAGCCAGCCGCATTGTATCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCTCGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATGTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCTTTTGGTGAACGATCAACAAAGGGCCACGAGc                                           <  2:231954/250‑1 (MQ=255)
                                                                                                                                                                                                               gCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGAtt                                 <  1:174675/251‑1 (MQ=255)
                                                                                                                                                                                                                 aTTGTCTTCCCCCAGCATCATCACTACGACCTCTCCGTGGCCACGCTGCATGTGCACAGCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTGTGCCGATGACGTTATCGCCCATCGCGGCGTGCGGCACGGTCATTTGCAGTGCTTGTCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGCACGTGCAACAAAGGGCCACTTGCACACCTGATTcc                               <  2:281838/251‑1 (MQ=255)
                                                                                                                                                                                                                                catcatCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTTCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTa                <  1:42303/251‑1 (MQ=255)
                                                                                                                                                                                                                                catcatCACCACGACCTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCt                 >  1:58807/1‑250 (MQ=255)
                                                                                                                                                                                                                                              ccTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTctct  <  2:221217/251‑1 (MQ=255)
                                                                                                                                                                                                                                              ccTCTCCGTTGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTctct  >  1:62923/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 
TGCAACGAGTCACCATCACGCTTGATGACGATTTACTGGAGACGCTGGACAGCCTGAGCCAGCGTCGTGGTTATAACAACCGTTCCGAAGCTATCCGCGACATTCTGCGTAGCGCCCTGGCGCAAGAGGCCACCCAGCAGCACGGCACGCAAGGTTTCGCGGTGCTGTCGTATGTGTATGAACACGAAAAACGCGACTTAGCCAGCCGCATTGTCTCCACCCAGCATCATCACCACGACCTCTCCGTCGCCACGCTGCATGTGCACATCAACCACGACGACTGTCTGGAAATCGCCGTGTTGAAAGGTGACATGGGTGACGTGCAGCATTTTGCCGATGACGTTATCGCCCAGCGCGGCGTGCGGCACGGGCATTTGCAGTGCTTGCCGAAGGAAGATTGAGTCTATTATTGGGTCCTTTGGTGAACGATCAACAAAGGGCCACTAGCACACCTGATTCCTCTCTAAATACCCTATCCGAACCTTCTCT  >  NC_000913/3618589‑3619077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑