Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,590,251	C→T	V221M (GTG→ATG)	ugpA ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,590,251	0	C	T	100.0%	214.6 / NA	65	V221M (GTG→ATG)	ugpA	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/32);  total (33/32)

CAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGT  >  NC_000913/3590004‑3590475                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 cAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTTCACCACCGTCAGCACGATGACGAGGAACATCAACCCCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCGGAACCGGCCCGCCGGACGTTTCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTAc                                                                                                                                                                                                                               <  1:306285/251‑1 (MQ=255)    ccACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACcag                                                                                                                                                                                                                            <  2:36564/251‑1 (MQ=255)      acggacggTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACcagc                                                                                                                                                                                                                           >  1:102446/1‑250 (MQ=255)       cggtcggTGCTCAATCATTGGTAACGCACCTTGCGTTCAACATAGCGGACCTGCGCCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTTAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCATGTTCATTACcagcag                                                                                                                                                                                                                         <  2:268054/247‑1 (MQ=255)       cggacggTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACcagc                                                                                                                                                                                                                           >  1:2953/1‑249 (MQ=255)       cggacggGTCGCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCCCGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACcagcag                                                                                                                                                                                                                         <  2:264136/251‑1 (MQ=255)              ttCTCAATCATTGGTAACGCCCCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGAGCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGGTCATTACCAGCAGgaagaa                                                                                                                                                                                                                   >  2:302456/1‑250 (MQ=255)              ttCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGGGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGgaagaa                                                                                                                                                                                                                   >  2:90549/1‑250 (MQ=255)               tctcAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCCGGAAGAAAc                                                                                                                                                                                                                 >  1:174633/1‑251 (MQ=255)                       aTTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGCTGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGACGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGGGCCGCAGAAGGCACATACCAGGCTCATTACCACCAGGAAGGAACTCACCggg                                                                                                                                                                                                         >  2:93987/1‑251 (MQ=255)                          ggTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCg                                                                                                                                                                                                      <  1:141593/251‑1 (MQ=255)                               cGCACCTTGCTTTCACCATAGCGGAACTGCCCCCCCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAg                                                                                                                                                                                                 <  2:219320/251‑1 (MQ=255)                                gCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGc                                                                                                                                                                                                >  2:79936/1‑251 (MQ=255)                                      tGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCa                                                                                                                                                                                            <  1:235373/249‑1 (MQ=255)                                       gCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcg                                                                                                                                                                                         >  2:120999/1‑251 (MQ=255)                                       gCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcg                                                                                                                                                                                         >  1:275713/1‑251 (MQ=255)                                       gCTTTAAACATAGCGGAACTGCACCACCGTCAGCACGACGACGAGGAACATCAGCACCTCCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCGGGAAGAAACTCACCGGGGCGATAAGCTGCAgcg                                                                                                                                                                                         <  2:202118/251‑1 (MQ=255)                                               cATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTa                                                                                                                                                                                 <  1:7260/251‑1 (MQ=255)                                                             accaccGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                                                                                                                                                                   >  1:153312/1‑251 (MQ=255)                                                                  cGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGcc                                                                                                                                                              >  1:259513/1‑251 (MQ=255)                                                                      aGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGc                                                                                                                                                          <  2:5664/251‑1 (MQ=255)                                                                         aCGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCAcc                                                                                                                                                       >  1:79728/1‑251 (MQ=255)                                                                            aTGACGAGGAACATCAACACAACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTc                                                                                                                                                    >  1:266135/1‑251 (MQ=255)                                                                                cGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATg                                                                                                                                                <  1:162807/251‑1 (MQ=255)                                                                                    gAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTg                                                                                                                                            >  2:238492/1‑251 (MQ=255)                                                                                     aaCATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGc                                                                                                                                           >  1:129571/1‑251 (MQ=255)                                                                                            acacCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCg                                                                                                                                    >  1:186574/1‑251 (MQ=255)                                                                                              cccaccGACTGTGCGGCAGACGAAGCAAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCTGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGAt                                                                                                                                  <  2:304733/250‑1 (MQ=255)                                                                                               ccaccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTc                                                                                                                                                                                                               >  2:63820/1‑173 (MQ=255)                                                                                               ccaccGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTc                                                                                                                                                                                                               <  1:63820/173‑1 (MQ=255)                                                                                                   cGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCaacg                                                                                                                             >  1:277590/1‑251 (MQ=255)                                                                                                    gACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCaacg                                                                                                                             >  2:186048/1‑250 (MQ=255)                                                                                                        gtgCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCaacgaacg                                                                                                                         <  1:302593/250‑1 (MQ=255)                                                                                                              cAGACAAAGCTAGATCCCGGCCGGTAAAAGCTTCGCGGTAGATCATATAAATCTGCGTCGTGGGGGAATGAACCGGCCCGCCGGCCGTTGCGGCGACGATCCCCGGGAAGTGGTCGAAGAAGGCATACACAAGGTTCATTACCAGCAGGAAGAAGCTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGAGGCGAATCGGCCCTGCACCTTCGATGGCTGCGGTTTCGATCACCGAGCGGTGAATg                                                                                                                  <  2:202929/251‑1 (MQ=255)                                                                                                                 aCGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGAt                                                                                                               <  2:115350/251‑1 (MQ=255)                                                                                                                         aGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCCCCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAgcgc                                                                                                       <  1:153083/251‑1 (MQ=255)                                                                                                                           aTCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGACGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCgg                                                                                                     >  2:270951/1‑251 (MQ=255)                                                                                                                              cAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCAt                                                                                                  <  1:270951/251‑1 (MQ=255)                                                                                                                               aGTCCGGTAAAACCTTCGCGGTAGATCTTATAAAGCAGCGTCGTGGTGGCCGGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATa                                                                                                 <  2:223421/251‑1 (MQ=255)                                                                                                                                gTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCAt                                                                                                  >  2:130495/1‑249 (MQ=255)                                                                                                                                       aaaaCCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACa                                                                                         <  1:120999/251‑1 (MQ=255)                                                                                                                                        aaaCCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAAc                                                                                          >  1:188951/1‑249 (MQ=255)                                                                                                                                                 cgGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTa                                                                               <  2:10267/251‑1 (MQ=255)                                                                                                                                                 cgGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGGGTCGAAGAAGGCATACACCAGGGTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGAAGGCTGCGGCTTCGATCAACGCACGGGGAATGGATTGCAGCGCGGCATAGAAGGACAGGAAGTTGt                                                                                >  2:71550/1‑250 (MQ=255)                                                                                                                                                   gaaGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCTGCACGGCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGAGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCGTCGATCAACGCACGGGGACTGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAgg                                                                             >  2:64106/3‑250 (MQ=255)                                                                                                                                                     aGATCTTATAAATCAGCGTCGGGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCCCCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTg                                                                           <  1:128357/251‑1 (MQ=255)                                                                                                                                                        tCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGTCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGAtt                                                                        >  1:28260/1‑251 (MQ=255)                                                                                                                                                         ctgataAATCAACGTCGTGGTGGCCTGAACCGGCCCGCCGGCCGTGGCGGCGTCGATCACCGGTAAGGTGTCGAAGAAGGCATACACCAGGTTCATTAACAGCAGGAAGAAACTCAACGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGACTCGGCCCTGCACCGTCGATGGAGGAGGCCTGGATCAACGAACGGGGAATGGATTGCCGCGCGGCGTAGAAGAACAGGAATTTGTAGCTGCttt                                                                       >  2:71713/4‑251 (MQ=255)                                                                                                                                                          ttATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTg                                                                      >  2:13865/1‑251 (MQ=255)                                                                                                                                                           tataAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGAtt                                                                        <  1:290597/248‑1 (MQ=255)                                                                                                                                                             taAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGAGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCCATGGCTGCGGCCTCGATCAACCACCGGGGAATGGATTGCAGCGCGGCATAGAAGAACCGGAAGTTGTAGCTGATTTGCtt                                                                   >  2:227598/1‑251 (MQ=255)                                                                                                                                                                   aGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATAc                                                             <  1:181240/251‑1 (MQ=255)                                                                                                                                                                    gCGTCGTGGTTGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCTATCACCGGGAAGGTGTCGAAGAAGGCATACTCCAGGGTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGAGGGCTGCGGCTTCGATCAACGAACTGGGAATGTATTGCAGCGCGGCATAGAATAACAGGAAGTTGGAGTGGATTTGCGTCCATACt                                                            <  2:300103/251‑1 (MQ=255)                                                                                                                                                                            gtggCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCt                                                                                                                                                            <  1:102787/147‑1 (MQ=255)                                                                                                                                                                            gtggCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCt                                                                                                                                                            >  2:102787/1‑147 (MQ=255)                                                                                                                                                                                    aaCCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGaa                                                                                                                    >  1:16651/1‑179 (MQ=255)                                                                                                                                                                                    aaCCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGaa                                                                                                                    <  2:16651/179‑1 (MQ=255)                                                                                                                                                                                     aCCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAg                                           <  2:79728/251‑1 (MQ=255)                                                                                                                                                                                                  aCGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTg                               >  2:116946/1‑250 (MQ=255)                                                                                                                                                                                                  aCGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTGa                              <  1:82013/251‑1 (MQ=255)                                                                                                                                                                                                    gTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTGAcc                            <  2:251925/251‑1 (MQ=255)                                                                                                                                                                                                                    ccGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTGACCGCTGTTTTGCGCGTgg            >  1:68803/1‑251 (MQ=255)                                                                                                                                                                                                                        gAAGGTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTGACCGCTGTTTTGCGCGTGGTTct        <  1:1477/251‑2 (MQ=255)                                                                                                                                                                                                                           ggTGTCGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGt     <  1:55728/251‑1 (MQ=255)                                                                                                                                                                                                                                cGAAGAAGGCATACACCAGGTTCATTACCAGCAGGAAGAAACTCACCAGGGCGATAAGCGGCAGCGCAATCTTCAAGAAGCGGCGCATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTTACCGCTGTTTTGCGCGTGGTTCCAGTCGt  >  2:196679/1‑249 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 CAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAAGAACAGGAAGTTGTAGCTGATTTGCTTCCATACTGAGGCAAACACCACCAGAAACATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGT  >  NC_000913/3590004‑3590475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑