Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,589,956	G→A	L24L (CTC→CTT)	ugpE ←	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,589,956	0	G	A	100.0%	194.9 / NA	57	L24L (CTC→CTT)	ugpE	sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/31);  total (26/31)

CGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGT  >  NC_000913/3589711‑3590200                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     cGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGAt                                                                                                                                                                                                                                                 <  2:69364/251‑1 (MQ=255)            aaTGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATcc                                                                                                                                                                                                                                      >  1:219320/1‑251 (MQ=255)               ggCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGa                                                                                                                                                                                                                                   <  1:150422/251‑1 (MQ=255)                 cAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAgg                                                                                                                                                                                                                                 <  1:77992/251‑1 (MQ=255)                            agcagcGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGt                                                                                                                                                                                                                      <  2:234151/251‑1 (MQ=255)                               aTCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGAGTTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCTCGTACAGCGGTAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATg                                                                                                                                                                                                                   <  1:50374/251‑1 (MQ=255)                                       ggTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAat                                                                                                                                                                                                            >  2:285882/1‑250 (MQ=255)                                          aaTTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGACGTGTGCCGGGGATGAGCTTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCg                                                                                                                                                                                                        <  1:296283/251‑1 (MQ=255)                                                gCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGcc                                                                                                                                                                                                  <  2:136746/251‑1 (MQ=255)                                                     gCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCacgga                                                                                                                                                                                             <  1:154280/251‑1 (MQ=255)                                                           tGCTGAACGACATCACACAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAAATGTTGTGGATGTTTTCCAGCAGCTGTGTGCCGGGGATGAGCGACATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTAGCAGCGACAAACGCCACGCACAGCGGGAAAAGGATAACCGCGGTCCCGAGGATCAGCGTGGTATGGACGAATATCGTCAGCAacgggggggt                                                                                                                                                                                        >  2:153083/1‑244 (MQ=255)                                                                   gCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGTTGTTGTGGATGTTTTCCAGCAGATGTGTGCCGTGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATCTCGTCAGCCACGGTCGGTTTtcattcat                                                                                                                                                                               >  1:137131/1‑251 (MQ=255)                                                                     cATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTg                                                                                                                                                                             <  2:254738/251‑1 (MQ=255)                                                                       tCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGt                                                                                                                                                                           >  2:241658/1‑251 (MQ=255)                                                                        cacaAAGCTTTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTTCCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGTATGGGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACATCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGt                                                                                                                                                                           <  2:115814/250‑1 (MQ=255)                                                                            aaGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGc                                                                                                                                                                      >  2:55728/1‑251 (MQ=255)                                                                             aGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGc                                                                                                                                                                      >  2:302593/1‑250 (MQ=255)                                                                              gCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCAc                                                                                                                                                                    <  1:9958/251‑1 (MQ=255)                                                                                    aaGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCt                                                                                                                                                              >  1:115350/1‑251 (MQ=255)                                                                                            accGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCCCCCAGATGTTGTGGATGGTTTCCAGCAGAGGTGTGCCGGGGATGAGCGTCAGCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACAt                                                                                                                                                      <  2:266679/250‑1 (MQ=255)                                                                                             ccGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACAt                                                                                                                                                      >  1:270843/1‑250 (MQ=255)                                                                                               gCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGc                                                                                                                                                   >  2:235373/1‑251 (MQ=255)                                                                                                  aGAACGGCGCGCTATTCGTGCTTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGTTCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTGTCAACATAGCGGa                                                                                                                                                <  2:271753/251‑1 (MQ=255)                                                                                                    aaCGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAAc                                                                                                                                              <  1:202604/251‑1 (MQ=255)                                                                                                      cGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTg                                                                                                                                            <  2:84129/251‑1 (MQ=255)                                                                                                          gagcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGCCGGGTCTCAATCATTGGTAACGCCCCTTTCTTTCAAAATATAGTAACTGcacc                                                                                                                                        >  2:307915/3‑251 (MQ=255)                                                                                                            gcTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGcaccac                                                                                                                                      >  1:241391/1‑251 (MQ=255)                                                                                                             cTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCAGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATCTCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCaccacc                                                                                                                                     >  2:182157/1‑251 (MQ=255)                                                                                                                 tCGTGCCTACCCCGTTCACCCAGAGGTTGGGGATGTTTTCCAGCAGATGTGGGCCGGGGATGGGCGTCATCGGCGCGTCATAGACGGCCTGTTGATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCCCGATCCCGAGGAGCAGCATGGTATGGCTGAATATCGGCAGCCACGGACGGTTCTCACTCATTGGTAACGCTCCTTGCTTTCAACATAGCGGAACTGCACCACCGTc                                                                                                                                  <  2:282233/250‑1 (MQ=255)                                                                                                                  cGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAg                                                                                                                                <  1:226773/251‑1 (MQ=255)                                                                                                                  cGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTGGCTTTCAACATAGCGGAACTGCACCACCGTCAg                                                                                                                                <  1:174357/251‑1 (MQ=255)                                                                                                                   gTGCCTACACCGTTCACACAGATGTTGGGGATGCTTTACAGCAGATGCGCGCCGGGGATGCGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGCAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACCTAGCGGAACTGCACCACCGTCAGc                                                                                                                               >  2:306285/1‑251 (MQ=255)                                                                                                                                  ccccAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAAc                                                                                                                <  1:163658/250‑1 (MQ=255)                                                                                                                                  aCCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAAc                                                                                                                <  1:275387/251‑1 (MQ=255)                                                                                                                                             gtgGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCg                                                                                                                                    <  1:162877/220‑1 (MQ=255)                                                                                                                                             gtgGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCg                                                                                                                                    >  2:162877/1‑220 (MQ=255)                                                                                                                                                gATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTAATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGAACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGACCTGCACCACCGTCAGCACGATGACGACGAACATCAACAACACCg                                                                                                   >  2:24494/1‑250 (MQ=255)                                                                                                                                                    ttttCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACtgt                                                                                              >  2:97490/1‑251 (MQ=255)                                                                                                                                                                 gtgtgCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGaa                                                                                  <  1:211689/250‑1 (MQ=255)                                                                                                                                                                       cGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCATCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTTCTTTCAACATAGCGGAACTGCGCCACCGTCATCACGATGACGAGTAAGATCAACACCACCGAGAGTGCGGCTGACTAAGCCAGAt                                                                           <  2:138721/251‑1 (MQ=255)                                                                                                                                                                        ggggATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGcc                                                                               >  1:100632/1‑246 (MQ=255)                                                                                                                                                                        ggggATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATc                                                                          <  1:26355/251‑1 (MQ=255)                                                                                                                                                                              gAGCGTCGTCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTcc                                                                    <  1:952/251‑1 (MQ=255)                                                                                                                                                                              gAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAACGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTcc                                                                    <  1:241658/251‑1 (MQ=255)                                                                                                                                                                              gAGCGGCATCGGCGTGGCATAGTCGGCCGGTTTATCCAGCGTCGCCGCGACAAACGCCGCGTACAGCGGGAAAAGGATCTCCGCGAGCCCGAGGATCAGCATGGTATGGCTAAATATCGTCAGCCGCGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACGTTGGGGAACTGCACCACCGGCAGTACGGTGGCGAGGAACATCTACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTc                                                                     <  1:33661/250‑1 (MQ=255)                                                                                                                                                                                       cGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAAcc                                                           <  2:133669/251‑1 (MQ=255)                                                                                                                                                                                             ggCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTcgcg                                                     <  1:182157/251‑1 (MQ=255)                                                                                                                                                                                                aTAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGt                                                   >  1:57503/1‑250 (MQ=255)                                                                                                                                                                                                 tAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGt                                                   >  1:125396/1‑249 (MQ=255)                                                                                                                                                                                                               aTCCAGCGTCGCCGCGACAAACGCCACGTATAGCGGGCAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATAGGTAACGAACCTTGCTTTCAACATAGCGGAACTGCCCCACCGTCAGCACGATGACGCGGAACATCACCACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGAAAAACCTTCGCGGTAGATCTTCTAAATCac                                   >  2:128357/1‑250 (MQ=255)                                                                                                                                                                                                                               aCAAACGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTg                    >  1:251925/1‑250 (MQ=255)                                                                                                                                                                                                                                   aCGCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCCCCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCg               <  1:26260/251‑1 (MQ=255)                                                                                                                                                                                                                                     gCCACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGc             >  2:7260/1‑251 (MQ=255)                                                                                                                                                                                                                                      ccACGTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGcc            >  2:297269/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTACAGCGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCccgcc        >  1:5664/1‑251 (MQ=255)                                                                                                                                                                                                                                           gaCAGCGGTAAAAGGATCACCGCGACCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGACCATCAACCCCACCGACTGTGCGGCAGACTAAGCCAGATCCAGTCCGTTAAAACCTTCGTGGTAGATCTTAAAAATCAGCGTCGTGGTGGCCTGAACCGGCccgccg       <  1:127638/250‑1 (MQ=255)                                                                                                                                                                                                                                                cGGGAAAAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGt  >  2:290597/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAAGAGGATCACCGCGATCCCGAGGATCAGCATGGTATGGCTGAATATCGTCAGCCACGGACGGTTCTCAATCATTGGTAACGCACCTTGCTTTCAACATAGCGGAACTGCACCACCGTCAGCACGATGACGAGGAACATCAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGT  >  NC_000913/3589711‑3590200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑