Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,269	C→T	D4N (GAT→AAT)	lipB ←	lipoyl(octanoyl) transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,269	0	C	T	100.0%	66.3 / NA	20	D4N (GAT→AAT)	lipB	lipoyl(octanoyl) transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)

GCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACCGTTGGG  >  NC_000913/662059‑662511                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   gCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAg                                                                                                                                                                                                             <  1:157224/250‑1 (MQ=255)         cgATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGaa                                                                                                                                                                                                     >  1:185569/1‑250 (MQ=255)                   ggATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATca                                                                                                                                                                                           <  2:73764/250‑1 (MQ=255)                                   ccccGGCATTAAAATGTGCGCCGCTTTTCCTGCCTGACCTTGGGTTAATACCGGATAGTGCACGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGGGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGTGGAg                                                                                                                                                                          <  1:238758/250‑1 (MQ=255)                                         cATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTAccc                                                                                                                                                                    >  1:108007/1‑251 (MQ=255)                                                   tgCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGc                                                                                                                                                          >  1:123377/1‑251 (MQ=255)                                                     cTCCGATTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCg                                                                                                                                                        >  2:126516/1‑251 (MQ=255)                                                                   cctgACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGc                                                                                                                                          <  2:185569/251‑1 (MQ=255)                                                                       aCCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGt                                                                                                                                      <  2:123377/251‑1 (MQ=255)                                                                                    aCCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACGAGCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGTAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATg                                                                                                                          <  2:151263/250‑1 (MQ=255)                                                                                                            aTTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGtt                                                                                                 <  1:211888/251‑1 (MQ=255)                                                                                                                          aCTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGtt                                                                                                 <  1:37954/237‑1 (MQ=255)                                                                                                                          aCTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGtt                                                                                                 >  2:37954/1‑237 (MQ=255)                                                                                                                                atcGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAAcc                                                                             >  1:288924/1‑251 (MQ=255)                                                                                                                                  cgcgGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTg                                                                           >  2:226273/1‑251 (MQ=255)                                                                                                                                       gTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGc                                                                                                                                          <  2:13045/183‑1 (MQ=255)                                                                                                                                       gTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGc                                                                                                                                          >  1:13045/1‑183 (MQ=255)                                                                                                                                                                                            gAGCTGGCGGACAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTtgctgatt                 <  1:226273/251‑4 (MQ=255)                                                                                                                                                                                                      aCAAGAATTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGGTACAGAACCATGCGGACAATATCGAGTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCGTTGCTGCTTGGGTTTACCg       <  2:103264/251‑1 (MQ=255)                                                                                                                                                                                                           aaTTTTATTCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACCGTTggg  >  2:138498/1‑251 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   GCCCACCGCGATCGCTCTGGATCACCGGAATATCACCCGGCATTAAAATGTGCTCCGCTTTTCCTGCCTGACCTTGGGTGAATACCGGATAGTGCTCGACCAGCCAGATTTCATCAAGGGTACTATCATCGCGGGTATCGGTGAATTCATGCATAGCCTGGGAGATTGGCTCGTAAGGCTGAAGACCGAGCTGGCGGACAAGAATTTTATCCTGATACAAAACGGCATCTCCGTGGAGAATGAGTAAAAGTGGGGGAAAAGTATATCACAGCGAGGAGAGGGGAGTTACCCGACCAGGAGCCGGGTAACGGAGAAGCGAGTTACAGAACCATGCGGACAATATCGATTTTGCCCAGTTCTTCATACAGTGTTTCAACCTGCTCGATATGAGTGGCGTTGATAGTGATAGATACCGAGTGGTAGTTGCCTTTGCTGCTTGGTTTTACCGTTGGG  >  NC_000913/662059‑662511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑