Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,588,787	G→A	P132S (CCG→TCG)	ugpC ←	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,588,787	0	G	A	100.0%	193.4 / NA	59	P132S (CCG→TCG)	ugpC	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/33);  total (26/33)

TCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAG  >  NC_000913/3588540‑3589024                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              ccGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCg                                                                                                                                                                                                                                            <  1:289216/250‑1 (MQ=255)   ggCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCAAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGAc                                                                                                                                                                                                                                          >  1:110098/1‑251 (MQ=255)     caacaaCGCCGTTCATAACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCCAACAGGAACACCGCCGGATCGCGCACAAGCGCGCGGCCCATCGCCACACGCCGGCGCTGTACGCCGGAAAGCCCGCGCGAGCGACGt                                                                                                                                                                                                                                        >  2:6806/1‑251 (MQ=255)            gccgTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGc                                                                                                                                                                                                                                  >  1:137186/1‑250 (MQ=255)               gTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGAc                                                                                                                                                                                                                              >  2:77866/1‑251 (MQ=255)                    ccaCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGATTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGAGCGCGCACAATCGCGCGGCCCATCGCCACACGCTTGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTcc                                                                                                                                                                                                                         <  1:181654/250‑1 (MQ=255)                       ccATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGc                                                                                                                                                                                                                      <  2:273308/251‑1 (MQ=255)                                 cTGGGCGAGCGTCATCGCTTCACCCTGATCGTGACTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGAGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCGCCAGACTgc                                                                                                                                                                                                            >  2:38816/1‑251 (MQ=255)                                  tGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgcg                                                                                                                                                                                                           >  1:138679/1‑251 (MQ=255)                                       gAGCGTCATCGCTTCAACCTGCTCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGCCGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgccg                                                                                                                                                                                                      >  1:91582/1‑251 (MQ=255)                                              aTCGCTTCACCCTGAGCGTGAGGAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTTCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACCCCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCttt                                                                                                                                                                                               <  2:242382/251‑1 (MQ=255)                                              aTCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCATCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCTCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCttt                                                                                                                                                                                               <  1:265819/251‑1 (MQ=255)                                              aTCGCTTCAACCGGATCGTGAGTAACGTAGAGTGAATTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCttt                                                                                                                                                                                               <  2:69163/251‑1 (MQ=255)                                                        ccTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGCCGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCg                                                                                                                                                                                     >  1:31379/1‑251 (MQ=255)                                                            aTCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCaa                                                                                                                                                                                 <  2:131392/251‑1 (MQ=255)                                                                   gTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTCCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAACGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCCATTtgctg                                                                                                                                                                          >  2:114487/1‑251 (MQ=255)                                                                     aaCGTAGAGTGAAGTCGTTATCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTgctgct                                                                                                                                                                        >  2:173731/1‑251 (MQ=255)                                                                      aCGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCtt                                                                                                                                                                       >  2:56254/1‑251 (MQ=255)                                                                       cGTAGAGTGCAGTCGTTTTCAGGCGCCGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTg                                                                                                                                                                      >  1:105243/1‑251 (MQ=255)                                                                         tAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCTACGTTTGAGCAGACCGTCCAGCTCCAGAATGCTCGCCGCTTCTTTAACGCGCTc                                                                                                                                                                                      <  2:292261/233‑1 (MQ=255)                                                                         tAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTc                                                                                                                                                                                      >  1:292261/1‑233 (MQ=255)                                                                                  gTCGTTTTCAGGCGCCGGGGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCcgcg                                                                                                                                                           <  1:3974/251‑1 (MQ=255)                                                                                          cAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTc                                                                                                                                                    >  1:265523/1‑250 (MQ=255)                                                                                                    tGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGc                                                                                                                                         >  2:231482/1‑251 (MQ=255)                                                                                                     gCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGcc                                                                                                                                        <  1:240695/251‑1 (MQ=255)                                                                                                       aaCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCGGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCAt                                                                                                                                      <  2:154793/251‑1 (MQ=255)                                                                                                       aaCTGTTGCAGTTCAAGAAGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCAt                                                                                                                                      >  1:208129/1‑251 (MQ=255)                                                                                                           gTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGttt                                                                                                                                  <  1:195769/251‑1 (MQ=255)                                                                                                                aGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTGttcttc                                                                                                                             <  1:231482/251‑1 (MQ=255)                                                                                                                    cAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGTTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGac                                                                                                                          >  2:212663/1‑250 (MQ=255)                                                                                                                           cATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCatat                                                                                                                  >  1:79291/1‑251 (MQ=255)                                                                                                                                gcccgcgCAGCTTGGGATCGAGGTTAGAGAGCGTCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCatattcg                                                                                                               <  2:178079/246‑4 (MQ=255)                                                                                                                                gCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGc                                                                                                                <  1:77866/248‑1 (MQ=255)                                                                                                                                  ccgcgCAGCTTGGCAGCGAGGTTAGAGAGCGGCTCAGCAAACAGCAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGGTTGAGCAGACCGTCCAGCTCCAGAGTGCGCGCCGCTTCTTTAACGCGCTCGGCAATGTGCTGCTTGCCCATGCCGCTAATTTTCATCCCCCACGCCGTGTTTTCTGCGACTCTGATATGCGGATa                                                                                                           <  2:29348/250‑1 (MQ=255)                                                                                                                                      gcAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAACCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAgc                                                                                                       <  2:116573/251‑1 (MQ=255)                                                                                                                                      gcAGCTGGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATGTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAgc                                                                                                       <  2:103566/251‑1 (MQ=255)                                                                                                                                            tGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCACTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGt                                                                                                   >  2:223724/1‑249 (MQ=255)                                                                                                                                             ggCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGt                                                                                                <  1:195006/251‑1 (MQ=255)                                                                                                                                              gCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGtt                                                                                               <  1:189708/251‑1 (MQ=255)                                                                                                                                                    aGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCTGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGaa                                                                                         <  2:48726/251‑1 (MQ=255)                                                                                                                                                        tAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACAcc                                                                                     >  1:207023/1‑251 (MQ=255)                                                                                                                                                         agagagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCa                                                                                    <  2:200290/251‑1 (MQ=255)                                                                                                                                                           agagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATc                                                                                  <  1:210168/251‑1 (MQ=255)                                                                                                                                                            gagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCg                                                                                 <  2:110098/251‑1 (MQ=255)                                                                                                                                                               cGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTttc                                                                                                                                >  2:45597/1‑201 (MQ=255)                                                                                                                                                               cGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTttc                                                                                                                                <  1:45597/201‑1 (MQ=255)                                                                                                                                                                      tcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACCCGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAAc                                                                                                                                                                                            >  2:282284/1‑134 (MQ=255)                                                                                                                                                                      tcaAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAAc                                                                                                                                                                                            <  1:282284/134‑1 (MQ=255)                                                                                                                                                                          aCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCt                                                                   <  1:278258/251‑1 (MQ=255)                                                                                                                                                                          aCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCt                                                                   <  1:114065/251‑1 (MQ=255)                                                                                                                                                                           cAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCtt                                                                  <  1:149155/251‑1 (MQ=255)                                                                                                                                                                               gacacCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTgg                                                              >  2:216380/2‑251 (MQ=255)                                                                                                                                                                                 cacCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCt                                                            >  1:84680/1‑251 (MQ=255)                                                                                                                                                                                               gcACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCGCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAAGGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcg                                              <  2:263860/251‑1 (MQ=255)                                                                                                                                                                                                                                   gACCGCCGGAAAGCTCGCGAGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGACCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGACACGCGCAGGTCATTGATCCAGATATCGCCTTCTGTCAcc          >  2:79235/1‑251 (MQ=255)                                                                                                                                                                                                                                      cgccgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTg                                         >  2:79517/1‑217 (MQ=255)                                                                                                                                                                                                                                      cgccgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCGTTTGGCTCCAGTTCAGTCACGCGCTg                                         <  1:79517/217‑1 (MQ=255)                                                                                                                                                                                                                                         cgGAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTcc    <  2:79291/251‑1 (MQ=255)                                                                                                                                                                                                                                           gAAAGCTCGCGCGAGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAg  <  1:160890/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              TCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCCGCTTCTTTAACGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCAG  >  NC_000913/3588540‑3589024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑