Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,587,531	G→A	L195F (CTC→TTC)	ugpQ ←	glycerophosphodiester phosphodiesterase UgpQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,587,531	0	G	A	97.2%	117.4 / NA	36	L195F (CTC→TTC)	ugpQ	glycerophosphodiester phosphodiesterase UgpQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/11);  minor base C (1/0);  total (25/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGATTTCG  >  NC_000913/3587292‑3587775                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     agcaTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGAt                                                                                                                                                                                                                                           >  1:140364/1‑251 (MQ=255)   caTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACCTTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGAGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGACTCACTTATGAttt                                                                                                                                                                                                                                         >  1:71256/1‑250 (MQ=255)   caTATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTGCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTg                                                                                                                                                                                                                                         <  1:43015/251‑1 (MQ=255)                    gagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGCAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGCCGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGc                                                                                                                                                                                                                         >  1:119043/1‑250 (MQ=255)                      gaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACTcagc                                                                                                                                                                                                                      >  2:112593/1‑251 (MQ=255)                          ctgCGACATGCTGAATCAGCCCAGCACGCCGCAGACTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATACACACCCCAACGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACAAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGATATAAATAGAGACGcagccca                                                                                                                                                                                                                   >  1:284423/3‑250 (MQ=255)                          cGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGcagccc                                                                                                                                                                                                                    >  1:284405/1‑249 (MQ=255)                             cGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcg                                                                                                                                                                                                               >  1:292426/1‑251 (MQ=255)                              gACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcgc                                                                                                                                                                                                              >  1:18310/1‑251 (MQ=255)                               aCATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCcgcg                                                                                                                                                                                                             >  2:98241/1‑251 (MQ=255)                                   gCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGt                                                                                                                                                                                                         >  1:177446/1‑251 (MQ=255)                                                aGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGATCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCACCTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGcc                                                                                                                                                                                              >  1:203586/1‑249 (MQ=255)                                                             cTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCAcc                                                                                                                                                                               >  2:2534/1‑250 (MQ=255)                                                                aCATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCAt                                                                                                                                                                            >  1:116792/1‑251 (MQ=255)                                                                              aCTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACcg                                                                                                                                                              >  1:17248/1‑251 (MQ=255)                                                                                       ccGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGt                                                                                                                                                     <  1:21442/251‑1 (MQ=255)                                                                                              gTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGAGGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAATCCGCGCGGCAGTTCCGGTg                                                                                                                                              <  2:175795/251‑1 (MQ=255)                                                                                                 cGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGCGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgcc                                                                                                                                            <  2:166892/250‑1 (MQ=255)                                                                                                 cGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTgcc                                                                                                                                            <  2:166895/250‑1 (MQ=255)                                                                                                                 aTCGCAACGGTACAGATGCCATCCCCACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATACACCAGCATCCGCAGTCCGGCGTCTTTCACCTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCCGTCGTCGCGCCCCTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTTCAGCTTc                                                                                                                           >  2:182882/1‑251 (MQ=255)                                                                                                                        cGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGc                                                                                                                     <  1:96653/250‑1 (MQ=255)                                                                                                                           tACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATc                                                                                                                  >  1:76259/1‑250 (MQ=255)                                                                                                                                     aTCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACg                                                                                                       <  2:236450/251‑1 (MQ=255)                                                                                                                                                            ctgctgCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCa                                                                                  <  1:118071/249‑1 (MQ=255)                                                                                                                                                               ctgcGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCg                                                                             >  1:202674/1‑251 (MQ=255)                                                                                                                                                                              tATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTcgcg                                                               <  1:112028/250‑1 (MQ=255)                                                                                                                                                                              tATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGt                                                              >  1:284195/1‑251 (MQ=255)                                                                                                                                                                                          aaaCCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAgcgc                                                   <  1:256396/250‑1 (MQ=255)                                                                                                                                                                                                    ccGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCCTTTTGc                                        >  2:135314/1‑251 (MQ=255)                                                                                                                                                                                                     cGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGcc                                       <  1:148347/251‑1 (MQ=255)                                                                                                                                                                                                      gCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGccc                                      >  1:286507/1‑251 (MQ=255)                                                                                                                                                                                                                gacTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTTAGATGAATAGAGACGCAGCCCATCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCCGCAACAAACCGCGCGGCAGATCCGGTGCCGCCTGTTGTGCAGCTTCTAACGCATCAATCTCACACGCTGACAGCTGCGGCGGCGTCATACCGGCCCACAGCTCCCGAGCCGCCACCGCCACCCTTTTCCCCGtcaccggcc                            >  2:60744/3‑243 (MQ=255)                                                                                                                                                                                                                  cTTTCAACTGCATCACTCGCGCTTTATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCTCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACCGCTCGCGAGCCGCCAGCGCCCCCCTTTTGCCCGTTAATTGcccc                          >  2:269022/1‑250 (MQ=255)                                                                                                                                                                                                                                     cgcTTTATTGAATAACTTATGCTTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTAAATGGCCCGGTGCCGGTGGCGGGTTTgg       >  2:144892/1‑250 (MQ=255)                                                                                                                                                                                                                                         ttATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGATTTc   >  1:185790/1‑251 (MQ=255)                                                                                                                                                                                                                                          tATTGAATAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGGGGGGTTGATTTCg  >  2:24806/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     AGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTTTATTGAGTAACTTATGATTGAGATGAATAGAGACGCAGCCCAGCCGCGCGGTCAGTTCGCGCCAGTCGTCGCGCCACTCATCCAGCAACAAACCGCGCGGCAGTTCCGGTGCCGCCTGTTGTGCAGCTTCTAAAGCATCAATCTCAAACGATGACAGCAGCGGCGGCGTCATACCGGCCCACAGCTCGCGTGCCGCCAGCGCCACCATTTTGCCCGTTAATGGCCCGGTGCCGGTGGTGGGTTTGATTTCG  >  NC_000913/3587292‑3587775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑