Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,581,316	G→A	G60E (GGA→GAA)	yhhY →	N‑acetyltransferase YhhY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,581,316	0	G	A	100.0%	148.0 / NA	44	G60E (GGA→GAA)	yhhY	N‑acetyltransferase YhhY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

ATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGGAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGA  >  NC_000913/3581074‑3581558                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    aTTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGAcgtcg                                                                                                                                                                                                                                             <  2:170090/251‑1 (MQ=255)       cTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGCGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGcc                                                                                                                                                                                                                                       >  2:132702/1‑251 (MQ=255)              ttGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCa                                                                                                                                                                                                                                 <  1:28689/250‑1 (MQ=255)                gAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCa                                                                                                                                                                                                                              <  1:172658/251‑1 (MQ=255)                          gatgTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCa                                                                                                                                                                                                                   <  2:121346/251‑1 (MQ=255)                                     ggACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCAc                                                                                                                                                                                                        <  2:199414/251‑1 (MQ=255)                                        cATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGCGGCCAGCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGTCTCGCCGATCGTCCCGGCATCAAGCATCTCGTCGCCTTTATTGATGAAGACGTCGTGGGCCATCTCGCCATGGTCG‑TGCAACAGCGCTCAcgcc                                                                                                                                                                                                     <  2:193182/251‑1 (MQ=255)                                                        gACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCg                                                                                                                                                                                      >  1:219119/1‑250 (MQ=255)                                                          cAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGAtt                                                                                                                                                                                   >  1:200764/1‑251 (MQ=255)                                                                aaTGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGTCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTa                                                                                                                                                                             <  2:88453/251‑1 (MQ=255)                                                                        agaTAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTc                                                                                                                                                                     >  1:127043/1‑251 (MQ=255)                                                                        agaTAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTc                                                                                                                                                                     >  2:69956/1‑251 (MQ=255)                                                                           tAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCCGTCATGTTGCCGATTTTGGTATCTGTGTCGAc                                                                                                                                                                  >  2:227595/1‑251 (MQ=255)                                                                            aGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGAct                                                                                                                                                                 >  1:67830/1‑251 (MQ=255)                                                                                           aGAAACACTGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCCGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAAcc                                                                                                                                                  >  1:55505/1‑251 (MQ=255)                                                                                                     gATTACGAGGCCATCAGGCAGATTCACGCACAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTAGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGc                                                                                                                                        >  2:250829/1‑251 (MQ=255)                                                                                                      aTTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGcc                                                                                                                                       <  1:93311/251‑1 (MQ=255)                                                                                                        tACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATCTGTGGCATGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTTTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGcc                                                                                                                                       >  1:82126/1‑249 (MQ=255)                                                                                                                          aTTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgag                                                                                                                       >  2:140436/1‑247 (MQ=255)                                                                                                                          aTTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAgat                                                                                                                   <  2:96897/251‑1 (MQ=255)                                                                                                                            tCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACGCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAAAgatg                                                                                                                  <  2:104041/250‑1 (MQ=255)                                                                                                                              aCGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGCCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGAtt                                                                                                               >  1:165978/1‑251 (MQ=255)                                                                                                                               cGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTg                                                                                                              >  2:109480/1‑251 (MQ=255)                                                                                                                               cGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTg                                                                                                              <  1:235490/251‑1 (MQ=255)                                                                                                                                      ccGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAAtgtg                                                                                                       >  2:113766/1‑251 (MQ=255)                                                                                                                                                aTTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTg                                                                                              <  1:132702/250‑1 (MQ=255)                                                                                                                                                  tGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGCGCGAATGGCCGCTCGTCCCGGCATCAAACAACTCGTAGCCTGTATTGATGAAGACGTCATGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGGATCTGTGTCGCCTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGCGAGATGATTGAAAAGTGCGACAACTGGtt                                                                                           >  1:205715/1‑251 (MQ=255)                                                                                                                                                   gCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGtt                                                                                           <  1:111084/250‑1 (MQ=255)                                                                                                                                                                 gTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCAt                                                                            <  1:246049/251‑1 (MQ=255)                                                                                                                                                                      tCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGACGACGTCGTGGGCCa                                                                                                                                                                                                                                      >  2:244277/1‑93 (MQ=255)                                                                                                                                                                      tCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCAGCAACCAACTCGTCGCCTGTATTGATGAAGACGGCGTGGGCCa                                                                                                                                                                                                                                      <  1:244277/93‑1 (MQ=255)                                                                                                                                                                                          ggCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAAATGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAAc                                                   >  2:218545/1‑251 (MQ=255)                                                                                                                                                                                          ggCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACCCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAAc                                                   >  2:37057/1‑251 (MQ=255)                                                                                                                                                                                             aGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGcc                                                >  2:74249/1‑251 (MQ=255)                                                                                                                                                                                               gAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCTAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGGGTTTGTCGATAACGcccc                                              <  2:264657/251‑1 (MQ=255)                                                                                                                                                                                               gAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGcccc                                              <  1:74249/251‑1 (MQ=255)                                                                                                                                                                                                 gCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCgg                                            <  1:113766/251‑1 (MQ=255)                                                                                                                                                                                                       cGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTTTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAAt                                        <  1:125584/249‑1 (MQ=255)                                                                                                                                                                                                             gcGTCCCGACATCAAGCACCTCGTCGGCTGTATTGATGAAGGCTACGTGTGCCAGCTCACCATTGAGGGTG‑AACAGCGCCCTCGCCGCGGTCATTTTGCCGATTTTTGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGAGGCGAGAGATGAGTCAAAGGTGCGACTACTGGTTACGGGTAGATCGCATTGAACTAACCGGGTTTGCCGATGACGCCCCgtctagtaaagtct                                <  2:284765/250‑14 (MQ=255)                                                                                                                                                                                                                tCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGCCG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATACCGCCCCGGCAATTAAGGTCtat                              >  2:46371/1‑250 (MQ=255)                                                                                                                                                                                                                 cccGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGGAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATaa                            <  2:208320/251‑1 (MQ=255)                                                                                                                                                                                                                       aTCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATa                      <  2:218798/251‑1 (MQ=255)                                                                                                                                                                                                                          aaGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACgg                   >  2:34590/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTTGATGAAGACGTCGTGGGCCATCTAACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGa  <  2:96291/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGTATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGGAGACGTCGTGGGCCATCTCACCATTGACG‑TGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAACTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGA  >  NC_000913/3581074‑3581558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑