Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,576,313	A→G	V250A (GTG→GCG)	gntU ←	low‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,576,313	0	A	G	100.0%	149.0 / NA	43	V250A (GTG→GCG)	gntU	low‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/24);  total (19/24)

CCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCACAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATG  >  NC_000913/3576073‑3576559                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       cccAGAGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGCCAAGCAACCAGAATCGCGGTAACCGGATGCCCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAAcgcgc                                                                                                                                                                                                                                              >  2:245762/1‑251 (MQ=255)                                      gTTTGAACCCGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGgcag                                                                                                                                                                                                          <  1:72668/250‑1 (MQ=255)                                        ttGAACACGCCGCCCGCACCAACCACCAGCAGAATGCTCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGgcagca                                                                                                                                                                                                        <  2:203331/250‑1 (MQ=255)                                         tGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAgg                                                                                                                                                                                                      >  1:48297/1‑251 (MQ=255)                                            acacGCCGCCCGCACCAATCACCAGCAGAATAATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAGTCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATc                                                                                                                                                                                                   <  2:56398/251‑1 (MQ=255)                                               cgccgcCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGc                                                                                                                                                                                                <  1:118640/251‑1 (MQ=255)                                                                                  gccgGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTCACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTc                                                                                                                                                             >  1:171828/1‑251 (MQ=255)                                                                                    cgGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGc                                                                                                                                                           <  2:102624/251‑1 (MQ=255)                                                                                               catGACCGCAAATCTGCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAATCAGAATATCGGTAACCGGATTACCGATAAACTCAAACCATTCGTAAGCAGATGATCCTTCCGGCGCAACCAGCGCGTCATTGGTTTTCAGCCCTCCCATCCCCAACGGCAGCATGCTCAGCAACAAGCTTACGCCGACAGATGGCTTTTTGTCTTCGCCGTGATTCGGt                                                                                                                                                >  2:167967/3‑251 (MQ=255)                                                                                               agTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCgtg                                                                                                                                                >  2:167952/2‑249 (MQ=255)                                                                                                  gACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACGCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTc                                                                                                                                              <  2:44932/250‑1 (MQ=255)                                                                                                            ctcCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATgtcgt                                                                                                                                   >  1:38441/1‑251 (MQ=255)                                                                                                                      ttGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCGCCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATGCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCGTCTCCGAGGTTCGGGTCGCGGAGTTCGTCAGGAata                                                                                                                           <  2:22892/249‑1 (MQ=255)                                                                                                                        ggtttttGGCATGCCCTGGAGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCTCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGAAGCAGGATCAGCGACAGGGTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCa                                                                                                                       <  2:60489/248‑1 (MQ=255)                                                                                                                                    gCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTCCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAAc                                                                                                           >  1:275209/1‑251 (MQ=255)                                                                                                                                     cccTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACg                                                                                                          >  1:288175/1‑251 (MQ=255)                                                                                                                                         gACGCATTGCCAGCCCGTAAATCGCCACCAGACAAGCAACCAGCATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCCGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCCGCTCAACGCACCGGCtg                                                                                                      >  2:54278/1‑251 (MQ=255)                                                                                                                                         gACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCtg                                                                                                      >  2:22982/1‑251 (MQ=255)                                                                                                                                         gACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCtg                                                                                                      <  1:221782/251‑1 (MQ=255)                                                                                                                                           cGCATTGCCAGACAGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATACACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCtggt                                                                                                    >  2:183087/1‑249 (MQ=255)                                                                                                                                                   cAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAATGTAACGGCTGATGAAGTTAc                                                                                            >  1:226409/1‑251 (MQ=255)                                                                                                                                                   cAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTAc                                                                                            <  1:143056/251‑1 (MQ=255)                                                                                                                                                        cGTAAATCGCCACCAGACAAGTAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCcac                                                                                       <  2:235874/251‑1 (MQ=255)                                                                                                                                                            aaTCGCAACCAGACAAGCAACCAGGAACGCGGGAAACGGATGACGGATAAGCTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCTCCGAGATGCGGTGCTCTGATGTCGTCAGGAATATGGAGCTCAACGTAACGGCTGGTGATGATACCCCTCAGCg                                                                                   <  2:135608/251‑1 (MQ=255)                                                                                                                                                               cGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGcc                                                                                >  1:63675/1‑251 (MQ=255)                                                                                                                                                                gCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGCGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGccc                                                                               >  2:236850/1‑251 (MQ=255)                                                                                                                                                                          aaGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATc                                                                     <  2:38441/251‑1 (MQ=255)                                                                                                                                                                           aGCAAACAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGAGCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCATCGACAGGCTTAATCCGAGAGATGTCATTTTGTCGTCGCCGAGATGCGGTGCGCTGATGTGGGGAGGAATATGCTGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCa                                                                    <  1:272742/251‑1 (MQ=255)                                                                                                                                                                                         cgGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCCGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGcc                                                      >  1:30397/1‑251 (MQ=255)                                                                                                                                                                                             aaaCGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGcac                                                  >  2:129111/1‑251 (MQ=255)                                                                                                                                                                                                 ggATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAg                                              <  2:297345/251‑1 (MQ=255)                                                                                                                                                                                                        cGATAAACTCAAACCATTCGTAAGCAGTTGATCCTACCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCGCCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAATGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCATTAATCATTCCCGGAATTGCCGCACACAGGCCTATc                                       <  2:226409/251‑1 (MQ=255)                                                                                                                                                                                                            aaaCTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAgg                                    <  1:6658/250‑1 (MQ=255)                                                                                                                                                                                                                 cAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGatca                                <  1:78518/249‑1 (MQ=255)                                                                                                                                                                                                                 cAAACCATTCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTGCAGCCCGACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTTCCTTCGCCGAGATGCGGTTCGCTGATGTCGGCAGGAATATGCAGCTCAACGTATTGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCGATCAGGatcat                               <  2:153115/250‑1 (MQ=255)                                                                                                                                                                                                                     ccATTCGTAATCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGTCCAATCAGGATCATCCAGc                          <  1:65250/251‑1 (MQ=255)                                                                                                                                                                                                                         tCGTAAGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGGTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACCCAGGCCAATCAGGATCATCCAGCCaa                       >  2:155933/1‑250 (MQ=255)                                                                                                                                                                                                                             aaGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTc                   <  1:113382/250‑1 (MQ=255)                                                                                                                                                                                                                             aaGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCg                  <  2:243050/251‑1 (MQ=255)                                                                                                                                                                                                                              aGCAGTTGATCCTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCgg                 >  1:18438/1‑251 (MQ=255)                                                                                                                                                                                                                                        ccTTCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTg       <  1:109042/251‑1 (MQ=255)                                                                                                                                                                                                                                           tCCGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCg     >  1:285014/1‑250 (MQ=255)                                                                                                                                                                                                                                             cGGCGCAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCGGATGGCGTCAGTAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCTCACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATg  <  1:21055/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       CCCAGTGCCGGACCTACGCCAGAGTCAACCAGCACCTGTTTGAACACGCCGCCCGCACCAATCACCAGCAGAATGATCCCCGCCGGTTGCAGCGCGTGACCGCAAATCTCCATCACTTTGTCTTTTGGCATGCCCTGACGCATTGCCAGACCGTAAATCGCCACCAGACAAGCAACCAGAATCGCGGTAAACGGATGACCGATAAACTCAAACCATTCGTAAGCAGTTGATCCTTCCGGCACAAAACGCGCGGCAATGGTTTTCAGCCCTACCAGCACCAGCGGCAGCAGGATCAGCGACAGGCTGAATCCGAAAGATGGCATTTTGCCTTCGCCGAGATGCGGTTCGCTGATGTCGTCAGGAATATGCAGCTCAACGTAACGGCTGATGAAGTTACCCCACAGCGGCCCGGCAATAATCATTCCCGGAATTGCCGCACACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATG  >  NC_000913/3576073‑3576559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑