Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,119 A→G intergenic (+253/+503) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,1190AG97.5% 130.8 / NA 40intergenic (+253/+503)rtcR/glpGDNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (15/24);  minor base T (0/1);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

GCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCG  >  NC_000913/3559889‑3560364
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
gCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAg                                                                                                                                                                                                                                    <  1:288975/250‑1 (MQ=255)
    cACGCTTCCTGGCGGCGTGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCAGATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAGCCAGCATAACGTGGCTCGATTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACGCTAGCAGCGAGCCGTCGGTATCGATTCCGCTTAACGCCAGAAGGCCGAAAGCAAGGCTGGACTGGGGCATAAAATCgttcgtc                                                                                                                                                                                                                                <  2:177700/250‑7 (MQ=255)
          tACTGGCTGCTGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTTCGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTc                                                                                                                                                                                                                           <  2:229694/249‑1 (MQ=255)
           aCTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGc                                                                                                                                                                                                                         <  2:279956/250‑1 (MQ=255)
                        cGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCa                                                                                                                                                                                                           <  1:93427/251‑1 (MQ=255)
                            ggTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGTAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAAAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATcgc                                                                                                                                                                                                       >  1:257714/1‑251 (MQ=255)
                                        aTCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAAACGAGCGTCGCTTTGCCCATGATCCCGCCATCGCgggcgcggtatt                                                                                                                                                                                           >  2:263989/1‑241 (MQ=255)
                                            aCCATGCTTATGCTGCCCATATTGACCATCTCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCTAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACGGGGAGATAAAATCGAGCGTCGCTGCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGc                                                                                                                                                                                        <  1:31834/250‑1 (MQ=255)
                                             ccATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCa                                                                                                                                                                                      <  1:169361/251‑1 (MQ=255)
                                               atgctgatgctgCCCATATTTACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCAcc                                                                                                                                                                                    <  2:247352/251‑1 (MQ=255)
                                                 gctgatgctgCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGGGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCgg                                                                                                                                                                                  <  2:277739/251‑1 (MQ=255)
                                                            ccATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGTACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAatgatg                                                                                                                                                                       <  1:214995/251‑1 (MQ=255)
                                                                    aCCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGGCGCTTCGCCAATGATCCCGCCCTCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAAtc                                                                                                                                                               >  1:183645/1‑251 (MQ=255)
                                                                         cgcgTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGGGATAAAATCGAGCGTCGCTTCGCCCATGATCCCGCCATCGCGGCTGCGTAATTCGCCCCCGGCGGGAATGAATCGaaaaaagaacg                                                                                                                                                          >  2:63662/1‑244 (MQ=255)
                                                                         cgcgTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAAt                                                                                                                                                                <  1:63662/245‑1 (MQ=255)
                                                                         cgcgTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCCTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGGATGATGCGAAAATcttctt                                                                                                                                                          >  1:19759/1‑251 (MQ=255)
                                                                         cgcgTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAAGGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTAGCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAAtcttct                                                                                                                                                           <  1:306918/250‑1 (MQ=255)
                                                                            gTTACGGCCAAATTTCGAGTTATCGACAACCAGCATAACGGGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGACCTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTa                                                                                                                                                       <  2:186853/251‑1 (MQ=255)
                                                                                     aaaTTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACg                                                                                                                                              <  1:77561/251‑1 (MQ=255)
                                                                                          tCGAGTGATCGACATCCAGCATAACGTGGAGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCCGCGAGCCTTCGCTATCGATGCTGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCCTTTACCGGCAACGTGtt                                                                                                                                          <  2:198707/250‑1 (MQ=255)
                                                                                           cGAGTGATCGACAACCAGCATAACGTGTCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTa                                                                                                                                         <  1:133655/250‑1 (MQ=255)
                                                                                            gAGTGATCGACAACCAGCATAACGGGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGc                                                                                                                                       <  2:123940/251‑1 (MQ=255)
                                                                                                      cAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTgaga                                                                                                                             <  1:124356/251‑1 (MQ=255)
                                                                                                                 aCGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGAc                                                                                                                  <  1:28744/251‑1 (MQ=255)
                                                                                                                        gcgAGGTCTCAATAATGGCGCGTGTGGTGTGACCTTCGTGGTAATCGAACTCCAGCAGCGAGCGGTCGGTGTCGATGCCGCTTATCCCCAGAAGTCCGAAATCAAGGCGGGACTGAGAGATAAAATCGAGCGTCGCTTCGCCTATGATCCCGCCATCTCGGGTTCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGTGATTGTTAGTGACACTgcgc                                                                                                           <  2:168473/251‑1 (MQ=255)
                                                                                                                                           gcgTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCa                                                                                        <  2:226451/251‑1 (MQ=255)
                                                                                                                                                        aCTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTAc                                                                           <  1:71921/251‑1 (MQ=255)
                                                                                                                                                           tCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGGGGTAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCCTCGAGGCTGCGGAATTCGCAACCGGCGAGAAATATTCGAAAATCTTCTTTTACCATTAACTGGTTAGCAACGTTGAGATTATTTGTGGCAATGCGCACATTTCTGTTAtttagtatggcgtgagctacccc                                                                        >  2:296072/1‑230 (MQ=255)
                                                                                                                                                                  aaTCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAAAGATCCCGCCATCGCGGCTGCGTAATTTGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCCTCAACGTGTTAGCAACGTTGAGATTGTTGGGGACAATGCGCAAATTGCTGTGATTGAGCAGGTCGTGCGCTCCCGCCTCCggg                                                                 >  2:137448/1‑250 (MQ=255)
                                                                                                                                                                                    agcagccgcgaTCGATGCAGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGTGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCATCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCTATATCGATa                                               <  2:296197/241‑1 (MQ=255)
                                                                                                                                                                                        gTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGAATGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATACCGCCCTCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGACAATCTTCTTTTACCATCAACGTGTTTGCAACGGTGAGATTGTTGGTGACAATTCGCAAATTGCTGTGATTGAGCCGTGCGTGCGCCACCGCTTCCGGCGTGGTGCCGATTTCGATAAAc                                            >  2:112993/1‑250 (MQ=255)
                                                                                                                                                                                                  aTGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTTGTGAACATGCGCAAATTTCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCCTCGAGcc                                 >  2:75819/1‑251 (MQ=255)
                                                                                                                                                                                                   tGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGAAAAAATTGAGCGTCAGATCGCCAATGATCCCGCCATCCCGGCTGCGTAATTCGCCAACGGGGAGAATGATGAGAAAATCTTTTTTTACCCTCAACGTGTTGGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGATACCGCTTCCGGCGTGGTGCCGCTATGGATAAACAGAGTAGAGcc                                 >  1:181868/1‑250 (MQ=255)
                                                                                                                                                                                                    gCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGCGCGTCGCTTCGCCAATGATCCCGCCATAGCGGCTGCGTCATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCACCGCGTTCGCAACGTTGAGATTGTTGGTGACAATACGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCAAAAAACAGCGGCGAGccct                               >  2:157029/1‑249 (MQ=255)
                                                                                                                                                                                                     ccGCTTATCCCCCGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGGATGATGCGAAAATCTTCTTTTACCCTCAACGGGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTAACGCTTCCGGCGTGGTGCCGATATCGGTAAACAGCGTCGAGCCAtt                              >  2:110680/1‑251 (MQ=255)
                                                                                                                                                                                                       gCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAgtgc                                                                                    <  2:122922/195‑1 (MQ=255)
                                                                                                                                                                                                       gCTTATCCCCAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAgtgc                                                                                    >  1:122922/1‑195 (MQ=255)
                                                                                                                                                                                                               ccAGAATGCCGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGAGTAAATCGCCACCGGCGAGAAAGGAGCGAAAATCTTCTTTTACCCTTCACGGGTTAGCAAAGTTGAGATTGTTGGGGACAATGCGCAAAATGCTGTGATTGAGCAGGGCGGGCGGTACCGCTTCCGGCGGGGTGCCGCTAACGATAAACAGCGTCCGGCCATTGGGGGTTTgt                    >  2:209217/1‑250 (MQ=255)
                                                                                                                                                                                                                       ccGAAATCAAGGCGGGACTGGGAGATAAAATCGAGCGTCGCTTCGCCCATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGcccc            >  1:122258/1‑249 (MQ=255)
                                                                                                                                                                                                                                 ggCGGGACTGTGAGATAAAATCGAGCGTCGCTTCGCTAAGGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAAGGATGCGAAAATCTTCGTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGAAAAGTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGGGGTGCCGATATCGATAAACAGCGTCGAGCCAATGGGGATTTGCTCCTCCACTTTGCGGGCg  <  1:260403/251‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
GCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAATCGAACTCCAGCAGCGAGCCGTCGCTATCGATGCCGCTTATCCCCAGAATGCCGAAATCAAGGCGGAACTGGGAGATAAAATCGAGCGTCGCTTCGCCAATGATCCCGCCATCGCGGCTGCGTAATTCGCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCG  >  NC_000913/3559889‑3560364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑