Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	502,232	G→A	T336I (ACC→ATC)	ybaL ←	putative transporter YbaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	502,232	0	G	A	100.0%	107.6 / NA	33	T336I (ACC→ATC)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/10);  total (23/10)

TCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501995‑502478                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       tCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATTCCGTACg                                                                                                                                                                                                                                           >  2:39101/1‑251 (MQ=255)      tGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTggg                                                                                                                                                                                                                                      >  1:105153/1‑251 (MQ=255)       gggATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTCCGTTggg                                                                                                                                                                                                                                      >  2:104538/1‑250 (MQ=255)          aTCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTg                                                                                                                                                                                                                                  >  1:287937/1‑251 (MQ=255)                           gATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCAcc                                                                                                                                                                                                                  >  1:48333/1‑250 (MQ=255)                             ttGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCCGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAgg                                                                                                                                                                                                               >  2:290159/1‑251 (MQ=255)                                                                    tCGCCAGATTTTTCTCCAGTAGTGCGACCAGTCCCGGTTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGc                                                                                                                                                                         <  1:92690/250‑1 (MQ=255)                                                                    tCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATctcc                                                                                                                                                                        >  2:162632/1‑248 (MQ=255)                                                                          gATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACACTCGCCCCAGGAAAAATGCGACTAACGACTTACCAAACAGAATAATCGCCAGCGTc                                                                                                                                                                  >  2:204036/1‑251 (MQ=255)                                                                                        agtGCAAACATTACCGGGTTGTGCATAATCGCAAGGACCCCCCCTGCCAGTACCAGTTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAAAAGAATAATCGCCAGCGTCGCCAGCACTGCCAg                                                                                                                                                    <  2:286172/251‑1 (MQ=255)                                                                                            caaaCAGTCCCGGGTTGAGCATACTCGACAGGACCCCCCCTGCCAGTACCAGGTTTTGTCCGGCCGGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAAGCTACGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTTCCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGt                                                                                                                                                <  2:210851/249‑1 (MQ=255)                                                                                                           tGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCCAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAtt                                                                                                                                 >  1:71831/1‑251 (MQ=255)                                                                                                            gAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAAtt                                                                                                                                 >  1:225557/1‑250 (MQ=255)                                                                                                                    ccGACAGGACCCCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCa                                                                                                                        <  2:96703/250‑1 (MQ=255)                                                                                                                            atcgccccgcccgcacgaaGGTTTTATCCGGCCTGCGGCCGTAAATTCCATGACATTCCCAATCCCGCCCGGATAAAAGGCAACTAACCAATCCGCGCCAGGCCGGCGCAGCTGACTCACGCAGTACGTTGGGAGTCACCACAAAGCCGCACCAGGAAAAATGCAGCAAACGAATTAACAAACAGAATAATCCCCAGCGTCGCCCGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGCTCAAACAACAt                                                                                                                >  2:20218/19‑251 (MQ=255)                                                                                                                                    tGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACgg                                                                                                        >  1:218040/1‑251 (MQ=255)                                                                                                                                         gTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCTTTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGAc                                                                                                    >  1:33234/1‑250 (MQ=255)                                                                                                                                                   ttGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGc                                                                                          >  2:4030/1‑250 (MQ=255)                                                                                                                                                              gCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCGAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAAcgcg                                                                              >  2:229125/1‑251 (MQ=255)                                                                                                                                                                  cAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACCAAAAACAGAACCGCAAACGCGTcgc                                                                          >  2:165914/1‑251 (MQ=255)                                                                                                                                                                   aGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCGAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTcgcg                                                                         <  1:229125/251‑1 (MQ=255)                                                                                                                                                                          tCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGc                                                                  >  1:128325/1‑251 (MQ=255)                                                                                                                                                                                     cccacccccGCCAGGATAAACGCGAACTCGCCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCTCCAGCACTGCCAGCGGTTTCTGAATCAGAAGTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTg                                                       <  1:39101/245‑1 (MQ=255)                                                                                                                                                                                                   gATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGAc                                         >  1:180589/1‑251 (MQ=255)                                                                                                                                                                                                            gAACTCACCAATCTGCGCCCGGCTGGCGGCGCTGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACAAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGCATCAGAATTAACGGATCAAACAACATCCCGACGGAGACACAAAACAGCACCGCAAACGCGTCGCGCACTGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTc                                  >  2:186955/1‑249 (MQ=255)                                                                                                                                                                                                            gAACTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGAGGGCGGCACGGTGACTCAGTTc                                  >  2:73214/1‑249 (MQ=255)                                                                                                                                                                                                              aCTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGAc                              <  2:229587/251‑1 (MQ=255)                                                                                                                                                                                                               cTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGt                                                                             <  1:98081/203‑1 (MQ=255)                                                                                                                                                                                                               cTCACCAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGt                                                                             >  2:98081/1‑203 (MQ=255)                                                                                                                                                                                                                    cAATCTGCGCCAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTc                        >  2:84237/1‑251 (MQ=255)                                                                                                                                                                                                                              cAGGCTGGCGGCGATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATcc              >  1:33080/1‑251 (MQ=255)                                                                                                                                                                                                                                      cggcgATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAg      <  1:146986/251‑1 (MQ=255)                                                                                                                                                                                                                                         cgATGATTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAAc   <  2:225557/251‑1 (MQ=255)                                                                                                                                                                                                                                          gatgatTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACCACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACg  >  2:261037/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       TCCACTGGGATCTGCTTCTCTTCTTCGATTGCCTCTTCCAGCGTCTGCTCTTCCAGCGTTTCGGTCTTCGCCAGATATTTCTCCAGTAGTGCGAACAGTACCGGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCACTGCCAGCGGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGCAATGGCAGCGTATCGTGGGCGGCACGGTGACTCAGTTCAGACTCGTTCAGTACCATCCCGGCAAAGAACG  >  NC_000913/501995‑502478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑