Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,524,184	C→T	A438A (GCC→GCT)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,524,184	0	C	T	100.0%	182.6 / NA	55	A438A (GCC→GCT)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/31);  total (24/31)

ATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACG  >  NC_000913/3523943‑3524423                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                agccTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTTCTGTTATGgcg                                                                                                                                                                                                                                        <  2:32294/249‑1 (MQ=255)  tCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGgcgc                                                                                                                                                                                                                                       >  1:100374/1‑251 (MQ=255)            gCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTgg                                                                                                                                                                                                                             <  1:204917/251‑1 (MQ=255)                   cGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGGGGCTTTGAt                                                                                                                                                                                                                      >  2:102837/1‑251 (MQ=255)                    gATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGAtt                                                                                                                                                                                                                     <  1:288061/251‑1 (MQ=255)                       gCTGGCGGACGTGTCGACCGTCGCATGGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCGGGGTTCGTCAGGTTGGCGATGCATGTTGGCTGTCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACTGTGCTGTTATGTCGCTGGTCGTTGGCTTTGATTTc                                                                                                                                                                                                                   <  2:278673/250‑1 (MQ=255)                                     cGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGttt                                                                                                                                                                                                    >  2:227015/1‑251 (MQ=255)                                       aCCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTa                                                                                                                                                                                                   >  1:277058/1‑250 (MQ=255)                                          gTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCTGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACcg                                                                                                                                                                                               <  1:238751/251‑1 (MQ=255)                                                ttGAGTATGGAAGGCGTTCACTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCAc                                                                                                                                                                                         >  2:249460/1‑251 (MQ=255)                                                  gAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCAcc                                                                                                                                                                                        <  1:111005/250‑1 (MQ=255)                                                      aTGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCGGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGc                                                                                                                                                                                   <  2:33259/251‑1 (MQ=255)                                                      aTGGAAGGCGTGCGCTGTGCGCGGCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGAGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAAGCCGCAAAACGGTGCTGTTATGGCGCTGTTCGGTGGCTTTGATTGCAATCAGAGCAAGTTTAACCGCGCCCCCCAGGc                                                                                                                                                                                   <  2:216162/251‑1 (MQ=255)                                                           aGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCTCCATCCAGGCACTGc                                                                                                                                                                              <  2:192297/251‑1 (MQ=255)                                                           aGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGGGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGCTTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGc                                                                                                                                                                              >  2:4594/1‑251 (MQ=255)                                                                         cgcgTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTcc                                                                                                                                                                <  1:243815/251‑1 (MQ=255)                                                                          gcgTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCa                                                                                                                                                               <  1:7183/251‑1 (MQ=255)                                                                                           aTACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCctc                                                                                                                                              >  2:92714/1‑251 (MQ=255)                                                                                            tACTCAGCAAGGACCGACGCCGCGGAACGTGACCGCTGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGCGCAAGTTTACCCGCGCCACCCAGGCACTGCGGCAGGTGGGTTCCAAGATCACACCGTTCctct                                                                                                                                             >  2:39636/1‑251 (MQ=255)                                                                                                     aGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCgcgg                                                                                                                                    <  1:83598/251‑1 (MQ=255)                                                                                                      ggACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCgcggc                                                                                                                                   <  1:101313/251‑1 (MQ=255)                                                                                                         ccGACGCCCCTTAAAGTGACCGATGTTCTGCAACCGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCTGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGAt                                                                                                                                <  2:92565/251‑1 (MQ=255)                                                                                                             cgccgcGTAAAGTGCCCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGAt                                                                                                                            >  1:100293/1‑251 (MQ=255)                                                                                                                 gcgTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATaa                                                                                                                          >  1:70308/1‑249 (MQ=255)                                                                                                                    tAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGCTTTCACTCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTACATCATCACACCGTTCCTCTACACCGCGCCGATGGATAAAGGTc                                                                                                                     >  2:82931/1‑251 (MQ=255)                                                                                                                     aaaGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGt                                                                                                                                                   >  2:247753/1‑220 (MQ=255)                                                                                                                     aaaGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGt                                                                                                                                                   <  1:247753/220‑1 (MQ=255)                                                                                                                        gTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGGGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGAc                                                                                                                 <  2:226231/251‑1 (MQ=255)                                                                                                                            ccGATGTTTTGCAAACGGGTCATCAAAGCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCtt                                                                                                             <  2:9992/251‑2 (MQ=255)                                                                                                                              gATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGAGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGc                                                                                                           >  2:83176/1‑251 (MQ=255)                                                                                                                                        aaaCGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTg                                                                                                 >  1:277753/1‑251 (MQ=255)                                                                                                                                              gTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGAt                                                                                           <  2:100374/251‑1 (MQ=255)                                                                                                                                                         tgggtggggggggTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCACTTtc                                                                                >  2:113770/12‑251 (MQ=255)                                                                                                                                                           ggTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTtctc                                                                              >  1:250298/1‑251 (MQ=255)                                                                                                                                                              tCGTCAGGTTGGCGATGAATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGCTAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCt                                                                           >  2:307916/1‑251 (MQ=255)                                                                                                                                                                       tGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCa                                                                   <  1:164420/250‑1 (MQ=255)                                                                                                                                                                          cGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTg                                                               <  1:105648/251‑1 (MQ=255)                                                                                                                                                                          cGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTg                                                               <  1:176551/251‑1 (MQ=255)                                                                                                                                                                                   gtggCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTg                                                      <  1:139095/251‑1 (MQ=255)                                                                                                                                                                                      gctggcACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACt                                                   <  1:7741/251‑1 (MQ=255)                                                                                                                                                                                            acaAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGc                                             >  2:247879/1‑251 (MQ=255)                                                                                                                                                                                                gTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCgaa                                         <  2:256804/251‑1 (MQ=255)                                                                                                                                                                                                   ccGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCAGGTTCTGACTGGCAGCCgaagaa                                      <  1:300471/251‑1 (MQ=255)                                                                                                                                                                                                          tGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTcacc                                >  1:294283/1‑250 (MQ=255)                                                                                                                                                                                                            aaCTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCaccacc                             <  1:84212/251‑1 (MQ=255)                                                                                                                                                                                                            aaCTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCaccacc                             >  2:307830/1‑251 (MQ=255)                                                                                                                                                                                                                cGGCGCTGGGGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGc                           >  2:237348/1‑249 (MQ=255)                                                                                                                                                                                                                    gcTGTTGTGGATCAATCCGCAAAACGGTGCTGTTATGCCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGACCTCCCCACCGCAGTAtt                     <  2:166545/251‑2 (MQ=255)                                                                                                                                                                                                                      tGGTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAACCCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCt                   >  2:95345/1‑251 (MQ=255)                                                                                                                                                                                                                       ggTGTCGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGc                    <  1:21064/249‑1 (MQ=255)                                                                                                                                                                                                                            cGATCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTcc              >  2:281970/1‑250 (MQ=255)                                                                                                                                                                                                                              aTCAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAAt           >  2:217056/1‑251 (MQ=255)                                                                                                                                                                                                                                cAATCCGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGAGGGATAAAGGTCTGACGCTGGCAAGTAGGTTGAACGATGTGCCAATTTCTCGCGGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTc         <  1:189989/251‑1 (MQ=255)                                                                                                                                                                                                                                    ccGCAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCtt     <  2:218597/251‑1 (MQ=255)                                                                                                                                                                                                                                       cAAAACGGTGCTGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACg  <  2:291678/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                ATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGACCGACGCCGCGTAAAGTGACCGATGTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTCGGCGCTGGTGTCGATCAATCCGCAAAACGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTAACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTGACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACG  >  NC_000913/3523943‑3524423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑