Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,270,497	C→T	noncoding (96/377 nt)	rnpB ←	RNase P catalytic RNA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,497	0	C	T	100.0%	60.0 / NA	20	noncoding (96/377 nt)	rnpB	RNase P catalytic RNA component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/9);  total (11/9)

AGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACGC  >  NC_000913/3270266‑3270743                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         aGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCCCTGGTCGTGGG‑ATTTCCCCCCAGGCGGTATCTTG‑CAcccccccccatgt                                                                                                                                                                                                                                      >  1:206219/1‑241 (MQ=255)                    ggCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTCTTGCACTGGTCGTGGGGTTTCCCCCCC‑GGCGGTATCTTG‑CACCCCGCCCCCTGGAGCCCGGCCTTTcctcccc                                                                                                                                                                                                                   >  1:27577/1‑251 (MQ=255)                                          tCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAaga                                                                                                                                                                                              <  1:18173/250‑1 (MQ=255)                                                                               ttGCTCCGGGTGGAGTTAACCGTCCCACGGACTGTTACCAGCCGCGAGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGGCGTGGG‑GTTCCACCCCAGGCGGTATTTGGGCACCC‑CCCCTATGGAGCCCGGCATTTCCTCACCTCCGCCCGGCTTCCCCGCAGAGGGCAACGACGGAGCGGGGGCTGTCTGGGCAgatttgg                                                                                                                                                        >  1:86935/1‑245 (MQ=255)                                                                                                            gACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCtt                                                                                                                          <  1:165510/251‑1 (MQ=255)                                                                                                                  tACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCAc                                                                                                                     <  1:69600/250‑1 (MQ=255)                                                                                                                       gCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGATTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAgcggcg                                                                                                                                                                           <  2:215755/192‑1 (MQ=255)                                                                                                                                   gcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCa                                                                                                   <  2:245027/251‑1 (MQ=255)                                                                                                                                                     cACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCgcggc                                                                                  <  1:243942/250‑1 (MQ=255)                                                                                                                                                       cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCAATTGTCGTGGGGTTTCCCCCCC‑GGCGGTTTCTGG‑CCCCCCGCCCCCTTGAGCCCGGACTTTTCTCCCCCCCCCCCCTCTCCCCCGAAAAGGACGACGACGAAGCGGCGACTGTCTGGGCCGCTTTCGG‑GCGCAGGGTAGAGGGGTTGGGCGCCCTTGGTACCCCCCGGTGCGCATTCCAATctcccggggggcggcggg                                                                               >  1:163562/1‑234 (MQ=255)                                                                                                                                                                         ggcgcGGGCCATCTGCTGTTTGCTCTCTTTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CTCCCTGCCCTATGGAGCCCAGACTTTCCTCCCCTCCGCCCGTCGCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CTGCGTGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTTCGCAGTCCAATCGCCAGTGCCGCGTCGAGATTTCGtcaatctcggta                                                             <  2:42860/250‑12 (MQ=255)                                                                                                                                                                               ggCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATAtt                                                       <  1:119400/251‑1 (MQ=255)                                                                                                                                                                                                     gTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCCCGGAATGCt                                 >  2:229232/1‑251 (MQ=255)                                                                                                                                                                                                         cACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGt                             <  2:269409/251‑1 (MQ=255)                                                                                                                                                                                                         cACTGGCCGTGGG‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCgc                             >  2:202447/1‑250 (MQ=255)                                                                                                                                                                                                                   ggg‑TTTCCCCCCCAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATCAGCCCCGCGGAATGCTTCGTCCAACGTAcc                   >  2:273224/1‑251 (MQ=255)                                                                                                                                                                                                                                cAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCa        >  2:172476/1‑250 (MQ=255)                                                                                                                                                                                                                                cAGGCGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACCTTCCTCCCCTCCGCCCGTCTCCCCCGACGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTAGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCTAACGTACCTATGCTGGTCa        >  1:280694/1‑250 (MQ=255)                                                                                                                                                                                                                                    cGTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACg   >  1:210671/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTTATCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACGc  >  2:11997/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         AGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGG‑TTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTT‑CGGCGCGCAGTATAGAGGGTTTGCGCGCCCTTGTCACCCCGCGTTGCGCATTCCAATCGCCAGTGTCGCGGCGATATTACGTGAAGCACGGCAGATATTGTCATAAGCCCCGCGGAATGCTTCGTCCAACGTACCTATGCTGGTCAATACGC  >  NC_000913/3270266‑3270743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑