Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,219,128	G→A	intergenic (‑52/‑366)	aer ← / → patA	aerotaxis receptor/putrescine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,219,128	0	G	A	100.0%	78.4 / NA	23	intergenic (‑52/‑366)	aer/patA	aerotaxis receptor/putrescine aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/13);  total (10/13)

TGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGTTAACAGATAGCATT  >  NC_000913/3218891‑3219369                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  tGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTg                                                                                                                                                                                                                                       <  2:150803/250‑1 (MQ=255)    cATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGt                                                                                                                                                                                                                                   >  1:121177/1‑251 (MQ=255)                                ggCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGa                                                                                                                                                                                                       <  1:4043/251‑1 (MQ=255)                                                  tGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGAtt                                                                                                                                                                                        <  1:228824/248‑1 (MQ=255)                                                   gCAAGGTATGGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTaa                                                                                                                                                                                     <  2:121177/250‑1 (MQ=255)                                                   gCAAGGTATAGCCGCTCACATGCACAAAAGTGTCATTAGCATGAGTGATATAGCTCTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATAGCAATTTGCGATTTaa                                                                                                                                                                                     >  2:33690/1‑250 (MQ=255)                                                                                          cATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAAGACTTTCACTCTg                                                                                                                                             <  1:54529/251‑1 (MQ=255)                                                                                                tGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACt                                                                                                                                        >  1:84913/1‑250 (MQ=255)                                                                                                    ataGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACg                                                                                                                                   <  2:179483/251‑1 (MQ=255)                                                                                                                  cggaaGTGGACATCAGAGTGGGATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTGCGCGTCCTGTGCAGGGTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTAt                                                                                                                     >  2:5006/5‑251 (MQ=255)                                                                                                                       gTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACGCTGTTACTATACGAAAACGTTAATTATCTTGc                                                                                                                <  1:290933/251‑1 (MQ=255)                                                                                                                           aCATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCaa                                                                                                            <  2:111733/251‑1 (MQ=255)                                                                                                                                 gagTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCa                                                                                                      <  1:3959/251‑1 (MQ=255)                                                                                                                                     ggTATCGTCCGCCAGCGGGGTGTTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCa                                                                                                  <  2:240902/251‑1 (MQ=255)                                                                                                                                                  aGCGGGTTATTTTGCTTGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGa                                                                                     <  1:193724/251‑1 (MQ=255)                                                                                                                                                                              gATGAGAAGACATGTTCGCGTCCTGTGCAGGCAATATGGATGTTCACTTCTTGTAAGAGCTTATATAGGCAACGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGCTCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAAtcatca                                                          >  1:191068/1‑250 (MQ=255)                                                                                                                                                                                             tCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACa                                           <  1:149439/250‑1 (MQ=255)                                                                                                                                                                                              cgcgTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACaa                                          >  1:257684/1‑250 (MQ=255)                                                                                                                                                                                                 gTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAAc                                      <  2:23153/251‑1 (MQ=255)                                                                                                                                                                                                                  tGGTTGTTAACTTCTTGTCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATaacaac                      >  2:957/1‑250 (MQ=255)                                                                                                                                                                                                                   ggTTGTTAACTTCTTGTCAGAGTTTATATCGGCACCGATGCGGGTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAAAACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCTCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGAAATAATCAAATCCATACCCGACAAAAAACGTGCAAAATAacaaca                     >  1:182476/1‑250 (MQ=255)                                                                                                                                                                                                                                   tCAGAGTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGTTAACAGATAGc     >  1:295964/1‑250 (MQ=255)                                                                                                                                                                                                                                     agagTTTATATCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGTTAACAGATAGCAtt  >  1:171923/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  TGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGCTGGGTGACATACGGATGAGAAGACATGTTCGCGTCCTGTGCAGGTTATATGGTTGTTAACTTCTTGTCAGAGTTTATGTCGGCCCCGCTGCGGTTATCTTTAACCGATTAATTTGATTTAGATCGCAATTTGCGATTTAAACACAAATCTAATTCCTTGATTTAAAATACTTTCACTCTGTTACTATACGAAAACGTTAATTATCTTGCCCAAAAATCAGGCAATTATTGCCCTGAAAACGTGCATTTGCGCAGCAATCATCAAATCCATACCCGACAAAAACCGTGCAAAATAACAACAAATGTTAACAGATAGCATT  >  NC_000913/3218891‑3219369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑