Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,263	G→A	A45T (GCC→ACC)	yghT →	putative ATP‑binding protein YghT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,263	0	G	A	100.0%	106.7 / NA	34	A45T (GCC→ACC)	yghT	putative ATP‑binding protein YghT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/11);  total (23/11)

GGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTA  >  NC_000913/3134037‑3134509                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ggTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTg                                                                                                                                                                                                                                 <  2:202612/250‑1 (MQ=255)     aaTTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACaa                                                                                                                                                                                                                             >  1:256599/1‑250 (MQ=255)     aaTTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACaa                                                                                                                                                                                                                             <  2:127311/250‑1 (MQ=255)                      gAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGaa                                                                                                                                                                                                           <  1:212624/251‑1 (MQ=255)                      gAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTGTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGGTCATTTAGGAAAACAGACCGGAAATGGCGGGCGTGCAGTGACAAAATTACCGTTGATggta                                                                                                                                                                                                            <  2:22824/250‑3 (MQ=255)                              cTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATCCATGAAGTCAATAACACCTCAATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGATGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACACAATTACCGTTGATGGGAAAATCCtt                                                                                                                                                                                                    >  2:178592/1‑250 (MQ=255)                                       aaaCATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGAAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAAc                                                                                                                                                                                           >  1:225394/1‑250 (MQ=255)                                            tatTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTAATGAATTCCATAACAGCTCGATTAATTGCCGTTATTGGTAGCGATGGTTAAGGCAAGTCAACGGTGTGTGAACATCTTATTACGGTTGTCGAAAAATATGGTGTTGCTGAACGAGATAATTTAGGCTAACAGACAGGAAAGGGCGGTCGTGCAGTGACAAAATTACCATTGATGGGAAAATGCTGACATAAAACAATTg                                                                                                                                                                                      >  2:112537/1‑250 (MQ=255)                                                             tttttAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAATACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAATTTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGaaa                                                                                                                                                                     >  2:87301/1‑250 (MQ=255)                                                                  aaTGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGc                                                                                                                                                                >  2:219860/1‑250 (MQ=255)                                                                             aaattaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTAGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTTCCGAAAGAGTTCATTTAGGAAAACAGACCGTAAATGTCGGTCGTGCAGTGACAAAATTACCGTGGATGGGAAAATCCTTACATAAGACAATTGAACGAAATCAGGTTAAGACAGCAATAAAATTGcc                                                                                                                                                    <  2:151178/251‑1 (MQ=255)                                                                              aattaaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCt                                                                                                                                                   <  1:262825/251‑1 (MQ=255)                                                                                 taaaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCATgg                                                                                                                                                 >  2:25218/1‑250 (MQ=255)                                                                                   aaGGATATATTCATGCAGTCAATAACACCTCCATTAATTGACGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGAcc                                                                                                                                              >  1:191278/1‑251 (MQ=255)                                                                                            ttCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATCGCCTGGACCAGTTCCGgc                                                                                                                                     >  1:83716/1‑251 (MQ=255)                                                                                               aTGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCt                                                                                                                                  >  1:285900/1‑251 (MQ=255)                                                                                               aTGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCt                                                                                                                                  >  2:208529/1‑251 (MQ=255)                                                                                               aTGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCt                                                                                                                                  >  2:24344/1‑251 (MQ=255)                                                                                                    gTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTa                                                                                                                              >  2:245883/1‑250 (MQ=255)                                                                                                        aTAACACCTCCATTAATTGCAGGTATTGGTAGAGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGGCAAAAGTACCGTGGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGTTGAAAACAGCAAAAAAATGGCCTGGCCCAGTTCCGGCTCTgtttatttc                                                                                                                         <  2:46424/251‑9 (MQ=255)                                                                                                              ccTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGtt                                                                                                                   <  2:134140/251‑1 (MQ=255)                                                                                                                     tAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGTAAAATCCTTACATACAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGcc                                                                                                            >  2:91152/1‑251 (MQ=255)                                                                                                                      aaTTGCCGTTATTGGTAGAGATGGTTCAGGCAAGCCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAACTATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGccc                                                                                                           >  2:288741/1‑251 (MQ=255)                                                                                                                                      aGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGCGTTCATTTAGGAAACCAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATg                                                                                                                                                                                                               >  2:292687/1‑135 (MQ=255)                                                                                                                                      aGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATg                                                                                                                                                                                                               <  1:292687/135‑1 (MQ=255)                                                                                                                                         gATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCg                                                                                        >  2:114129/1‑251 (MQ=255)                                                                                                                                                          aaCGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTgtc                                                                       <  2:295994/251‑1 (MQ=255)                                                                                                                                                                    gAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCgg                                                             >  1:31895/1‑251 (MQ=255)                                                                                                                                                                    gAACATCTTATTACAGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCgg                                                             >  2:31225/1‑251 (MQ=255)                                                                                                                                                                                  cGTTGTCGAAACATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTa                                                >  2:120620/1‑250 (MQ=255)                                                                                                                                                                                  cGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTa                                                >  1:187271/1‑250 (MQ=255)                                                                                                                                                                                               aatGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATcc                                  >  2:12558/2‑251 (MQ=255)                                                                                                                                                                                                            aaaGAGTTCATTTAGGAAAACAGACCGGAAATGTCGGTCGTGCAGTGACAACATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGAGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAAtt                     >  2:262545/1‑251 (MQ=255)                                                                                                                                                                                                                               aCAGACCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTa  <  1:288741/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GGTTAATTGTGACATTGACCTGAATTACGCTTTCATAAAAACATATTAACCAAATAAATATTTTTAATGGATATTTAAATTAAAGGATATATTCATGCAGTCAATAACACCTCCATTAATTGCCGTTATTGGTAGCGATGGTTCAGGCAAGTCAACGGTGTGTGAACATCTTATTACCGTTGTCGAAAAATATGGTGCTGCCGAAAGAGTTCATTTAGGAAAACAGGCCGGAAATGTCGGTCGTGCAGTGACAAAATTACCGTTGATGGGAAAATCCTTACATAAAACAATTGAACGAAATCAGGTGAAAACAGCAAAAAAATTGCCTGGACCAGTTCCGGCGCTGGTAATTACAGCGTTTGTCGCCCGTCGCTTACTGCGCTTTCGTCATATGCTTGCCTGTCGTCGTCGCGGGTTAATTGTTCTAACCGACCGTTATCCTCAGGACCAAATTCCTGGCGCTTACGATGGTA  >  NC_000913/3134037‑3134509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑