Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,121,033	T→C	I83V (ATT→GTT)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,121,033	0	T	C	100.0%	89.9 / NA	27	I83V (ATT→GTT)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

ATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATAT  >  NC_000913/3120795‑3121257                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 aTCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTgg                                                                                                                                                                                                                      >  2:46880/1‑251 (MQ=255)       cacaCCCCCGCCGCGTATAACGGTTTGAAGCCCAGCCCCACCACCAGCTCACCGGTAATCCCCACC‑GGCGCACCAAAGCCACCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGTTGATGGAGATAACCGATCTGCGAATAATAGCAAACTGCCCGCTGGCAACGGTTAATTTATAAATTAACACCGCCGCGACAATAACCCACGCTATTGGCCATaa                                                                                                                                                                                                                <  1:183117/251‑1 (MQ=255)         cacccccGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCCCCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGGAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGACCTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATc                                                                                                                                                                                                              <  2:291072/248‑1 (MQ=255)            ccccGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCat                                                                                                                                                                                                           >  1:207965/1‑251 (MQ=255)                  cgcgTAGAACCGGTTGAAGACCAGGCCCACCCGCAGCGCACCGGTAATCGACACC‑GGCGCACCACAGCCAGCCGCTCCTTCCAGCAACGCACCACAGGCGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGAGATGGAGATAACCGAGATGCGGATAATATCGAACTGCCAGCTGGCAACGGTTAATTTATACAGGACAACCGCAGCGACCATAACCCACGCTATTGGCCATAATCCataaata                                                                                                                                                                                                      >  2:182982/1‑250 (MQ=255)                      aaaaccGGTTTGAAGCCCAGCCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCGGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAAACAGAAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCGGCGGATAATATCGACCTGCCCGCTGGCAAGGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGcc                                                                                                                                                                                                 <  2:272383/246‑1 (MQ=255)                       ataACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAACGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGcc                                                                                                                                                                                                 >  2:259609/1‑250 (MQ=255)                       aaaagggttgaaaGCCCAGCCCCCCCAGCAGCGCACCGGTAATCGCCACC‑GGCCCACAAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCGGCAAACGCTGATCGTCGGTGATGGAGATACCCGAGCTGCGGATAATATCGAACGGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGcc                                                                                                                                                                                                 <  2:135679/239‑1 (MQ=255)                            ggtgtGAAGCCCAGGCCCACCAGCAGCGCACCGGAAATCGCCACC‑GGCGCCCCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCGGATCGTCGGTGATGGAGATAACCGAGCGGCGGATAAAATCGAACGGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGcc                                                                                                                                                                                           <  2:149273/247‑1 (MQ=255)                                tGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCgc                                                                                                                                                                                        >  1:255005/1‑250 (MQ=255)                                tGAAGCCCAGGCCCACCAGCAACGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACAGCCGAGACAATAACCCACGCTATTGGCCATAATCCATAAATAACGCCATAGCCCgc                                                                                                                                                                                        >  2:105500/1‑250 (MQ=255)                                     cccggccccACCACCAGCGCCCCGGTAATC‑CCACCCGGCCCACCAAACCCAGCCGCGCCTTCCAGCACCGAACCAAAGGAGAGACCAACCAGAAACACCTGCAAACGCTGACCGTCGGTTAGGGAGATAACCGAGCTGCGGAAATTATCGACCGGCCCGCTGCCAACGGTTAATTTATACAGCAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCAGAGCCCgcagcagc                                                                                                                                                                                  <  2:299742/245‑1 (MQ=255)                                        aGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCaa                                                                                                                                                                                >  1:22224/1‑250 (MQ=255)                                         ggaccaccaGCAGCGGATCAGTAACCGCCACC‑CCCGCACCAAAGCCAGCCGCCCCTCCCAGCAACGCCCCAAAGGAGAACCCAATCAGTAACACCTCCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGCTAATATCGACCTGCCCGCTGGCAACGGTTAATTTATACCGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATACCCCGCCGCAGCAAAt                                                                                                                                                                              >  2:174973/4‑251 (MQ=255)                                                     cagcacgggaaaTCGCCGCC‑GGAGTACAAAAGCCAGCCTCTCATGCAAGCAACGCACGAAAGAAGAAACAAAAAAGAACCGACTGAAAGCCCTGAGCGTCTGTGATGGAGATACCCGAGCTGCGGAAAATATCGAACTGCCCGTTGGCAAAGGTTAATTAATACAGAAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAAGAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATc                                                                                                                                                                  <  2:288206/241‑1 (MQ=255)                                                        cACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGc                                                                                                                                                               <  1:35984/251‑1 (MQ=255)                                                          ccGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACGGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCAt                                                                                                                                                             <  1:46880/251‑1 (MQ=255)                                                                  cGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAgg                                                                                                                                                     <  1:46356/251‑1 (MQ=255)                                                                           gcccacaaaacccagccGCCCCTTCAAGAAACCCACCAAAGGAAAAACCAATCATGAACCCCTGCAAACGCTGACCGTCGGTGATGGAGATAACCGAGCTGCGAATAATATCGAACGGCCCGCGGGCACCGTTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATAtt                                                                                                                                             <  2:199973/240‑1 (MQ=255)                                                                                                               cAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCATCTGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCa                                                                                                         <  1:215145/251‑1 (MQ=255)                                                                                                                 aaGGAGAAACCAAGCAGTAACACCTGCAAACGCTGATCGTCGGTGAGGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGc                                                                                                       <  1:225086/251‑1 (MQ=255)                                                                                                                                      cccTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTaa                                                                                  <  2:207965/250‑1 (MQ=255)                                                                                                                                                                                cGGATAATATCGAACTGCCCGCTGGCAACGGTTACTTTATACAGGAACAACGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGc                                        >  1:145760/1‑251 (MQ=255)                                                                                                                                                                                  gATAATATCGAACGGCCCGCTGACAGCGGTTAATTTATAAGGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGa                                      <  2:265094/251‑1 (MQ=255)                                                                                                                                                                                    taataTTGAACTGCCTGCCGCCACCGGTTAATTTCAACACAAACACCCCCCCGACATTACCCCACGCCATTGCCCTTAATCCATAAATCAAGCCATAGAACGCACCACCAAATGCCATATCAATCGCCATTTTAACGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGCCGTAATACCGCGAGTGCAACGAAGAAGAATATTCTCGGGATCAGGTCGAcc                                    >  2:28116/1‑251 (MQ=255)                                                                                                                                                                                                               ttAATTTATACAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATc         >  1:96183/1‑251 (MQ=255)                                                                                                                                                                                                                       taCAGGAACACCGCCGCGACAATAACCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCctat  >  2:299578/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                 ATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACC‑GGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTATACAGGAACACCGCCGCGACAATAATCCACGCTATTGGCCATAATCCATAAATAAAGCCATAGCCCGCAGCAGCAAATGCCATATCAATCGGCATTTTAAAGGCGAATATTGCAATCAGGATAGATAATATAAGGGTTATTGCTCCAGCGACATGTCCTTTCAGACGTAATACCGCGAGTGCAACGAAGAAGAATATTATCGGGATCAGGGCGACCAGAGCGGATAGCCCCAGTCCTCCCATCGGCATAT  >  NC_000913/3120795‑3121257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑